Enzymatic catalysis in organic solvents: Polyethylene glycol modified hydrogenase retains sulfhydrogenase activity in toluene

Woodward, Charlene A.; Kaufman, Eric N.

doi:10.1002/(sici)1097-0290(19961105)52:3<423::aid-bit8>3.0.co;2-m

Cited by 21 publications

(9 citation statements)

References 24 publications

(17 reference statements)

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“…Solid supports show that the porous characteristics emerge as optional platforms for enzyme immobilization. These materials include, but are not restricted to, bulk materials (Sheldon, 2007), particles (Ansari and Husain, 2012), hydrogels (Lee et al, 2007), graphene oxide (Pavlidis et al, 2014), carbon nanotubes (Feng and Ji, 2011), mesoporous silica (Hudson et al, 2008;Hartmann and Jung, 2010;Magner, 2013), and polymers (Woodward and Kaufman, 1996;Veronese, 2001). The immobilization of enzymes on these materials may inevitably lead to a low enzyme loading, enzyme denaturation, or restricted mass transfer.…”

Section: Introductionmentioning

confidence: 99%

Metal-Organic Frameworks: A Potential Platform for Enzyme Immobilization and Related Applications

Xia

Zhong

et al. 2020

Front. Bioeng. Biotechnol.

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Enzymes, as natural catalysts with remarkable catalytic activity and high region-selectivities, hold great promise in industrial catalysis. However, applications of enzymatic transformation are hampered by the fragility of enzymes in harsh conditions. Recently, metal–organic frameworks (MOFs), due to their high stability and available structural properties, have emerged as a promising platform for enzyme immobilization. Synthetic strategies of enzyme-MOF composites mainly including surface immobilization, covalent linkage, pore entrapment and in situ synthesis. Compared with free enzymes, most immobilized enzymes exhibit enhanced resistance against solvents and high temperatures. Besides, MOFs serving as matrixes for enzyme immobilization show extraordinary superiority in many aspects compared with other supporting materials. The advantages of using MOFs to support enzymes are discussed. To obtain a high enzyme loading capacity and to reduce the diffusion resistance of reactants and products during the reaction, the mesoporous MOFs have been designed and constructed. This review also covers the applications of enzyme-MOF composites in bio-sensing and detection, bio-catalysis, and cancer therapy, which is concerned with interdisciplinary nano-chemistry, material science and medical chemistry. Finally, some perspectives on reservation or enhancement of bio-catalytic activity of enzyme-MOF composites and the future of enzyme immobilization strategies are discussed.

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Section: Introductionmentioning

confidence: 99%

Metal-Organic Frameworks: A Potential Platform for Enzyme Immobilization and Related Applications

Xia

Zhong

et al. 2020

Front. Bioeng. Biotechnol.

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“…The PEG-LiP was partially soluble in pure MeCN (as indicated by the absorbance peak at 409 nm in MeCN contacted with lyophilized PEG-LiP powder), while no native LiP was found dissolved in MeCN. In an earlier study conducted in this lab, it was also observed that PEGmodified hydrognenase was partially soluble in pure toluene (Woodward and Kaufman, 1996). These observations indicate that the attachment of PEG can effectively increase the compatibility between the enzyme and these organic solvents.…”

Section: Modification Of Ligninasementioning

confidence: 63%

“…Over the past decade, various methods have been investigated to achieve enhanced enzyme activity and stability in such unnatural environments. In particular, grafting amphiphilic macromolecules such as poly(ethylene glycol) (PEG, also referred to as poly(ethylene oxide), PEO) appeared to be a feasible technology for the solubilization and activation of enzymes in organic solvents (Pina et al, 1989;Takahashi et al, 1985;Vazquez-Duhalt et al, 1992;Woodward and Kaufman, 1996). Previous studies on nonaqueous enzymatic catalysis have been primarily focused on biotransformation reactions for chemical production or purification, while applications for remediation of environmental pollutants have been largely ignored.…”

Section: Introductionmentioning

confidence: 99%

Poly(ethylene glycol)-modified ligninase enhances pentachlorophenol biodegradation in water-solvent mixtures

Wang

Woodward

Kaufman

1999

Biotechnol. Bioeng.

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Polychlorinated hydrocarbons are prevalent environmental contaminants whose rates of biodegradation are limited by their minimal solubilities in aqueous solutions where the biological reactions take place. In this study, ligninase (LiP) from Phanerochaete chrysosporium was modified by poly(ethylene glycol) to enhance its activity and stability for the biodegradation of pentachlorophenol (PCP) in the presence of acetonitrile (MeCN), a water-miscible solvent. The modified enzyme retained 100% of its activity in aqueous solutions and showed enhanced tolerance against the organic solvent. The activity of the modified enzyme was found to be over twice that of the native enzyme in the presence of 10% (v/v) MeCN. The solubility of PCP was enhanced significantly by the addition of MeCN to aqueous solutions, such that it was over 10-fold more soluble in the presence of 15% (v/v) MeCN than in pure aqueous buffer solution (from 0.06 to 0.65 mM). Capitalizing on the enhanced substrate solubility and the increased activity of the modified enzyme, the catalytic efficiency of the modified LiP in solutions containing 15% MeCN was over 11fold higher than that of the native enzyme in buffer solutions (pH 4.2) in unoptimized reactor systems (from 44 to 480 mol PCP/mol LiPиh). Continued research both in the use of organic solvents to increase the availability of recalcitrant contaminants and in the modification of enzymes to enhance their activity and stability in such solvents promises to dramatically affect our ability to remediate contaminated sites. Published by John Wiley & Sons

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“…2.4 Determinations of biomass and hydrophobicity of P. aeruginosa 2.4.1 Biomass of P. aeruginosa The biomass of P. aeruginosa was determined by the absorbance of its cell culture medium at the wavelength of 600 nm by Beijing Puxi TU-1950 ultraviolet-visible spectrophotometer (UV-Vis) [27].…”

Section: Culture Conditionsmentioning

confidence: 99%

Action modes of surfactants on biodegradation of Wudong low-rank coal by Pseudomonas aeruginosa

Liu

Shi

et al. 2022

Preprint

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Adding surfactants is an effective method to improve the biodegradation rate of coal. In this study, the action modes of nonionic Triton X-100, anionic surfactant SDS and cationic surfactant DTAB on the biodegradation of Xinjiang Wudong low-rank coal by Pseudomonas aeruginosa were investigated. By measuring cell membrane permeability and cell surface hydrophobicity of Pseudomonas aeruginosa, it was found that the permeability of cell membrane was enhanced under the action of Triton X-100, and reached the highest at 4 CMC, while SDS and DTAB inhibited the permeability of cell membrane. Cell surface hydrophobicity increased under the influence of Triton X-100, SDS and DTAB and reached a maximum at CMC. According to the effects of surfactants on the degradation rates of coal, it was shown that the improvement of cell membrane permeability could promote the degradation of coal, but the increase of cell surface hydrophobicity could inhibit the degradation of coal. When Triton X-100, SDS and DTAB were all at their optimal concentrations for the growths of Pseudomonas aeruginosa, the coal biodegradation rates reached 78.63 %, 55.93 % and 20.93 %, respectively. The adsorption experiment of bacteria on the coal surface and the low-temperature nitrogen adsorption experiment of coal presented that the improvement of permeability of cell membrane was the main factor for Triton X-100 to improve the coal degradation rate, even if it can slightly increase cell surface hydrophobicity. For SDS and DTAB, the decrease of cell membrane permeability and the increase of cell surface hydrophobicity are all not conducive to the biodegradation of coal.

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Enzymatic catalysis in organic solvents: Polyethylene glycol modified hydrogenase retains sulfhydrogenase activity in toluene

Cited by 21 publications

References 24 publications

Metal-Organic Frameworks: A Potential Platform for Enzyme Immobilization and Related Applications

Metal-Organic Frameworks: A Potential Platform for Enzyme Immobilization and Related Applications

Poly(ethylene glycol)-modified ligninase enhances pentachlorophenol biodegradation in water-solvent mixtures

Action modes of surfactants on biodegradation of Wudong low-rank coal by Pseudomonas aeruginosa

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