Enzymatic biodegradable micro‐reactors for therapeutic applications

Abstract

Poly(epsilon-caprolactone) is a well known biocompatible polymer, widely used as drug immobilization systems. In this work poly(epsilon-caprolactone) microparticles with average size between 5 and 25 microm have been prepared by O/W emulsion evaporation method. Inside the microparticles, we have encapsulated Glucose Oxidase with the aim of preparing micro-reactors for enzymatic therapy. These microparticles were structurally characterized and its enzymatic activity analyzed in order to improve the enzyme entrapment. Thus, at the optimum synthesis conditions the enzyme entrapped in the microparticles showed an enzymatic activity of (29.9 +/- 2.1)% comparing with the same amount of free enzyme. Moreover the microparticles maintained a (70.4 +/- 3.2)% of their initial enzymatic activity after placing them in buffer solution for two weeks.

Microchip CE‐LIF method for the hydrolysis of L‐glutamine by using L‐asparaginase enzyme reactor based on gold nanoparticle

“…In the past decades, enzyme immobilization methods have offered effective ways to overcome the limitations of free enzyme during enzymatic reaction, which provide strategies for obtaining rapid, highly sensitive, and high throughput digestion. A series of novel supports, such as silica , nanoparticles , capillaries , membranes , monoliths , and other surface anchored systems have been utilized in enzyme immobilization. Nowadays, exploration of the novel biocompatible surface for enzyme anchoring is an interesting topic for exerting good application of enzyme reactors in biosensing and biomedical field.…”