Enzymatic Activity and Pathogenicity of Entomopathogenic Fungi from Central and Southeastern Mexico to Diaphorina citri (Hemiptera: Psyllidae)

Cortéz-Madrigal, Hipólito; Sánchez-Saavedra, José Manuel; Díaz‐Godínez, Gerardo; Mora-Aguilera, Gustavo

doi:10.3958/059.039.0310

Cited by 15 publications

(9 citation statements)

References 26 publications

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“…Consequently, isolates of B. bassiana, and M. anisopliae were produced chitinase, lipase, and protease enzymes. The data showed that isolates differed in their chitinase, lipase, and protease enzyme activities that are attributed to their intraspecific and interspecific variability [ 28 ]. The three potential isolates, B. bassiana AAUMB-29 , B. bassiana AAUMFB-77, and M. anisopliae AAUDM-43 displayed a high level of chitinase, lipase, and protease activities.…”

Section: Discussionmentioning

confidence: 99%

Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

2022

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Objective This study was aimed to assess the enzymatic activity and pathogenicity potential of Beauveria bassiana and Metarhizium anisopliae against whiteflies in Ethiopia. Results The data showed that Beauveria bassiana AAUMB-29, AAUMFB-77, and AAUEB-59 generated the highest chitinase (EI = 3.41), lipase (EI = 4.45), and protease activities (EI = 5.44) respectively. The pathogenicity study of isolates on whitefly nymphs and adults indicated significant variation (P < 0.05) with mortality ranging from 71.67 to 98.33% and 60 to 100% against Bemisia tabaci and Trialeurodes vaporariorum nymphs respectively. The mortality of adults was between 58 and 94.27% against B. tabaci and 59.03 to 95.37% against T.vaporariorum. The result also showed that AAUMB-29, AAUMFB-77, and AAUDM-43 were the most virulent with LC50 values of 2.7 × 104, 5.3 × 104, and 5.4 × 104 conidia/ml against nymphs of B. tabaci, and with LC50 values 6.8 × 104, 8.2 × 104, and 7.2 × 104 conidia/ml against nymphs of T. vaporariorum, respectively. The B. bassiana AAUMB-29, B. bassiana AAUMFB-77, and M. anisopliae AAUDM-43 induced the highest whitefly mortality than other isolates. These isolates can be recommended for further tests under field conditions to fully realize their potential as effective biocontrol agents against whitefly pests in tomato.

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Section: Discussionmentioning

confidence: 99%

Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

2022

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“…Enzymes production studies developed on this fungus did not show significant correlation with its pathogenicity (Cortez-Madrigal et al, 2014;Quesada-Sojo & Rivera-Méndez, 2016;Pérez-González & Valdes-Gonzalez, 2017). However, it has not been determined whether the pathogenic metabolism relies on transposon or mycovirus elements that may play a role as observed by B. bassiana (Kotta-Loizou & Coutts, 2017;Pitaluga et al, 2020).…”

Section: Discussionmentioning

confidence: 89%

Mycelial compatibility, anastomosis, and nucleus numbers of eight MexicanHirsutella citriformisstrains isolated fromDiaphorina citri

Pérez-González

Gómez-Flores

Tamez‐Guerra

2021

PeerJ

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Background Among entomopathogenic fungi, H. citriformis has been recognized as potential biocontrol agent against the Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae). Nevertheless, this fungus is poorly characterized. Previous molecular studies have shown high sequence similarities among strains, but significant differences in Diaphorina citri virulence. Objective The aim of the present study was to determine mycelial compatibility and anastomosis, and nucleus numbers in mycelium and conidia of eight H. citriformis strains isolated from mycosed D. citri adults collected from several Mexican states. Methods Mycelial compatibility and anastomosis evaluation was performed after pairing strains, leading to 36 confrontations, and cultured in chlorate minimum medium to obtain mutants for vegetative compatibility group. Results Hypha or conidia nuclei were visualized with safranin-O and 3% KOH, and 0.05% trypan blue–lactophenol solution. H. citriformis strains showed compatibly and anastomosis events after confrontation. In addition, they showed one nucleus per conidium and mycelium section. It was not possible to obtain H. citriformis nit mutants from the chlorate concentrations tested. Conclusions To date, this is the first report demonstrating mycelial compatibility, anastomosis occurrence, and hyphae and conidia nuclei number among H. citriformis strains.

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“…In recent years, a large number of entomopathogenic fungi have been reported for the control of D. citri. Paecilomyces variotii, Hirsutella citriformis and Akanthomyces lecanii have the ability to infect D. citri [27][28][29][30]. In addition, B. bassiana and M. anisopliae, which have been commercially produced and applied, can also be used for the control of D. citri.…”

Section: Introductionmentioning

confidence: 99%

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Yan

Líu

Idrees

et al. 2022

JoF

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The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the most widespread and devastating pest species in citrus orchards and is the natural vector of the phloem-limited bacterium that causes Huanglongbing (HLB) disease. Thus, reducing the population of D. citri is an important means to prevent the spread of HLB disease. Due to the long-term use of chemical control, biological control has become the most promising strategy. In this study, a novel highly pathogenic fungal strain was isolated from naturally infected cadavers of adult D. citri. The species was identified as Aspergillus fijiensis using morphological identification and phylogenetic analysis and assigned the strain name GDIZM-1. Tests to detect aflatoxin B1 demonstrated that A. fijiensis GDIZM-1 is a non-aflatoxin B1 producer. The pathogenicity of the strain against D. citri was determined under laboratory and greenhouse conditions. The results of the laboratory study indicated that nymphs from the 1st to 5th instar and adults of D. citri were infected by A. fijiensis GDIZM-1. The mortality of nymphs and adults of D. citri caused by infection with A. fijiensis increased with the concentration of the conidial suspension and exposure time, and the median lethal concentration (LC50) and median lethal time (LT50) values gradually decreased. The mortality of D. citri for all instars was higher than 70%, with high pathogenicity at the 7th day post treatment with 1×108 conidia/mL. The results of the greenhouse pathogenicity tests showed that the survival of D. citri adults was 3.33% on the 14th day post-treatment with 1 × 108 conidia/mL, which was significantly lower than that after treatment with the Metarhizium anisopliae GDIZMMa-3 strain and sterile water. The results of the present study revealed that the isolate of A. fijiensis GDIZM-1 was effective against D. citri and it provides a basis for the development of a new microbial pesticide against D. citri after validation of these results in the field.

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Enzymatic Activity and Pathogenicity of Entomopathogenic Fungi from Central and Southeastern Mexico to Diaphorina citri (Hemiptera: Psyllidae)

Cited by 15 publications

References 26 publications

Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

Mycelial compatibility, anastomosis, and nucleus numbers of eight MexicanHirsutella citriformisstrains isolated fromDiaphorina citri

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

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