Enzymatic behavior of bovine liver catalase in aqueous medium of sugar based deep eutectic solvents

Ghobadi, Roohollah; Divsalar, Adeleh

doi:10.1016/j.molliq.2020.113207

Cited by 26 publications

(13 citation statements)

References 28 publications

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“…DESs have attracted the attention of the scientific community because of their potential applications. These mixtures can be used in organic synthesis, electrochemistry, extraction media [30][31][32][33][34], biotechnology or biodiesel [35][36][37] synthesis and separation processes [38,39], as a solvent in biological assays or enzymatic reactions [40], in biocatalysis [41,42], or in biomedical applications [43][44][45][46]. Additionally, they have been used in the pharmaceutical industry as excipients for increasing the solubility of hydrophobic drugs, or in drug delivery formulations [47][48][49][50][51].…”

Section: Deep Eutectic Solvents (Dess)mentioning

confidence: 99%

Deep Eutectic Solvents: Are They Safe?

et al. 2021

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Deep eutectic solvents (DESs) are a relatively new type of solvent that have attracted the attention of the scientific community due to their environmentally friendly properties and their versatility in many applications. Many possible DESs have been described and, thus, it is not easy to unequivocally characterize and generalize their properties. This is especially important in the case of the (eco)toxicity information that can be found for these mixtures. In this review, we collect data on the human and environmental toxicity of DESs, with the aim of gathering and exploring the behavioral patterns of DESs. The toxicity data found were analyzed attending to different factors: hydrogen bond donors or acceptors that form part of the eutectic mixture, pH, and the presence of organic acids in the DES molar ratio of the components, or interactions with natural compounds. In the case of ecotoxicity, results generally depend on the biomodel studied, along with other factors that have been also revised. Finally, we also carried out a revision of the biodegradation of DESs.

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Section: Deep Eutectic Solvents (Dess)mentioning

confidence: 99%

Deep Eutectic Solvents: Are They Safe?

et al. 2021

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“…In addition, DESs have a positive influence on HRP stability and enhance cytochrome C activity [ 58 , 59 ]. As a matter of fact, Ghobadi et al reported an increased binding affinity of H 2 O 2 to Bovine liver catalase when using a ChCl-based DES [ 60 ].…”

Section: Biocompatibility Of Dessmentioning

confidence: 99%

Deep Eutectic Solvents (DESs) and Their Application in Biosensor Development

Svigelj

Dossi

Grazioli

et al. 2021

Sensors

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Deep Eutectic Solvents (DESs) are a new class of solvents characterized by a remarkable decrease in melting point compared to those of the starting components. The eutectic mixtures can be simply prepared by mixing a Hydrogen Bond Acceptor (HBA) with a Hydrogen Bond Donor (HBD) at a temperature of about 80 °C. They have found applications in different research fields; for instance, they have been employed in organic synthesis, electrochemistry, and bio-catalysis, showing improved biodegradability and lower toxicity compared to other solvents. Herein, we review the use of DESs in biosensor development. We consider the emerging interest in different fields of this green class of solvents and the possibility of their use for the improvement of biosensor performance. We point out some promising examples of approaches for the assembly of biosensors exploiting their compelling characteristics. Furthermore, the extensive ability of DESs to solubilize a wide range of molecules provides the possibility to set up new devices, even for analytes that are usually insoluble and difficult to quantify.

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“…Kosmotropes and chaotropes would be nowadays more understood as a characterization of the degree of hydration, and different models are developed to consider the various effects observed experimentally [60]. Ionic liquids (IL) would follow the same tendency as salts where kosmotropic anions and chaotropic cations stabilize the enzyme, while chaotropic anions and kosmotropic cations destabilize it [61]. While they can be considered as eco-friendly solvents, many proteins are inherently inactive in ILs, requiring the addition of water for activity recovery.…”

Section: Salt Effectmentioning

confidence: 99%

“…While they can be considered as eco-friendly solvents, many proteins are inherently inactive in ILs, requiring the addition of water for activity recovery. This suggests ILs could affect the internal water shell [61,62].…”

Section: Salt Effectmentioning

confidence: 99%

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

et al. 2021

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Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing, electricity production, or biosynthesis of fine products. Despite advances in the knowledge of parameters that drive the efficiency of enzymatic electrocatalysis, the weak stability of bioelectrodes prevents large scale development of bioelectrocatalysis. In this review, starting from the understanding of the parameters that drive protein instability, we will discuss the main strategies available to improve all enzyme stability, including use of chemicals, protein engineering and immobilization. Considering in a second step the additional requirements for use of redox enzymes, we will evaluate how far these general strategies can be applied to bioelectrocatalysis.

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Enzymatic behavior of bovine liver catalase in aqueous medium of sugar based deep eutectic solvents

Cited by 26 publications

References 28 publications

Deep Eutectic Solvents: Are They Safe?

Deep Eutectic Solvents: Are They Safe?

Deep Eutectic Solvents (DESs) and Their Application in Biosensor Development

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

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