Enzymatic biofuel cells utilizing a biomimetic cofactor

Campbell, Elliot; Meredith, Matthew T.; Minteer, Shelley D.; Banta, Scott

doi:10.1039/c2cc16156g

Cited by 89 publications

(60 citation statements)

References 20 publications

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“…With respect to the second goal, the use of more stable thermoenzymes, better mediators and cofactors, and better formulation of enzymes can prolong the lifetime of EFCs to months or longer at room temperature, similar to the proteases used in liquid detergents. Moreover, costly and labile NAD can be replaced with low-cost and stable biomimics 41,42 .…”

Section: Discussionmentioning

confidence: 99%

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

247

194

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High-energy-density, green, safe batteries are highly desirable for meeting the rapidly growing needs of portable electronics. The incomplete oxidation of sugars mediated by one or a few enzymes in enzymatic fuel cells suffers from low energy densities and slow reaction rates. Here we show that nearly 24 electrons per glucose unit of maltodextrin can be produced through a synthetic catabolic pathway that comprises 13 enzymes in an air-breathing enzymatic fuel cell. This enzymatic fuel cell is based on non-immobilized enzymes that exhibit a maximum power output of 0.8 mW cm À 2 and a maximum current density of 6 mA cm À 2 , which are far higher than the values for systems based on immobilized enzymes. Enzymatic fuel cells containing a 15% (wt/v) maltodextrin solution have an energy-storage density of 596 Ah kg À 1 , which is one order of magnitude higher than that of lithium-ion batteries. Sugar-powered biobatteries could serve as next-generation green power sources, particularly for portable electronics.

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Section: Discussionmentioning

confidence: 99%

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

247

194

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“…A mutant with changes in three amino acids can work on NMN [135]. Recently, Scott et al demonstrated that engineered Pyrococcus furiosus alcohol dehydrogenase has an ability to work with NMN [59]. By following the same strategy, our lab has modified a thermophilic G6PDH that can work with NMN although its activity is very low (data not published).…”

Section: Redox Enzyme Engineeringmentioning

confidence: 86%

“…For example, both enzymatic pathways for hydrogen generation from polysaccharides and alcohol production from glucose do not need ATP for sugar phosphorylation, completely different from pathways occurring in microbes [20,24]. Another solution is the use of the low-cost biomimetic cofactors that have similar functions with natural cofactors [59][60][61]. Redox enzyme engineering has achieved the change of cofactor preference from one to another.…”

Section: Cell-free Biosystems For Biomanufacturing: Problems and Theimentioning

confidence: 97%

“…Further tuning of redox enzymes is needed due to its important application. Although the importance of redox enzyme engineering is recognized more and more to have a place in the future of biomanufacturing [9,104,145,146], redox enzyme engineering remains in its early stages because there is no framework or a general rule for engineering redox enzymes with nonnatural cofactors [59].…”

Section: Redox Enzyme Engineeringmentioning

confidence: 99%

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Cell-free Biosystems in the Production of Electricity and Bioenergy

Zhu

Tam²,

Zhang

2013

Fundamentals and Application of New Bioproduction Systems

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: Increasing needs of green energy and concerns of climate change are motivating intensive R&D efforts toward the low-cost production of electricity and bioenergy, such as hydrogen, alcohols, and jet fuel, from renewable sugars. Cell-free biosystems for biomanufacturing (CFB2) have been suggested as an emerging platform to replace mainstream microbial fermentation for the cost-effective production of some biocommodities. As compared to whole-cell factories, cell-free biosystems comprised of synthetic enzymatic pathways have numerous advantages, such as high product yield, fast reaction rate, broad reaction condition, easy process control and regulation, tolerance of toxic compound/product, and an unmatched capability of performing unnatural reactions. However, issues pertaining to high costs and low stabilities of enzymes and cofactors as well as compromised optimal conditions for different source enzymes need to be solved before cell-free biosystems are scaled up for biomanufacturing. Here, we review the current status of cell-free technology, update recent advances, and focus on its applications in the production of electricity and bioenergy.

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“…In 2010, Campbell et al used rational design to change the specificity of an ADH to have a higher affinity for nicotinamide mononucleotide (NMNH) rather than NADH, broadening the specificity for the cofactor [40]. By engineering the active site of an NADH-dependent ADH from Pyrococcus furiosus, Campbell et al were able to substitute NMN in place of NAD and improve BFC performance [41]. Although the OCP dropped from 0.642 to 0.593 V, the current density increased from 16.1 to 22.8 μA cm 2 , suggesting increased mass transfer for the minimal cofactor.…”

Section: Protein Engineeringmentioning

confidence: 99%