Environmental Transcription of
            <i>mmoX</i>
            by Methane-Oxidizing Proteobacteria in a Subarctic Palsa Peatland

Liebner, Susanne; Svenning, Mette M.

doi:10.1128/aem.02292-12

Cited by 52 publications

(57 citation statements)

References 33 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the diazotrophs, aerobic methanotrophs have been suggested to significantly contribute to the N pool in Northern peatlands (up to 40% of total N 2 fixation; Larmola et al 2014), which was consistent with the detection of an order of magnitude higher methanotroph nifH (a subunit of the gene encoding for the nitrogenase enzyme) expression as compared to cyanobacterial nifH expression (Vile et al 2014). In contrast, only a minor fraction of the total nifH sequences detected were of methanotrophic origin in a survey of Sphagnum mosses in alpine bogs (Bragina et al 2013) and a subartic palsa peatland (Liebner and Svenning 2013). Methanotrophmediated N 2 fixation also occurs in other environments (e.g., grassland soil; Buckley et al 2008: rice roots;Bao et al 2014), but their relative contribution to the total N 2 fixation in these habitats is unclear.…”

supporting

confidence: 74%

Diazotrophic methanotrophs in peatlands: the missing link?

Bodelier

2015

Plant Soil

View full text Add to dashboard Cite

supporting

confidence: 74%

Diazotrophic methanotrophs in peatlands: the missing link?

Bodelier

2015

Plant Soil

View full text Add to dashboard Cite

“…The significant correlation between methane oxidation and type II pmoA gene abundance, rather than the total pmoA gene abundance, suggests the active role of type II methanotrophs under the incubation conditions. Alphaproteobacterial methanotrophs belonging to the Methylocystis-Methylosinus group, as well as the genera Methylocella, Methylocapsa, and Methyloferula, seemingly form the predominantly active community in many acidic peat environments (25,27,47), although the presence of active gammaproteobacterial methanotrophs cannot be excluded (32,48).…”

Section: Discussionmentioning

confidence: 99%

“…The mmoX clone libraries were constructed from the DNA extract of all peat material sampled in 2015 using the primer pair mmoX-206F/mmoX-886R, with the primer concentrations and PCR thermal profile as shown in Table 2, according to Liebner and Svenning (27). The PCR mixture consisted of 25 l of MasterAmp 2ϫ PCR premix F (Epicentre, Illumina, WI, USA), 5 l of bovine serum albumin (BSA; 5 mg · ml Ϫ1 ; Invitrogen, Breda, the Netherlands), 0.5 l of each primer, 0.5 l of Taq polymerase (Thermo Fisher Scientific, Uden, the Netherlands), 1 l of template DNA, and 17.5 l of DNase-and RNase-free water (Sigma-Aldrich Chemie NV, Zwijndrecht, the Netherlands), giving a final volume of 50 l. The PCR amplicon was verified on 1% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

“…Because of the oligotrophic conditions in ombrotrophic peat, many of these microorganisms are slow-growing and resist isolation in the laboratory. To circumvent cultivation, quantitative and qualitative culture-independent approaches targeting the structural genes of methanogens (i.e., mcrA encoding the ␣-subunit of the methyl-coenzyme M reductase) and methanotrophs (i.e., pmoA encoding the A subunit of the particulate methane monooxygenase, and mmoX encoding the ␣-subunit of the soluble methane monooxygenase) have been employed to characterize peatinhabiting methane-cycling microbial communities (20,(25)(26)(27).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Impact of Peat Mining and Restoration on Methane Turnover Potential and Methane-Cycling Microorganisms in a Northern Bog

Reumer

Harnisz

Lee

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

Ombrotrophic peatlands are a recognized global carbon reservoir. Without restoration and peat regrowth, harvested peatlands are dramatically altered, impairing their carbon sink function, with consequences for methane turnover. Previous studies determined the impact of commercial mining on the physicochemical properties of peat and the effects on methane turnover. However, the response of the underlying microbial communities catalyzing methane production and oxidation have so far received little attention. We hypothesize that with the return of Sphagnum spp. postharvest, methane turnover potential and the corresponding microbial communities will converge in a natural and restored peatland. To address our hypothesis, we determined the potential methane production and oxidation rates in natural (as a reference), actively mined, abandoned, and restored peatlands over two consecutive years. In all sites, the methanogenic and methanotrophic population sizes were enumerated using quantitative PCR (qPCR) assays targeting the mcrA and pmoA genes, respectively. Shifts in the community composition were determined using Illumina MiSeq sequencing of the mcrA gene and a pmoA-based terminal restriction fragment length polymorphism (t-RFLP) analysis, complemented by cloning and sequence analysis of the mmoX gene. Peat mining adversely affected methane turnover potential, but the rates recovered in the restored site. The recovery in potential activity was reflected in the methanogenic and methanotrophic abundances. However, the microbial community composition was altered, being more pronounced for the methanotrophs. Overall, we observed a lag between the recovery of the methanogenic/methanotrophic activity and the return of the corresponding microbial communities, suggesting that a longer duration (Ͼ15 years) is needed to reverse mining-induced effects on the methane-cycling microbial communities.IMPORTANCE Ombrotrophic peatlands are a crucial carbon sink, but this environment is also a source of methane, an important greenhouse gas. Methane emission in peatlands is regulated by methane production and oxidation catalyzed by methanogens and methanotrophs, respectively. Methane-cycling microbial communities have been documented in natural peatlands. However, less is known of their response to peat mining and of the recovery of the community after restoration. Mining exerts an adverse impact on potential methane production and oxidation rates and on methanogenic and methanotrophic population abundances. Peat mining also induced a shift in the methane-cycling microbial community composition. Nev-

show abstract

“…Methane monooxygenase (MMO) is the key enzyme in methane oxidation, existing as soluble (sMMO) and particulate (pMMO) forms. The pmoA and mmoX genes encode subunits of pMMO and sMMO, respectively, and have been used to examine methanotroph diversity in culture-independent studies (9,10). Some methanotrophs have a particularly high affinity for methane and can oxidize methane at low (Յ40 ppm by volume [ppm v ]) to atmospheric (1.7 ppm v ) concentrations (11)(12)(13)(14).…”

mentioning

confidence: 99%

Termites Facilitate Methane Oxidation and Shape the Methanotrophic Community

Erens

Mujinya

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

g Termite-derived methane contributes 3 to 4% to the total methane budget globally. Termites are not known to harbor methaneoxidizing microorganisms (methanotrophs). However, a considerable fraction of the methane produced can be consumed by methanotrophs that inhabit the mound material, yet the methanotroph ecology in these environments is virtually unknown. The potential for methane oxidation was determined using slurry incubations under conditions with high (12%) and in situ (ϳ0.004%) methane concentrations through a vertical profile of a termite (Macrotermes falciger) mound and a reference soil. Interestingly, the mound material showed higher methanotrophic activity. The methanotroph community structure was determined by means of a pmoA-based diagnostic microarray. Although the methanotrophs in the mound were derived from populations in the reference soil, it appears that termite activity selected for a distinct community. Applying an indicator species analysis revealed that putative atmospheric methane oxidizers (high-indicator-value probes specific for the JR3 cluster) were indicative of the active nest area, whereas methanotrophs belonging to both type I and type II were indicative of the reference soil. We conclude that termites modify their environment, resulting in higher methane oxidation and selecting and/or enriching for a distinct methanotroph population.

show abstract

Environmental Transcription of mmoX by Methane-Oxidizing Proteobacteria in a Subarctic Palsa Peatland

Cited by 52 publications

References 33 publications

Diazotrophic methanotrophs in peatlands: the missing link?

Diazotrophic methanotrophs in peatlands: the missing link?

Impact of Peat Mining and Restoration on Methane Turnover Potential and Methane-Cycling Microorganisms in a Northern Bog

Termites Facilitate Methane Oxidation and Shape the Methanotrophic Community

Contact Info

Product

Resources

About