Background: Polygalacturonase is one kind of pectinases which hydrolyze the alpha-1,4 glycosidic bond between galacturonic acid residue. Polygalacturonase has been widely used in the fields of food, biofuel, and textile industries, in which thermostable polygalacturonase is often demanded at high temperatures of 50-60°C. Herein, we reported a thermostable polygalacturonase producing from Aspergillus fumigatus isolated from the pile fermentation of Pu'er tea in China. Results: The thermophilic polygalacturonase-producing strain was identified as A. fumigatus L45 on basis of its morphology, physicochemical properties, and 18S rDNA analysis. The crucial fermentation parameters affecting polygalacturonase activity were optimized by response surface methodology (RSM); the optimum fermentation parameters were the following: inoculums concentration of 0.07 % (v/v), fermentation time of 36 h, pH of 5.0, and temperature of 45°C. Under the optimized conditions, the highest polygalacturonase activity of 359.1 ± 10.1 U/mL was obtained. The polygalacturonase showed good thermostability and pH stability. The enzyme was activated by metal ions Zn 2+ and Mg