Environmentally Safe Mercury(II) Ions Aided Zero-Background and Ultrasensitive SERS Detection of Dipicolinic Acid

Bai, Xiangru; Zeng, Yi; Zhou, Xiaodong; Wang, Xiaohua; Hu, Jiming

doi:10.1021/acs.analchem.7b02172

Cited by 45 publications

(19 citation statements)

References 47 publications

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“…As indicated above, the content of this biomarker is up to 15% of the dry weight of spores and its content varies depending on the species, strain of spore-forming bacteria and sporulation conditions [38,39,42,48]…”

Section: Possibilities Of Rs In the Study Of Bacterial Sporesmentioning

confidence: 99%

“…Significant differences in the Raman spectra of vegetative and spore cell forms of bacteria were discovered long ago in the study of Bacillus spp. These differences are associated with the predominance in the resonance spectra of bacterial endospores of Calcium Dipicolinate (Ca-DPA), the major peaks of which were detected at vibrational frequencies of 1015-1017 (cm -1 ) at an excitation wavelength of 244 nm [19,42,43,48,137].…”

Section: Possibilities Of Rs In the Study Of Bacterial Sporesmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Spores: Mechanisms of Stability and Targets for Modern Biotechnologies

In³

et al. 2019

BJSTR

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Some gram-positive bacteria of the genus Bacillus spp. and Clostridium spp. can exist in two alternative states: vegetative and in the form of endospores, which are divided into two types: having an outer shell (exosporium) and not having it. Bacterial controversies have been studied for a long time and yet they remain not fully recognized as objects of the microworld. These resting (dormant) cells, recognized as the most stable form of life on Earth, are formed in the mother cell with a lack of nutrients. The extraordinary resistance of spores to extreme physical and chemical conditions of existence is the main distinguishing feature from vegetative forms, and their life cycle contributes to the wide spread of spore-forming bacteria in various ecosystems. The unique properties of bacterial spores cause increased scientific and medical interest associated with the epidemiological significance of resting cell forms that can be in a metabolically inactive state for tens and hundreds of years. As soon as spores get into human or animal organisms, as well as into canned foods, they germinate and become a source of serious infectious diseases. Spores are an infectious form of spore-forming bacteria, among which the most dangerous are B. anthracis (anthrax causative agent), B. cereus (toxicoinfection), as well as representatives of the genus Clostridium -C. botulinum (botulism), C. perfringens (gas gangrene), and C. difficile (nosocomial infections). The relevance of the research and the prospectiveness of the study of spores mediates the need to disclose their biochemical structure, molecular mechanisms of steadiness, as well as detection and indication using modern analytical tools. The characterized supramolecular structures of endo-and exospores become a target for modern biotechnologies on the principle of "learning from Nature". These biological systems are perspective models for packaging and targeted delivery of enzymes, nucleic acids, antigens and drugs to specific tissues, cells and even intracellular organelles for the treatment of cancerous tumors and the creation of thermostable vaccines. The unique properties of bacterial spores will soon find application in ecosystem technologies as biofungicides and bioinsecticides in

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Section: Possibilities Of Rs In the Study Of Bacterial Sporesmentioning

confidence: 99%

Section: Possibilities Of Rs In the Study Of Bacterial Sporesmentioning

confidence: 99%

Bacterial Spores: Mechanisms of Stability and Targets for Modern Biotechnologies

In³

et al. 2019

BJSTR

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“…Compared with the existing DPA determination method, the accuracy and reliability of the determination are greatly improved. [83] Using short peptides to bind to the cholera toxin as a recognition element, the selective detection of cholera toxin based on SERS was achieved, with the LOD of 3.51 pg mL −1 . The sensitivity and specificity were much greater than those previously reported.…”

Section: Detection Signals Originating From Bacterial Metabolitesmentioning

confidence: 99%

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

et al. 2020

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The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface-enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be used to directly obtain molecular fingerprint information, as well as for the on-line qualitative analysis of multicomponent samples. It has therefore become an effective technique for bacterial detection. Within this progress report, advances in the detection of bacteria using SERS and other compatible techniques are discussed in order to summarize its development in recent years. First, the enhancement principle and mechanism of SERS technology are briefly overviewed. The second part is devoted to a label-free strategy for the detection of bacterial cells and bacterial metabolites. In this section, important considerations that must be made to improve bacterial SERS signals are discussed. Then, the label-based SERS strategy involves the design strategy of SERS tags, the immunomagnetic separation of SERS tags, and the capture of bacteria from solution and dye-labeled SERS primers. In the third part, several novel SERS compatible technologies and applications in clinical and food safety are introduced. In the final part, the results achieved are summarized and future perspectives are proposed.

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“…Significant differences in the spectra of the Raman scattering for the vegetative and spore cellular forms of bacteria were detected relatively long ago investigating Bacillus spp . These differences are related to the predominance of calcium dipicolinate in the resonance spectrum of the bacterial endospores whose major peaks were found at the oscillation frequencies of 1015–1017 cm –1 and 244 nm excitation wavelength [ 22 , 43 , 44 , 47 , 133 ]. As pointed above, the content of this marker amounted on average to 10% of a dry spore mass and vary dependent on the species, spore-forming bacterial strain, and sporulation conditions [ 37 , 40 , 43 , 47 ].…”

Section: Modern Technologies For Identification and Investigations Of Sporesmentioning

confidence: 99%

Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review)

Andryukov

Karpenko

Lyapun³

2020

Sovrem Tehnol Med

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The capability of some representatives of Clostridium spp . and Bacillus spp . genera to form spores in extreme external conditions long ago became a subject of medico-biological investigations. Bacterial spores represent dormant cellular forms of gram-positive bacteria possessing a high potential of stability and the capability to endure extreme conditions of their habitat. Owing to these properties, bacterial spores are recognized as the most stable systems on the planet, and spore-forming microorganisms became widely spread in various ecosystems. Spore-forming bacteria have been attracted increased interest for years due to their epidemiological danger. Bacterial spores may be in the quiescent state for dozens or hundreds of years but after they appear in the favorable conditions of a human or animal organism, they turn into vegetative forms causing an infectious process. The greatest threat among the pathogenic spore-forming bacteria is posed by the causative agents of anthrax ( B. anthracis ), food toxicoinfection ( B. cereus ), pseudomembranous colitis ( C. difficile ), botulism ( C. botulinum ), gas gangrene ( C. perfringens ). For the effective prevention of severe infectious diseases first of all it is necessary to study the molecular structure of bacterial spores and the biochemical mechanisms of sporulation and to develop innovative methods of detection and disinfection of dormant cells. There is another side of the problem: the necessity to investigate exo- and endospores from the standpoint of obtaining similar artificially synthesized models in order to use them in the latest medical technologies for the development of thermostable vaccines, delivery of biologically active substances to the tissues and intracellular structures. In recent years, bacterial spores have become an interesting object for the exploration from the point of view of a new paradigm of unicellular microbiology in order to study microbial heterogeneity by means of the modern analytical tools.

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Environmentally Safe Mercury(II) Ions Aided Zero-Background and Ultrasensitive SERS Detection of Dipicolinic Acid

Cited by 45 publications

References 47 publications

Bacterial Spores: Mechanisms of Stability and Targets for Modern Biotechnologies

Bacterial Spores: Mechanisms of Stability and Targets for Modern Biotechnologies

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review)

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