Enzymatic Biosynthesis of Oligosaccharides and Glycoconjugates

Abstract

Natural carbohydrates have complex structures including many kinds of monosaccharide units, which are very difficult and costly to generate by chemical synthetic approaches. Over the years, enzymatic approaches have been gaining popularity for the synthesis of oligosaccharides and glycoconjugates, and it is becoming increasingly feasible to produce complex carbohydrates in large scale, following enzymatic biosynthetic pathways.

A comparison of sugar indicators enables a universal high-throughput sugar-1-phosphate nucleotidyltransferase assay

“…Based upon the TrEMBL databank [10], more than 8,000 non-polymerase nucleotidyltransferases have been reported. Given the predominance of nucleotidyltransferase and NDP-sugars in nature, the study of nucleotidyltransferases remains an active area of research and continues to offer many exciting opportunities ranging from potential targets for the design of anti-infectives [11][12][13] to new tools for the synthesis of glycoconjugates [6,[14][15][16][17][18][19].…”