Enzymatic antioxidant defences are transcriptionally regulated in Es524, a copper-tolerant strain of <i>Ectocarpus siliculosus</i> (Ectocarpales, Phaeophyceae)

Sáez, Claudio A.; Ramesh, Kirti; Greco, Maria; Bitonti, Maria Beatrice

doi:10.2216/15-30.1

Cited by 13 publications

(8 citation statements)

References 33 publications

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“…On the other hand, it could be hypothesised that gene repression under copper and combined MAPK inhibitors is caused by the low levels of intracellular copper detected in these treatments in Celis-Plá et al [1]. Indeed, it has been observed that the expression of the enzymes SOD, CAT, and APX had a positive correlation with levels of intracellular copper in the brown macroalga Ectocarpus siliculosus [5,39]. However, this explanation appears unlikely considering that the decline of antioxidant defences in treatments with copper and combined MAPK inhibitors most of the time reached levels even below control conditions.…”

Section: Discussionmentioning

confidence: 99%

MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms

Rodríguez-Rojas

Celis-Plá

Méndez

et al. 2019

IJMS

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Following the physiological complementary/parallel Celis-Plá et al., by inhibiting extracellular signal regulated kinases (ERK), c-Jun N-terminal kinases (JNK), and cytokinin specific binding protein (p38), we assessed the role of the mitogen-activated protein kinases (MAPK) pathway in detoxification responses mediated by chronic copper (10 µM) in U. compressa. Parameters were taken at 6, 24, and 48 h, and 6 days (d). H2O2 and lipid peroxidation under copper and inhibition of ERK, JNK, or p38 alone increased but recovered by the sixth day. By blocking two or more MAPKs under copper, H2O2 and lipid peroxidation decayed even below controls. Inhibition of more than one MAPK (at 6 d) caused a decrease in total glutathione (reduced glutathione (GSH) + oxidised glutathione (GSSG)) and ascorbate (reduced ascorbate (ASC) + dehydroascorbate (DHA)), although in the latter it did not occur when the whole MAPK was blocked. Catalase (CAT), superoxide dismutase (SOD), thioredoxin (TRX) ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), and glutathione synthase (GS), were downregulated when blocking more than one MAPK pathway. When one MAPK pathway was blocked under copper, a recovery and even enhancement of detoxification mechanisms was observed, likely due to crosstalk within the MAPKs and/or other signalling processes. In contrast, when more than one MAPK pathway were blocked under copper, impairment of detoxification defences occurred, demonstrating that MAPKs were key signalling mechanisms for detoxification in macroalgae.

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Section: Discussionmentioning

confidence: 99%

MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms

Rodríguez-Rojas

Celis-Plá

Méndez

et al. 2019

IJMS

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“…The primers (forward and reverse) were designed from NIH GenBank using Primer Output 3 software (Table 2 ). Acanthamoeba actin related protein 2 (ARP2) is used as housekeeping gene in this study [ 42 , 43 ]. Quantitative polymerase chain reactions were performed using MyiQ cycler (Bio-Rad, Hercules, CA, USA) with iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression

Nordin

Ghafar

et al. 2017

Parasites Vectors

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BackgroundIn recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba.ResultsIn this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect.ConclusionsAll ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis.Electronic supplementary materialThe online version of this article (doi: 10.1186/s13071-017-2547-0) contains supplementary material, which is available to authorized users.

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“…Moreover, some algal species have been reported to display increased tolerance to copper due to a constant exposure to elevated copper concentrations. [48,60,61] Therefore, the use of unrealistically high copper concentrations in ecotoxicological studies can also have physiological relevance for the toxicity threshold of copper-tolerant marine algal species. [36] …”

Section: Discussionmentioning

confidence: 99%

Copper ecotoxicology of marine algae: a methodological appraisal

Leal

Hurd

Sander

et al. 2016

Chemistry and Ecology

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The production of accurate and reliable data on copper ecotoxicology of marine algae depends on the use of trace metal clean techniques during experimentation. We reviewed the methodologies used in the literature on copper ecotoxicology of marine macro-and microalgae, specifically the use of trace metal clean procedures such as the labware used (glassware vs. plasticware), methods of cleaning the labware (acid soaking and ultrapure water rinsing), stock solution preparation (copper source and acidification), and measurement and reporting of dissolved copper concentrations. In terms of taxonomic classification, the most studied algal groups were the Phyla Ochrophyta, Bacillariophyta, Rhodophyta, and Chlorophyta. In terms of methodology, ∼50% of the articles did not specify the labware, ∼25% used glassware, and ∼25% plasticware; ∼30% of the studies specified cleaning protocols for labware to remove trace metal impurities; the copper form used to prepare the stock solutions was specified in ∼80% of studies but acidification to stabilise the dissolved copper was performed in only ∼20%; and the dissolved copper concentration was measured in only ∼40% of studies. We discuss the importance of following trace metal clean techniques for the comparison and interpretation of data obtained on copper ecotoxicology in algae. ARTICLE HISTORY

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Enzymatic antioxidant defences are transcriptionally regulated in Es524, a copper-tolerant strain of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)

Cited by 13 publications

References 33 publications

MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms

MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms

Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression

Copper ecotoxicology of marine algae: a methodological appraisal

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