Enzymatic and computational analysis of large-ring cyclodextrin production by Corynebacterium glutamicum amylomaltase
Sirikul Ngawiset
Abstract:Amylomaltase is a well-known glucanotransferase enzyme that can produce large-ring cyclodextrins (LR-CDs) via cyclization reaction. To determine important amino acid residues involved in LR-CDs production of amylomaltase from Corynebacterium glutamicum (CgAM), mutations at CA I and CA II subdomains were introduced by site-directed mutagenesis. P228Y, E231Y, A413F and G417F CgAM mutants were constructed from CgAM recombinant plasmid. The optimum pH and temperature of WT and mutants were around pH 6.0 - 7.5 and … Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.