Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae)

Scarpassa, Vera Margarete; Tadei, Wanderli Pedro

doi:10.1590/s0034-71082000000400002

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…albitarsis from the Brazilian Amazon and from Colombia revealed a high level of genetic divergence. The genetic difference found was 0.047 to 0.148, which is similar to that found among members of anopheline species complexes 20 .…”

Section: Species Complexessupporting

confidence: 81%

Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus

Tadei

Thatcher

2000

Rev. Inst. Med. trop. S. Paulo

203

205

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SUMMARYVarious species of Anopheles (Nyssorhynchus) were studied in the Amazon with the objective of determining their importance as malaria vectors. Of the 33 known Anopheles species occurring in the Amazon, only 9 were found to be infected with Plasmodium. The different species of this subgenus varied both in diversity and density in the collection areas. The populations showed a tendency towards lower density and diversity in virgin forest than in areas modified by human intervention.The principal vector, An. darlingi, is anthropophilic with a continuous activity cycle lasting the entire night but peaking at sunset and sunrise. These species (Nyssorhynchus) are peridomiciliary, entering houses to feed on blood and immediately leaving to settle on nearby vegetation.Anopheles nuneztovari proved to be zoophilic, crepuscular and peridomiciliary. These habits may change depending on a series of external factors, especially those related to human activity.There is a possibility that sibling species exist in the study area and they are being studied with reference to An. darlingi, An. albitarsis and An. nuneztovari. The present results do not suggest the existence of subpopulations of An. darlingi in the Brazilian Amazon.

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Section: Species Complexessupporting

confidence: 81%

Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus

Tadei

Thatcher

2000

Rev. Inst. Med. trop. S. Paulo

203

205

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“…These species have been described as existing sympatrically or as isolated populations in different regions. 32 Based on morphology of male genitalia, we have confirmed the individuals used in this study to belong to An. konderi.…”

Section: Discussionsupporting

confidence: 70%

Improved Molecular Technique for the Differentiation of Neotropical Anopheline Species

Matson

Rios

Chávez³

et al. 2008

The American Journal of Tropical Medicine and Hygiene

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We evaluated a PCR-RFLP of the ribosomal internal transcribed spacer 2 region (ITS2) to distinguish species of Anopheles commonly reported in the Amazon and validated this method using reared F1 offspring. The following species of Anopheles were used for molecular analysis: An. (Nys.) benarrochi, An. (Nys.) darlingi, An. (Nys.) nuneztovari, An. (Nys.) konderi, An. (Nys.) rangeli, and An. (Nys.) triannulatus sensu lato (s.l.). In addition, three species of the subgenus Anopheles, An. (Ano.) forattini, An. (Ano.) mattogrossensis, and An. (Ano.) peryassui were included for testing. Each of the nine species tested yielded diagnostic banding patterns. The PCR-RFLP method was successful in identifying all life stages including exuviae with small fractions of the sample. The assay is rapid and can be applied as an unbiased confirmatory method for identification of morphologic variants, disputed samples, imperfectly preserved specimens, and life stages from which taxonomic keys do not allow for definitive species determination.

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“…nuneztovari populations in Colombia and Venezuela compared to those in the Brazilian Amazonia. This proposal was confirmed by several morphological, chromosomal, molecular, behavioural and genetic flow studies (Fritz et al 1994, Hribar 1994, Linley et al 1996, Conn et al 1998, Tadei et al 1998, Scarpassa et al 1999, Scarpassa & Tadei 2000). Most recently, it was estimated that An.…”

Section: Primary Malaria Vector Speciesmentioning

confidence: 69%

“…The different behaviour patterns determined for this species have been related to the three cytotypes. Type A [ Anopheles ( Nyssorhynchus ) goelddi Rozeboom & Gabaldón], present in Brazil, is zoophilic and exophagic, while Types B and C, which are both found in Colombia and Venezuela, are predominately both anthropophilic and endophagic (Scarpassa & Tadei 2000). Endophagic-exophilic differences over the species’ geographic range influence the use of residual insecticides to control malaria.…”

Section: Primary Malaria Vector Speciesmentioning

confidence: 99%

Malaria vector species in Colombia: a review

Lerma¹,

Solarte

Giraldo-Calderón³

et al. 2011

Mem. Inst. Oswaldo Cruz

104

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Here we present a comprehensive review of the literature on the vectorial importance of the major Anopheles malaria vectors in Colombia. We provide basic information on the geographical distribution, altitudinal range, immature habitats, adult behaviour, feeding preferences and anthropophily, endophily and infectivity rates. We additionally review information on the life cycle, longevity and population fluctuation of Colombian Anopheles species. Emphasis was placed on the primary vectors that have been epidemiologically incriminated in malaria transmission: Anopheles darlingi, Anopheles albimanus and Anopheles nuneztovari. The role of a selection of local, regional or secondary vectors (e.g., Anopheles pseudopunctipennis and Anopheles neivai) is also discussed. We highlight the importance of combining biological, morphological and molecular data for the correct taxonomical determination of a given species, particularly for members of the species complexes. We likewise emphasise the importance of studying the bionomics of primary and secondary vectors along with an examination of the local conditions affecting the transmission of malaria. The presence and spread of the major vectors and the emergence of secondary species capable of transmitting human Plasmodia are of great interest. When selecting control measures, the anopheline diversity in the region must be considered. Variation in macroclimate conditions over a species’ geographical range must be well understood and targeted to plan effective control measures based on the population dynamics of the local Anopheles species.

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Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae)

Cited by 8 publications

References 19 publications

Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus

Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus

Improved Molecular Technique for the Differentiation of Neotropical Anopheline Species

Malaria vector species in Colombia: a review

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