Enzymatic analysis of riverine bacterioplankton production

Sinsabaugh, Robert L.; Findlay, Stuart; Franchini, Paula; Fischer, David

doi:10.4319/lo.1997.42.1.0029

Cited by 151 publications

(140 citation statements)

References 49 publications

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“…Jacobsen & Rai (1991 reported that LAP activity increased with the degree of lake eutrophication. Kroer et al (1994) found that specific LAP activities were similar among marine, estuarine and riverine bacterioplankton despite wide differences in the concentration of inorganic N. Similar observations were made by Sinsabaugh et al (1997) in a comparison of 3 large rivers. Muller-Niklas et al (1994) reported that cell-specific activities of LAP and other enzymes were similar for bacteria associated with marine snow and for those suspended in the surrounding seawater.…”

Section: Introductionsupporting

confidence: 66%

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Matric and particulate phosphatase and aminopeptidase activity in limnetic biofilms

Thompson¹,

Sinsabaugh²

2000

Aquat. Microb. Ecol.

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Biofdms are a major nexus for biogeochemical transformations in inland waters. A defining feature of biofdms is an extracellular polysaccharide matrix. One of the proposed functions of the matrix is retention of extracellular enzymes. We investigated this function by following the kinetics of alkaline phosphatase (AP) and leucine aminopeptidase (LAP) in shaded and exposed limnetic biofilms developed on glass slides. Organic matter (OM) accumulation and chlorophyll content were also measured. Biofilrn was scraped from the slides and partitioned in matric and particulate (ectocellular plus detrital bound) fractions. In addition to enzyme activity, the matric fraction was analyzed for carbohydrate and protein content. Over the May to October study period, matric K, values were sigmficantly higher (by 30 to 50%) than particulate values for both enzymes in both treatments (n = 44). V,,,,, values were significantly higher in the particulate fraction for both enzymes in both treatments. Matric activity as a fraction of total activity averaged about 25% (3:l ratio particulate: matric) for both enzymes, suggesting that the matrix was retaining enzymes. This was corroborated by principal component analysis which In general t~e d matric enzyme activity w~t h matric carbohydrate and protein content and with OM accumulation and linked phosphatase activity to chlorophyll. There was no correlation between matric and particulate enzyme activities and both varied widely. During periods of seasonal turnover in dominant algal populations, matric activity exceeded particulate. Matric enzymes appear to be a significant community resource whose activity may affect the dynamics of biofilm communities.

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Section: Introductionsupporting

confidence: 66%

“…Several studies have correlated peptidase activities with substrate availability, substrate uptake and bacterial productivity (e.g. Hoppe et al 1988, Miinster 1991, Muller-Niklas et al 1994, Sinsabaugh et al 1997, Foreman et al 1998). Jacobsen & Rai (1991 reported that LAP activity increased with the degree of lake eutrophication.…”

Section: Introductionmentioning

confidence: 99%

Matric and particulate phosphatase and aminopeptidase activity in limnetic biofilms

Thompson¹,

Sinsabaugh²

2000

Aquat. Microb. Ecol.

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“…Assays associated with the large bottle amendments were conducted at ambient laboratory temperature (ϳ20ЊC). Sodium bicarbonate (4 mmol L Ϫ1 ) was substituted for whole water in substrate controls, whereas quench standards were prepared using whole water and 1 mol L Ϫ1 of 4-methylumbelliferone (Sinsabaugh et al 1997). Enzyme activities were calculated using a reference standard containing 1 mol L Ϫ1 of 4-methylumbelliferone.…”

Section: Methodsmentioning

confidence: 99%

Phosphorus and trace metal limitation of algae and bacteria in Lake Superior

Sterner¹,

Smutka²,

McKay³

et al. 2004

Limnology & Oceanography

137

114

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We performed a set of nutrient-enrichment bioassay experiments using metal-clean protocols, testing for macronutrient and micronutrient limitation of algae and bacteria in western Lake Superior. Our analysis involved a novel approach to estimating the strength of nutrient limitation in factorial enrichment designs. We found that phytoplankton and bacterioplankton growth both increased with added phosphorus alone but not with iron, manganese, or zinc alone. However, even very small increases in growth rates following P supplementation (on the order of an additional 0.1 d Ϫ1 ) induced iron limitation in the phytoplankton, indicating that the algae in this large lake may be on the cusp of micronutrient and macronutrient limitation. In contrast, and contrary to expectations, bacteria responded only to P and not to metals. Spatial and temporal variability in degree and quality of nutrient limitation was considerable.

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“…phenol oxidase) represents an index of the quality of the dissolved organic C pool (LCI; Sinsabaugh and Shah 2011). All EEA assays used the microplate protocols originally developed by Sinsabaugh and colleagues (Sinsabaugh et al 1997;Sinsabaugh and Foreman 2001) and modified according to Hill et al (2010). Each microplate array included quadruplicate assays for each enzyme and each reference standard.…”

Section: Extracellular Enzymes Activities (Eea)mentioning

confidence: 99%

Effects of urban stream burial on organic matter dynamics and reach scale nitrate retention

et al. 2014

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Nitrogen (N) retention in streams is an important ecosystem service that may be affected by the widespread burial of streams in stormwater pipes in urban watersheds. We predicted that stream burial suppresses the capacity of streams to retain nitrate (NO 3 -) by eliminating primary production, reducing respiration rates and organic matter availability, and increasing specific discharge. We tested these predictions by measuring whole-stream NO 3 -removal rates using 15 -retention through a combination of hydrological and biological processes. The channel shape of two of the buried reaches increased specific discharge which enhanced NO 3 -transport from the channel, highlighting the relationship between urban infrastructure and ecosystem function. Uptake lengths in the buried reaches were further lengthened by low stream biological NO 3 -demand, as indicated by NO 3 -uptake velocities 17-fold lower than that of the open reaches. We also observed differences in the periphyton enzyme activity between reaches, indicating that the effects of burial cascade from the microbial to the ecosystem scale. Our results suggest that streamElectronic supplementary material The online version of this article

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Enzymatic analysis of riverine bacterioplankton production

Cited by 151 publications

References 49 publications

Matric and particulate phosphatase and aminopeptidase activity in limnetic biofilms

Matric and particulate phosphatase and aminopeptidase activity in limnetic biofilms

Phosphorus and trace metal limitation of algae and bacteria in Lake Superior

Effects of urban stream burial on organic matter dynamics and reach scale nitrate retention

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