Enzymatic and Antimicrobial Activity of Biologically Active Samples from Aloe arborescens and Aloe barbadensis

Abstract

Recently, the use of Aloe species has become very widespread. These are extensively used as a nutraceutical in a variety of health care products and food supplements. In addition, the occurrence of the quickly adaptable microorganisms, particularly bacteria, which can develop resistance to antibiotics, is a major problem for public health, and therefore, it is necessary to search for new antimicrobials. In our study, the content of total phenols, proanthocyanidins, and proteins in fresh and lyophilized samples of A. arborescens and A. barbadensis and their ethanol extracts was investigated. Furthermore, enzymatic and antioxidant activity of samples were studied. Since antimicrobial activity of fresh samples was determined in our latest research, a more detailed study of antimicrobial effectiveness of A. arborescens and A. barbadensis (lyophilized, extracts) was performed. Ethanol extracts in particular contain higher concentrations of bioactive substances and show the topmost antioxidant activity. The novelty of the study refers to the observation of industrially important enzyme activities such as α-amylase, cellulase, lipase, peroxidase, protease, and transglutaminase in the samples as well as the microbial growth inhibition rates determination (MGIR) at different concentrations of added aloe samples. All samples inhibited the growth of all tested microbial cells. MIC90 for A. arborescens and A. barbadensis were also determined in case of B. cereus, P. aeruginosa, P. fluorescens, and S. aureus. The results of our study tend to give credence to the popular use of both aloes in medicine and in the cosmetic, food, and pharmaceutical industries.

Supercritical Fluid and Conventional Extractions of High Value-Added Compounds from Pomegranate Peels Waste: Production, Quantification and Antimicrobial Activity of Bioactive Constituents

“…Negative control was prepared by mixing MeOH with an MeOH solution of DPPH. The antioxidant activity of extracts was reported as a percentage of inhibition (I) relative to the reference solution [ 42 ]. All experiments were conducted in triplicates and results are reported as a mean ± SD.…”

“…For quantitative analysis of the antimicrobial activity of the analyzed sample, the broth microdilution method (BMM) was used [ 30 , 42 , 43 ]. Mueller–Hinton broth was used as a universal nutrient medium for all tested microorganisms.…”

Nano-enabled antimicrobial thin films: design and mechanism of action

“…Some antimicrobial enzymes like antimicrobial peptides are reported to be found in natural defensive (immune) system of wide range of organisms including plants. 109,110 Leitgeb et al, 111 for example, conrmed the presence of enzymes such as aamylase, cellulase, lipase, peroxidase, protease, and transglutaminase in the Aloe arborescens and Aloe barbadensis ethanolic extract. The obtained enzymatic fractions are reported to have microbial growth inhibition activity.…”

UJI EFEKTIVITAS HAND SANITIZER GEL ORGANIK BERBAHAN DASAR ARAK BALI TERHADAP Staphylococcus aureus

“…(Nalawade et al, 2015). Selain itu, pada formula HS-C juga terdapat bahan berupa aloe barbadensis leaf juice powder, produk turunan lidah buaya yang memiliki sifat antimikroba (Leitgeb et al, 2021).…”