Enzymatic degradation of poly-l-lysine-polygalacturonic acid multilayers

Westwood, Marta; Roberts, Dorota; Parker, Roger

doi:10.1016/j.carbpol.2010.12.051

Cited by 17 publications

(19 citation statements)

References 51 publications

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“…In view of the above, the PLL and PGA covered nanocapsuels were selected for further biological studies. It should be mentioned here that those are widely accepted biodegradable polymers [53,54]; It has been well described in the literature, that PLL and PGA are enzymatically digested [55,56]. Since nanoparticles having large surface areas and chemically active surfaces may sometimes interfere with viability assays, producing false assessments of toxicity and making them difficult to analyze [48], we have measured the extent of particle interference in MTT and LDH tests.…”

Section: Cytotoxicity Testsmentioning

confidence: 98%

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Piotrowski

Jantas

Szczepanowicz

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Cytotoxicity Testsmentioning

confidence: 98%

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Piotrowski

Jantas

Szczepanowicz

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…The phenol-sulphuric acid assay, with glucose as a standard, was used to determine the total sugar content, and the modified carbazole and sulphuric acid method, with D-glucuronic acid as the standard, was used to determine the uronic acid content (Masuko et al, 2005;Westwood et al, 2011). The phenol-sulphuric acid assay, with glucose as a standard, was used to determine the total sugar content, and the modified carbazole and sulphuric acid method, with D-glucuronic acid as the standard, was used to determine the uronic acid content (Masuko et al, 2005;Westwood et al, 2011).…”

Section: Molecular Weight Determinationmentioning

confidence: 99%

Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum

Liu

Gong

et al. 2017

Int J of Food Sci Tech

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Summary A crude polysaccharide extract of Dendrobium aphyllum (cDAP, yield 38.15 ± 0.20%) was generated. The D. aphyllum polysaccharide (DAP, Mw 471.586 kDa), purified by DEAE‐Sepharose and Sephadex‐G200 Fast Flow, was composed of mannose (71.3%) and glucose (28.7%), according to GC–MS analysis. Its backbone was composed of β‐d‐mannopyranose and β‐d‐glucopyranose residues, as revealed by infrared spectroscopic analysis. Its glycosidic bond was mainly 1, 4‐linked, and the O‐acetyl groups were mainly linked to mannose residues, according to periodate oxidation and Smith degradation analysis. The DAP units polymerised into a filiform‐shaped spatial pattern, as characterised by atomic force microscopy and scanning electron microscopy. DAP treatment enhanced cytokine secretion (nitric oxide, interleukin‐6 and tumour necrosis factor‐α) and pinocytic and phagocytic capacities of RAW 264.7 mouse macrophages. The complement receptor 3 and mannose receptor were identified to be the receptors of DAP on RAW 264.7 cells, indicating that the Akt/mTOR/MAPK and IKK/nuclear factor‐ĸB pathways could be involved in DAP‐activated immunomodulation.

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“…LbL films have been studied for the development of sensors [4][5][6][7], separation membranes [8], molecular recognition [9,10], and stimuli-sensitive systems [11][12][13][14][15][16][17]. The materials used for the film construction include polysaccharides as well as synthetic polymers [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Use of anionic polysaccharides for the preparation of insulin-containing layer-by-layer films and their pH stability

et al. 2012

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Insulin-containing layer-by-layer (LbL) thin films were prepared by an alternate deposition of insulin and anionic polysaccharides (heparin, j-carrageenan, and fucoidan) through an electrostatic force of attraction between positively charged insulin and anionic polysaccharides at pH 3.0. The loading of insulin in the LbL films increased with the increasing number of layers (or the film thickness), depending on the polysaccharide type. LbL films composed of j-carrageenan contained higher amount of insulin than in heparin-and fucoidan-based films. The LbL films were fairly stable in acidic media, while insulin was released from the films in weakly acidic and neutral solutions as a result of loss of net positive charge in insulin. The released insulin retained its original structure.

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Enzymatic degradation of poly-l-lysine-polygalacturonic acid multilayers

Cited by 17 publications

References 51 publications

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum

Use of anionic polysaccharides for the preparation of insulin-containing layer-by-layer films and their pH stability

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