Enzymatic alcoholysis of triolein to produce wax ester

Abstract

An alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. The effects of various reaction parameters such as time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/triolein), various organic solvents used and the initial water activity, a w of the reaction system were studied. The best conditions tested to produce wax ester were respectively, incubation time, 5 h; temperature, 50°C for Lipozyme and 60°C for Novozyme; weight of enzyme, 0.30 g and molar ratio of oleyl alcohol to triolein, 6:1. The use of organic solvents greatly in¯uenced the activity of lipase. Generally, the activity of lipase was high in nonpolar solvents with log P values greater than 2.50. Heptane and hexane were the best solvents tested. The enzymatic synthesis of oleyl oleate was best carried out at a w 0.32. Analysis of the yield of the products of the reaction at optimized reaction condition using Lipozyme showed that 75.66% oleyl oleate was produced.

Kinetics of Enzymatic Synthesis of Liquid Wax Ester from Oleic Acid and Oleyl Alcohol

“…It is important to note that the reaction in a reactor was in sealed condition and with no removal of water. Similar finding was also reported by Mat Hadzir et al 13) in the synthesis of wax ester by enzymatic alcoholysis reaction between triolein and oleyl alcohol. On the other hand, the experimental data of the reaction in large scale is superior to the calculated data after 30 min reaction time.…”

Oleochemical Industry Future through Biotechnology

“…Wax esters can be defined as industrial esters that are produced in low volumes but are highly priced with high profit margins 48 . Wax esters are long chain esters consist of long chain fatty acids with different alcohols These compounds have been used in many potential applications such as excellent wetting behavior at interface 49 and non greasy feeling when applied on skin surface. Wax esters are the key ingredients in cosmetics, pharmaceuticals, lubricants, plasticizers and other chemical industries 50 .…”

Optimization of Enzymatic Synthesis of Palm-based Kojic Acid Ester Using Response Surface Methodology

“…to a more condense mixture and contribute to the decreasing of reaction rate 20) . This report helps in explaining that the excess enzyme used did not contributed to increase the yield of the percent conversion.…”