Enzymatic degradation of lignocellulosic biomass by continuous process using laccase and cellulases with the aid of scaffoldin for ethanol production

Hyeon, Jeong Eun; You, Sung Hyun; Kang, Dae Hee; Ryu, Sun Hwa; Kim, Myungkil; Lee, Sung Suk; Han, Sung Ok

doi:10.1016/j.procbio.2014.05.004

Cited by 25 publications

(7 citation statements)

References 29 publications

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“… BGL1 ( S. fibuligera ); EG ( Clostridium thermocellum ). Barley straw pretreated with laccases complexes 2.3 N.D. [ 153 ] Secretion. BGL1, EG (Truncated, Fibrobacter succinogenes ); CBHII ( Chaetomium thermophilum ).…”

Section: Consolidated Bioprocessingmentioning

confidence: 99%

“…From the works describing conversion of real lignocellulosic biomass, the ones using alkali pretreatments obtained higher ethanol yields, even though there is a lack of a direct comparison study ( Table 1 ). Also, Hyeon and collaborators used an enzymatic pretreatment with laccase complexes to delignify barley straw and produced 2.34 g/L of ethanol with a S. cerevisiae strain secreting endoglucanase and β-glucosidase [ 153 ]. However, from the point of view of an integrated biorefinery, with recovery and utilization of the various fractions of lignocellulosic, these methods are disadvantageous due to the high degradation of hemicellulose and lignin.…”

Section: Consolidated Bioprocessingmentioning

confidence: 99%

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Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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The biorefinery concept, consisting in using renewable biomass with economical and energy goals, appeared in response to the ongoing exhaustion of fossil reserves. Bioethanol is the most prominent biofuel and has been considered one of the top chemicals to be obtained from biomass. Saccharomyces cerevisiae, the preferred microorganism for ethanol production, has been the target of extensive genetic modifications to improve the production of this alcohol from renewable biomasses. Additionally, S. cerevisiae strains from harsh industrial environments have been exploited due to their robust traits and improved fermentative capacity. Nevertheless, there is still not an optimized strain capable of turning second generation bioprocesses economically viable. Considering this, and aiming to facilitate and guide the future development of effective S. cerevisiae strains, this work reviews genetic engineering strategies envisioning improvements in 2 nd generation bioethanol production, with special focus in process-related traits, xylose consumption, and consolidated bioprocessing. Altogether, the genetic toolbox described proves S. cerevisiae to be a key microorganism for the establishment of a bioeconomy, not only for the production of lignocellulosic bioethanol, but also having potential as a cell factory platform for overall valorization of renewable biomasses.

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Section: Consolidated Bioprocessingmentioning

confidence: 99%

Section: Consolidated Bioprocessingmentioning

confidence: 99%

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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“…Also, laccases could possibly address issues regarding phenolic compound inhibition of cellulases. For example, Hyeon et al (2014) achieved 2.6-fold increase in the yield of reduced sugar from pretreated barley straw using cellulase-laccase blends. Moilanen et al (2011) employed blend of commercial cellulases and laccases on pretreated spruce and obtained 12% increase in hydrolysis yield.…”

Section: Biological Additivesmentioning

confidence: 99%

Lignocellulases: a review of emerging and developing enzymes, systems, and practices

Obeng

Adam

Budiman

et al. 2017

Bioresour. Bioprocess.

127

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The highly acclaimed prospect of renewable lignocellulosic biocommodities as obvious replacement of their fossilbased counterparts is burgeoning within the last few years. However, the use of the abundant lignocellulosic biomass provided by nature to produce value-added products, especially bioethanol, still faces significant challenges. One of the crucial challenging factors is in association with the expression levels, stability, and cost-effectiveness of the cellulose-degrading enzymes (cellulases). Interestingly, several recommendable endeavors in the bid to curb these challenges are in pursuance. However, the existing body of literature has not well provided the updated roadmap of the advancement and key players spearheading the current success. Moreover, the description of enzyme systems and emerging paradigms with high prospects, for example, the cell-surface display system has been ill-captured in the literature. This review focuses on the lignocellulosic biocommodity pathway, with emphasis on cellulase and hemicellulase systems. The paradigm shift towards cell-surface display system and its emerging recommendable developments have also been discussed. The attempts in supplementing cellulase with other enzymes, accessory proteins, and chemical additives have also been discussed. Moreover, some of the prominent and influential discoveries in the cellulase fraternity have been discussed.

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“…) . Although many stable cellulases have been identified for cellulose degradation, they are still not considered practical because these biological processes have had slow enzymatic degradation rates . Enzymes are effective and specific biocatalysts that are widely used in industry, but their application in industrial processes often requires desirable functions not found in naturally sourced enzymes .…”

Section: Introductionmentioning

confidence: 99%

“…Enzymes are effective and specific biocatalysts that are widely used in industry, but their application in industrial processes often requires desirable functions not found in naturally sourced enzymes . However, the hydrolysis process using cellulolytic enzymes by conventional expression from cellulolytic microbes is regarded as not cost effective . To obtain the desired enzymes, scientists have developed both rational and non‐rational design methods to enhance enzyme properties .…”

Section: Introductionmentioning

confidence: 99%

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

Hyeon

Shin

Han

2016

Biotechnology Journal

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The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology applications in biorefinery fields, which can be achieved by optimizing the function of various enzymes. This review presents various current scaffolding techniques based on proteins, microbes and nanomaterials for enzyme immobilization, as well as the impact of these techniques on the biorefinery of lignocellulosic materials. Among them, architectural scaffolds have applied to useful strategies for protein engineering to improve the performance of immobilized enzymes in several industrial and research fields. In complexed enzyme systems that have critical roles in carbon metabolism, scaffolding proteins assemble different proteins in relatively durable configurations and facilitate collaborative protein interactions and functions. Additionally, a microbial strain, combined with designer enzyme complexes, can be applied to the immobilizing scaffold because the in vivo immobilizing technique has several benefits in enzymatic reaction systems related to both synthetic biology and metabolic engineering. Furthermore, with the advent of nanotechnology, nanomaterials possessing ideal physicochemical characteristics, such as mass transfer resistance, specific surface area and efficient enzyme loading, can be applied as novel and interesting scaffolds for enzyme immobilization. Intelligent application of various scaffolds to couple with nanoscale engineering tools and metabolic engineering technology may offer particular benefits in research.

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Enzymatic degradation of lignocellulosic biomass by continuous process using laccase and cellulases with the aid of scaffoldin for ethanol production

Cited by 25 publications

References 29 publications

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

Lignocellulases: a review of emerging and developing enzymes, systems, and practices

Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization

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