Enzymatic Clipping of DNA Wires Coated with Magnetic Nanoparticles

Kinsella, Joseph M.; Ivanisevic, Albena

doi:10.1021/ja043865b

Cited by 73 publications

(55 citation statements)

References 13 publications

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“…Participation of DNA base groups in binding to nanoparticles would lead to denaturation of DNA double strand. However, free reaction of ethidium bromide (intercalation dye) with CoFe 2 O 4 -DNA can be regarded as an evidence of retention of the double strand structure of the polynucleotide component of the composite with nanoparticles [6]. Hence, it is more likely that mainly PO 2 groups of the DNA sugar-phosphate backbone are involved in the formation of the bond.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

2010

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DNA was immobilized on ferrimagnetic particles of cobalt ferrite nanopowder (CoFe(2)O(4)) and its resistance to endonuclease (DNase I) hydrolysis was studied. Immobilization on cobalt ferrite nanoparticles prevented enzymatic cleavage of DNA. This process was not associated with enzyme inactivation under the effect of nanosize cobalt ferrite and was presumably determined by lesser availability of the DNA molecule as a result of its interaction with nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

2010

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“…EcoRI cuts NP-coated lambda phage DNA into five fragments (3530, 4878, 5804, 7421, and . [50] It is speculated that each fragment of NP chain in solution induces its own local magnetic field. Therefore, the increased number of inhomogeneous local magnetic fields in the solution induced protons to relax faster than in the magnetic fields produced by uncut NP chains.…”

Section: Relaxation Times After Enzyme Manipulation Of Multicomponentmentioning

confidence: 99%

Mechanism of Proton Relaxation for Enzyme‐Manipulated, Multicomponent Gold–Magnetic Nanoparticle Chains

et al. 2010

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Longitudinal and transverse relaxation times of multicomponent nanoparticle (NP) chains are investigated for their potential use as multifunctional imaging agents in magnetic resonance imaging (MRI). Gold NPs (ca. 5 nm) are arranged linearly along double-stranded DNA, creating gold NP chains. After cutting gold NP chains with restriction enzymes (EcoRI or BamHI), multicomponent NP chains are formed through a ligation reaction with enzyme-cut, superparamagnetic NP chains. We evaluate the changes in relaxation times for different constructs of gold-iron oxide NP chains and gold-cobalt iron oxide NP chains using 300 MHz (1)H NMR. In addition, the mechanism of proton relaxation for multicomponent NP chains is examined. The results indicate that relaxation times are dependent on the one-dimensional structure and the amount of superparamagnetic NP chains present in the multicomponent constructs. Multicomponent NP chains arranged on double-stranded DNA provide a feasible method for fabrication of multifunctional imaging agents that improve relaxation times effectively for MRI applications.

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“…Magnetic nanoparticles have been widely used in such fields as catalysis, magnetic resonance image, and controlled drug release because of their special physical property [1][2][3][4]. Magnetic nanoparticles have been synthesized with a number of different compositions, such as Fe 3 O 4 , spinel-type ferromagnets, and alloys.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Fe3O4@SiO2@polymer nanoparticles for controlled drug release

Gao

et al. 2010

Sci. China Chem.

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Novel multifunctional nanoparticles containing a magnetic Fe 3 O 4 @SiO 2 sphere and a biocompatible block copolymer poly(ethylene glycol)-b-poly(aspartate) (PEG-b-PAsp) were prepared. The silica coated on the superparamagnetic core was able to achieve a magnetic dispersivity, as well as to protect Fe 3 O 4 against oxidation and acid corrosion. The PAsp block was grafted to the surface of Fe 3 O 4 @SiO 2 nanoparticles by amido bonds, and the PEG block formed the outermost shell. The anticancer agent doxorubicin (DOX) was loaded into the hybrid nanoparticles via an electrostatic interaction between DOX and PAsp. The release rate of DOX could be adjusted by the pH value. magnetic nanoparticle, Fe 3 O 4 @SiO 2 , multifunctional, controlled drug release

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Enzymatic Clipping of DNA Wires Coated with Magnetic Nanoparticles

Cited by 73 publications

References 13 publications

Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

Mechanism of Proton Relaxation for Enzyme‐Manipulated, Multicomponent Gold–Magnetic Nanoparticle Chains

Synthesis of Fe3O4@SiO2@polymer nanoparticles for controlled drug release

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