Enzootic nasal tumour of goats: demonstration of a type D-realated retrovirus in nasal fluids and tumours

Heras, M. De las; Sharp, JM; Jalón, J. A. García de; Dewar, P.

doi:10.1099/0022-1317-72-10-2533

Cited by 34 publications

(20 citation statements)

References 5 publications

(4 reference statements)

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“…These findings, therefore, provide further support for the notion that JSRV is the aetiological agent of SPA. Although most type D retroviruses appear not to be pathogenic, a simian type D retrovirus (SRV 2) has been associated aetiologically with retroperitoneal fibromatosis in macaques (Bryant et al, 1986) and the other sheep and goat type D-related viruses have been implicated in epithelial tumours (De las Heras et al, 1991Vitellozzi et al, 1993). Thus, in contrast to other retrovirus models involving leukaemogenic viruses, these sheep and goat retroviruses may represent unique models of oncogenic retroviruses associated with the development of naturally occurring epithelial carcinomas (Cremer & Gruber, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Retroviruses related to the type D viruses of primates have been demonstrated in the house shrew (Tsutsui et al, 1985;Aoyama et al, 1988), sheep He et al, 1992;De las Heras et al, 1993), cattle (York et al, 1989) and goats (De las Heras et al, 1991). Of these type D viruses, only the simian retroviruses and the small ruminant retroviruses have been associated with any disease (Marx & Lowenstine, 1987;Sharp, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…The small ruminant viruses have been associated with three contagious tumours, sheep pulmonary adenomatosis (syn. SPA, jaagsiekte, ovine pulmonary carcinoma) (Sharp & Angus, 1990), enzootic nasal tumour of sheep (De las Heras et al, 1993) and enzootic nasal tumour of goats (De las Heras et al, 1991), all of which arise from secretory epithelial cells in the respiratory tract.…”

Section: Introductionmentioning

confidence: 99%

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Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Palmarini

Dewar

Heras

et al. 1995

Journal of General Virology

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Sheep pulmonary adenomatosis (SPA) is a naturally occurring contagious lung tumour of sheep which has been associated aetiologically with a type D-and Brelated retrovirus (Jaagsiekte retrovirus; JSRV). To improve understanding of the aetio-pathogenesis of SPA, the distribution and the sites of JSRV replication in sheep with naturally or experimentally induced SPA or in unaffected controls were identified. New immunological reagents were produced and a blocking enzymelinked immunosorbent assay (B-ELISA) and an immunohistochemical technique for the detection of JSRV major capsid protein at the tissue and cellular levels were developed. JSRV was detected only in the respiratory tract of sheep affected by pulmonary adenomatosis and specifically in the transformed epithelial cells of the alveoli of SPA-affected sheep.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Palmarini

Dewar

Heras

et al. 1995

Journal of General Virology

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“…Nasal fluid from a goat with naturally occurring ENA was collected, clarified and concentrated as described previously (De las Heras et al, 1991b). ENTV-2 was purified by isopycnic centrifugation as described previously for JSRV (Herring et al, 1983).…”

Section: Methodsmentioning

confidence: 99%

Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution

Ortı́n

Cousens

Minguijón

et al. 2003

Journal of General Virology

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The complete genome sequence of a new isolate of enzootic nasal tumour virus (ENTV-2), associated with enzootic nasal adenocarcinoma (ENA) of goats, was determined. The genome exhibits a genetic organization characteristic of b-retroviruses. ENTV-2 is closely related to the retrovirus (ENTV-1) associated with enzootic adenocarcinoma of sheep, and to jaagsiekte retrovirus. The main sequence differences between these viruses reside in orfX, the U3 LTR, two small regions in gag and the transmembrane (TM) region of env. Sequence analysis of the TM region of env from several sheep and goats naturally affected by ENA suggested that ENTV-1 and ENTV-2 are distinct viruses rather than geographical variants. Although both viruses transform secretory epithelial cells of the ethmoid turbinate, the study of their tissue distribution using specific PCRs showed that ENTV-2 establishes a disseminated lymphoid infection whereas ENTV-1 is mainly confined to the tumour.

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“…The ovine genome contains 15 to 20 copies of endogenous retroviruses (enJSRVs) (3,24,25,36,57) that are highly related to two oncogenic exogenous betaretroviruses, Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV) (14,40). enJSRV RNAs are highly expressed in the epithelium of the uterus (38,48), while the exogenous pathogenic viruses JSRV and ENTV appear to have a strict tropism for secretory cells of the respiratory tract (16,36,37). Expression of enJSRV RNAs in the ovine uterus was initially identified by differential display PCR and PCR-based subtraction hybridization experiments (48).…”

mentioning

confidence: 99%

Expression of Endogenous Betaretroviruses in the Ovine Uterus: Effects of Neonatal Age, Estrous Cycle, Pregnancy, and Progesterone

Palmarini

Gray

Carpenter

et al. 2001

J Virol

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The ovine genome contains 15 to 20 copies of endogenous retroviruses (enJSRVs) highly related to the oncogenic jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus. enJSRVs are highly expressed in the endometrial lumenal epithelia (LE) and glandular epithelia (GE) of the ovine uterus. The effects of neonatal age, estrous cycle, pregnancy, and progesterone on expression of enJSRVs in the ovine uterus were determined. Expression of enJSRV RNAs was absent from the uterus of ewes at birth, but enJSRV RNAs were expressed specifically in the LE and developing GE from postnatal day (PND) 7 to PND 56. In adult ewes, enJSRV RNAs were detected only in the epithelia of the uterine endometrium, as well as epithelia of the oviduct, cervix, and vagina. In cyclic ewes, endometrial enJSRV RNA abundance was lowest on day 1, increased 12-fold between days 1 and 13, and then decreased to day 15. In pregnant ewes, levels of endometrial enJSRV RNAs were high on day 11, increased to day 13, and then decreased to day 19. In day 17 and 19 conceptuses, enJSRV RNAs were also detected in binucleate cells of the trophectoderm. Immunoreactive JSRV capsid and envelope proteins were detected in the endometrial LE and GE, as well as in the binucleate cells of the conceptus. In transfection assays utilizing ovine endometrial LE cells, progesterone increased transcriptional activity of several enJSRV long terminal repeats. Collectively, these results indicate that transcription of enJSRVs in the endometrial epithelia of the ovine uterus is increased by progesterone and might support a role for enJSRVs in conceptus-endometrium interactions during the peri-implantation period and early placental morphogenesis.

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Enzootic nasal tumour of goats: demonstration of a type D-realated retrovirus in nasal fluids and tumours

Cited by 34 publications

References 5 publications

Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution

Expression of Endogenous Betaretroviruses in the Ovine Uterus: Effects of Neonatal Age, Estrous Cycle, Pregnancy, and Progesterone

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