Enzymatic activity of cholesterol oxidase immobilized onto polymer nanoparticles mediated by Congo red

Silva, Rubens A.; Carmona-Ribeiro, Ana Maria; Petri, Denise Freitas Siqueira

doi:10.1016/j.colsurfb.2013.03.024

Cited by 19 publications

(16 citation statements)

References 33 publications

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“…To the best of our knowledge, ChOx immobilization on chitosan functionalized magnetic nanoparticles has not yet been explored. Until now, the immobilization of ChOx has been performed on different carriers, such as NiFe 2 O 4 /CuO/FeO-chitosan nanocomposite [ 27 ], PS/PEG and PS/PEG/CR particles [ 28 ], and a cross-linked matrix of chitosan (Chi)– ionic liquid (IL) (1-butyl-3-methylimidazolium tetrafluoroborate, which was modified with the electrodeposition of Au particles onto thiol (–SH) functionalized multi-walled carbon nanotubes (MWNTs), or shorter versions (MWNT(SH)–Au/Chi–IL/) [ 29 ]. In the literature, the activity of the immobilized ChOx is not reported; however, the activity of the biosensor in terms of repeated use has been described.…”

Section: Introductionmentioning

confidence: 99%

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

2020

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In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the properties of the synthesized chitosan functionalized maghemite nanoparticles was conducted. Characterization was performed using scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Characterizations of size distribution were performed using dynamic light scattering (DLS) measurements and laser granulometry. A chitosan functionalization layer was confirmed using potentiometric titration on variously synthesized chitosan functionalized maghemite nanoparticles, which is important for further immobilization of bioactive compounds. Furthermore, after activation of chitosan functionalized maghemite nanoparticles with glutaraldehyde (GA) or pentaethylenehexamine (PEHA), immobilization studies of enzyme cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) were conducted. Factors influencing the immobilization of enzymes, such as type and concentration of activating reagent, mass ratio between carrier and enzyme, immobilization time and enzyme concentration, were investigated. Briefly, microparticles made using the chitosan suspension cross-linking process (MC2) proved to be the most suitable for obtaining the highest activity of immobilized enzyme, and nanoparticles functionalized with chitosan using the covalent binding method (MC3) could compete with MC2 for their applications.

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Section: Introductionmentioning

confidence: 99%

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

2020

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“…This biosensor exhibited a linear response to cholesterol in the range of 0.04-0.22mM with a detection limit of 34.6µM, apparent K m of 0.062mM with cholesterol and a high sensitivity of 9.02µA mM −1 . Polymeric biosensors created by cholesterol oxidase immobilization onto Polystyrene/PEG/Congo red particles yielded linear response in the cholesterol concentration range of 100 mg dL −1 to 300 mg dL −1 [104].…”

Section: Applications Of Immobilized Cholesterol Oxidasementioning

confidence: 99%

“…This group used extracts of Acacia salicina leaves in order to scavenge the free radicals such as H 2 O 2 liberated during the enzymatic reaction. Polymers such as Poly-Ethylene Glycol (PEG), decorated polystyrene (PS) nanoparticles along with Congo red have also been used to immobilize cholesterol oxidase [104]. APTES modified and glutaraldehyde cross-linked silica nanoparticles were used to conjugate cholesterol oxidase, subsequently used as a biosensor with 200s response time and detection limit of 4.2 mg/dL [105].…”

Section: Immobilization On Other Nanoparticlesmentioning

confidence: 99%

Immobilization of Cholesterol Oxidase: An Overview

Ghosh¹,

Ahmad²,

Khare³

2018

TOBIOTJ

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Background: Cholesterol oxidases are bacterial oxidases widely used commercially for their application in the detection of cholesterol in blood serum, clinical or food samples. Additionally, these enzymes find potential applications as an insecticide, synthesis of anti-fungal antibiotics and a biocatalyst to transform a number of sterol and non-sterol compounds. However, the soluble form of cholesterol oxidases are found to be less stable when applied at higher temperatures, broader pH range, and incur higher costs. These disadvantages can be overcome by immobilization on carrier matrices. Methods: This review focuses on the immobilization of cholesterol oxidases on various macro/micro matrices as well as nanoparticles and their potential applications. Selection of appropriate support matrix in enzyme immobilization is of extreme importance. Recently, nanomaterials have been used as a matrix for immobilization of enzyme due to their large surface area and small size. The bio-compatible length scales and surface chemistry of nanoparticles provide reusability, stability and enhanced performance characteristics for the enzyme-nanoconjugates. Conclusion: In this review, immobilization of cholesterol oxidase on nanomaterials and other matrices are discussed. Immobilization on nanomatrices has been observed to increase the stability and activity of enzymes. This enhances the applicability of cholesterol oxidases for various industrial and clinical applications such as in biosensors.

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“…Overall, modification of proteins (and peptides) by hydrophobic fatty acids residues or amphiphilic block copolymers has been recognized as a promising and relatively safe strategy to deliver proteins (and peptides) to the brain [155]. On the other hand, similarly to enzymes [156,157,158,159], some loss of activity may occur upon peptide immobilization [160,161]. C -terminal immobilization distinctly reduced the activity of potent antimicrobial sequences though the correlation between the antimicrobial activity and structural properties such as amphipathicity and the structure-related activity profile of the investigated peptides did not change with immobilization [161].…”

Section: Novel Formulations For Ampmentioning

confidence: 99%

Novel Formulations for Antimicrobial Peptides

Carmona-Ribeiro

Carrasco

2014

IJMS

114

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Peptides in general hold much promise as a major ingredient in novel supramolecular assemblies. They may become essential in vaccine design, antimicrobial chemotherapy, cancer immunotherapy, food preservation, organs transplants, design of novel materials for dentistry, formulations against diabetes and other important strategical applications. This review discusses how novel formulations may improve the therapeutic index of antimicrobial peptides by protecting their activity and improving their bioavailability. The diversity of novel formulations using lipids, liposomes, nanoparticles, polymers, micelles, etc., within the limits of nanotechnology may also provide novel applications going beyond antimicrobial chemotherapy.

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Enzymatic activity of cholesterol oxidase immobilized onto polymer nanoparticles mediated by Congo red

Cited by 19 publications

References 33 publications

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

Immobilization of Cholesterol Oxidase: An Overview

Novel Formulations for Antimicrobial Peptides

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