“…Analysis of mono-and oligosaccharides was performed by using HPAEC-PAD, using CarboPac PA20 (060142, Dionex CarboPac PA20 BioLC, 3 × 150 mm, ThermoFisher Scientific, Sunnyvale, CA, USA) and PA200 (062896, Dionex CarboPac Pa200 BioLC, 3 × 250 mm, ThermoFisher Scientific, Sunnyvale, CA, USA) analytical columns coupled to guard columns of the same material on a Dionex ICS-5000 system (ThermoFisher Scientific, Sunnyvale, CA, USA) according to previously described methods [16,40]. Standards of appropriate monosaccharides, including mannose (M1) or galactose (G1) (Sigma Aldrich, St Louis, MO, USA); linear mannan-oligosaccharides (MOS), including mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5); and galactosylated mannan-oligosaccharides (GMOS), including galactosyl mannotriose (GM3) and di-galactosyl mannopentaose (G2M5), were used (Megazyme, Bray, Ireland) (2.5-50 µM mannose (M1) or galactose (G1) for PA20, 2.5-30 µM M1-M5 and GMOS for PA200).…”