Enzymatic activity of glucose oxidase encapsulated in transparent glass by the sol-gel method

Yamanaka, Stacey A.; Nishida, Fumito; Ellerby, Lisa M.; Nishida, Clinton R.; Dunn, Bruce; Valentine, Joan Selverstone; Zink, Jeffrey I.

doi:10.1021/cm00021a001

Cited by 194 publications

(101 citation statements)

References 2 publications

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“…The K m value obtained with the present glucose biosensors based on the CNT -titania -Nafion and titania -Nafion (without CNT) were found to be 10 mM and 8.4 mM, respectively. These K m values are much smaller than the values obtained with conventional glucose biosensors based on sol-gel silicate film (50 mM) [23] and screen-printed sol-gel silicate carbon network (38 mM) [24]. The small K m value in the present Fig.…”

Section: à2contrasting

confidence: 74%

Amperometric Glucose Biosensor Based on Glucose Oxidase Encapsulated in Carbon Nanotube–Titania–Nafion Composite Film on Platinized Glassy Carbon Electrode

et al. 2007

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A highly sensitive and selective glucose biosensor has been developed based on immobilization of glucose oxidase within mesoporous carbon nanotube -titania -Nafion composite film coated on a platinized glassy carbon electrode. Synergistic electrocatalytic activity of carbon nanotubes and electrodeposited platinum nanoparticles on electrode surface resulted in an efficient reduction of hydrogen peroxide, allowing the sensitive and selective quantitation of glucose by the direct reduction of enzymatically-liberated hydrogen peroxide at À 0.1 V versus Ag/AgCl (3 M NaCl) without a mediator. The present biosensor responded linearly to glucose in the wide concentration range from 5.0 Â 10 À5 to 5.0 Â 10 À3 M with a good sensitivity of 154 mA M À1 cm À2. Due to the mesoporous nature of CNT -titaniaNafion composite film, the present biosensor exhibited very fast response time within 2 s. In addition, the present biosensor did not show any interference from large excess of ascorbic acid and uric acid.

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Section: à2contrasting

confidence: 74%

Amperometric Glucose Biosensor Based on Glucose Oxidase Encapsulated in Carbon Nanotube–Titania–Nafion Composite Film on Platinized Glassy Carbon Electrode

et al. 2007

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“…In recent years, many amperometric biosensors were constructed by exploiting the sol-gel processes for immobilizing the enzymes or other biologically active compounds. Beforehand, several groups (Audebert et al [261], Avnir et al [234,262], Tatsu et al [263], and Yamanakana et al [237]) have employed optical or electro-chemical means for probing the activity of glucose oxidase (GOD) encapsulated within sol-gel matrices, demonstrating the interest of these biologically modified ceramics for glucose sensing. The GOD activity of the gel was first monitored by electrochemistry after its fixation on the tip of an oxygen electrode [263], or optically by exploiting the transparency of the thin sol-gel oxide matrices [234,240,262].…”

Section: Sol-gel-derived Organically Modified Silicates For Biosensorsmentioning

confidence: 99%

Analytical Applications of Silica-Modified Electrodes -A Comprehensive Review

Walcarius

1999

Electroanalysis

150

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This article aims to review the electroanalytical applications of chemically modified electrodes involving silica and silica-containing matrices. After a brief introduction to silica chemistry, the implication of pure and grafted silicas in electrochemistry, as well as that of the silica-based materials originating from the sol-gel technology, will be discussed. The interest of silica-modified electrodes (SiO 2 -MEs) with respect to electroanalysis will be pointed out by means of several examples. In the last five years, successful applications of SiO 2 -MEs have been achieved. Among them, silica grafted with inorganic films or organic moieties was successfully applied to the selective preconcentration or the electrocatalytic detection of various analytes. Also, the ability of the sol-gel chemistry to encapsulate enzymes within a silica matrix without preventing its activity was exploited in the development of new amperometric biosensors. These promising fields are thought to lead to significant advances in the near future, because of the explosive growth in both the synthesis of novel mesoporous organically-modified silica-based materials and the huge development of sol-gel chemistry in tailoring and construction of new modified electrodes.

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“…During the 1990s researchers realized that sol-gel methods could be used to immobilize a wide range of enzymes and other proteins, leading to materials which exhibit biochemical responses [10][11][12][13][14][15]. As long as the biomolecule was soluble in the aqueous or alcohol-based solvent, it could be incorporated within the pores of the inorganic matrix, and effectively impart its properties to the resulting solid [1,6,9].…”

Section: Introductionmentioning

confidence: 99%

Bio-hybrid materials for immunoassay-based sensing of cortisol

Fang

Zhou

Lan

et al. 2009

J Sol-Gel Sci Technol

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Sol-gel encapsulation has been used as the basis for detecting cortisol by an immunoassay approach. Previous research showed that antibodies immobilized in the pores of a sol-gel derived silica were able to bind cortisol and be used as an immunosensor. However, this approach was not effective when measuring cortisol levels in human serum because of interference from other fluorescence sources. The present paper describes a protocol which overcomes these limitations and enables sol-gel immunoassays to effectively measure cortisol in human serum over the physiological range of cortisol blood concentrations in an adult (2-28 lg/dL). The method involves a standard additions approach in which various amounts of cortisol are added to the serum. The cortisol concentration values obtained with our sol-gel immunoassay were typically within 10% of the values obtained by traditional analytical methods. The protocol presented here represents a significant contribution to sol-gel sensing and immunoassays in particular, because of the ability to detect an analyte in human serum. In addition, this work reports the first comparison between results from a sol-gel immunosensor and an alternative immuno-binding method for analyte detection.

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Enzymatic activity of glucose oxidase encapsulated in transparent glass by the sol-gel method

Cited by 194 publications

References 2 publications

Amperometric Glucose Biosensor Based on Glucose Oxidase Encapsulated in Carbon Nanotube–Titania–Nafion Composite Film on Platinized Glassy Carbon Electrode

Amperometric Glucose Biosensor Based on Glucose Oxidase Encapsulated in Carbon Nanotube–Titania–Nafion Composite Film on Platinized Glassy Carbon Electrode

Analytical Applications of Silica-Modified Electrodes -A Comprehensive Review

Bio-hybrid materials for immunoassay-based sensing of cortisol

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