Environmental Surveillance of Enteroviruses in Central Argentina: First Detection and Evolutionary Analyses of E14

Farías, Adrián; Mojsiejczuk, Laura; Pisano, María Belén; Flores, Fernando S.; Aguilar, Javier; Jean, Ana N.; Yanes, Laura A.; Masachessi, Gisela; Prez, Verónica Emilse; Isa, Marı́a Beatrı́z; Campos, Rodolfo Héctor; Ré, Viviana Elizabeth; Nates, Silvia

doi:10.1007/s12560-017-9318-0

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…Additionally, there are some reports regarding outbreaks of different diseases caused by EVs [14][15][16][17]. Previous wastewater-based epidemiology studies have provided valuable information about EVs types circulating in Argentina [18][19][20][21], thus complementing clinical analysis of samples from patients with diseases associated with EVs reported in the 1990s and early 2000s [22,23]. Most of these environmental studies were carried out in the Province of Córdoba, but they were biased towards poliovirus isolation and viral strains characterization [19] or were based on Sanger sequencing of a PCR product obtained directly from the wastewater specimens [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…Previous wastewater-based epidemiology studies have provided valuable information about EVs types circulating in Argentina [18][19][20][21], thus complementing clinical analysis of samples from patients with diseases associated with EVs reported in the 1990s and early 2000s [22,23]. Most of these environmental studies were carried out in the Province of Córdoba, but they were biased towards poliovirus isolation and viral strains characterization [19] or were based on Sanger sequencing of a PCR product obtained directly from the wastewater specimens [20,21]. Considering that wastewater is a complex sample in which there normally occurs a mixture of several EVs types as a result of multiple excretion events in the population [24,25], and due to the inherent diversity of types when EVs are assessed, these approaches had limitations for providing a wide understanding on the diversity of circulating EVs.…”

Section: Introductionmentioning

confidence: 99%

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Environmental Surveillance through Next-Generation Sequencing to Unveil the Diversity of Human Enteroviruses beyond the Reported Clinical Cases

Lizasoain

Mir

Masachessi

et al. 2021

Viruses

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The knowledge about circulation of Human Enteroviruses (EVs) obtained through medical diagnosis in Argentina is scarce. Wastewater samples monthly collected in Córdoba, Argentina during 2011–2012, and then in 2017–2018 were retrospectively studied to assess the diversity of EVs in the community. Partial VP1 gene was amplified by PCR from wastewater concentrates, and amplicons were subject of next-generation sequencing and genetic analyses. There were 41 EVs detected, from which ~50% had not been previously reported in Argentina. Most of the characterized EVs (60%) were detected at both sampling periods, with similar values of intratype nucleotide diversity. Exceptions were enterovirus A71, coxsackievirus B4, echovirus 14, and echovirus 30, which diversified in 2017–2018. There was a predominance of types from EV-C in 2017–2018, evidencing a common circulation of these types throughout the year in the community. Interestingly, high genetic similarity was evidenced among environmental strains of echovirus 30 circulating in 2011–2012 and co-temporal isolates obtained from patients suffering aseptic meningitis in different locations of Argentina. This study provides an updated insight about EVs circulating in an important region of South America, and suggests a valuable role of wastewater-based epidemiology in predicting outbreaks before the onset of cases in the community.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Environmental Surveillance through Next-Generation Sequencing to Unveil the Diversity of Human Enteroviruses beyond the Reported Clinical Cases

Lizasoain

Mir

Masachessi

et al. 2021

Viruses

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“…Enteroviruses are responsible for several diseases that adversely impact public health, notably neurological manifestations: lymphocytic meningitis and flaccid paralysis (EV-D70 and EV-A71) 24,25 skin and mucosal membrane manifestations: hemorrhagic conjunctivitis, MPB syndrome (EV-A71) 26 , and rhinopharyngitis and respiratory infections: bronchiolitis (EV-D68). 27 The EV capsid VP1 protein is one of four structural proteins of EV.…”

Section: Discussionmentioning

confidence: 99%

Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71

Boussettine¹,

Kasmi²,

Hassou³

et al. 2021

J. Pure Appl. Microbiol.

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The three human Enterovirus serotypes D-68, D-70, and A-71, are common pathogens that are transmitted by fecal-oral and aerosol routes. These positive RNA viruses were known to exhibit high levels of genetic diversity and variability. Currently, no vaccines are available to protect humans from these three serotypes. Therefore, efforts are needed for the development of a vaccine directed against heterologous viruses. In our study, an immunoinformatics approach is used to identify T- and B-cell epitopes that may help for the generation of a universal vaccine against EV-D70, EV-A71, and EV-D68. B and T cell epitopes were selected based on their length. As a result, 5 B cell epitopes and 18 T cell epitopes were predicted. Our B cell epitope prediction results showed that there are a number of linear regions. Position 150-170 was found to be the most immunogenic for the different strains. Regarding the epitopes of the T lymphocytes, the result of the interactions shows that 95% of the predicted epitopes are common between the 3 sequences and the 5 methods used. These results demonstrate the great immunogenic potential of these sequences and their capacities to trigger immune reactions in people with different HLA alleles. The “VFYDGFAGF” epitope is the most important and most immunogenic for triggering an immune response. Our study results allowed us to identify epitopes to be used in the development of cross-protection vaccines against the three Enterovirus serotypes. However, in vivo and in vitro studies are needed to assess the potential of the epitopes predicted by our study.

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“…Some articles assessed the presence of poliovirus, [69][70][71][72] either wildtype or vaccine-derived, including potential neurovirulence of vaccine-derived poliovirus. [73][74][75] Many articles documented the diversity of non-polio enteroviruses found in wastewater, 73,[76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92] with a variety of focuses including rotavirus, [93][94][95][96][97] norovirus, 88,92,[98][99][100] astrovirus, 101,102 polyomavirus, 103 Saffold virus, 104 hepatitis A virus, 85 hepatitis E virus, 105 mastadenovirus, 106 Aichi virus, 107 and human bocavirus. 107 Surveillance of Giardia and/or Cryptosporidium was also documented.…”

Section: Figure 2 Global Distribution Of Studies Of Wastewater Surveillance For Infectious Diseasementioning

confidence: 99%

Wastewater surveillance for infectious disease: a systematic review

Kilaru

Hill

Anderson³

et al. 2021

Preprint

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Wastewater surveillance of SARS-CoV-2 has shown to be a valuable source of information regarding SARS-CoV-2 transmission and COVID-19 cases. Though the method has been used for several decades to track other infectious diseases, there has not been a comprehensive review outlining all of the pathogens surveilled through wastewater. The aim of this study is to identify what infectious diseases have been previously studied via wastewater surveillance prior to the COVID-19 Pandemic and identify common characteristics between the studies, as well as identify current gaps in knowledge. Peer-reviewed articles published as of August 1, 2020 that examined wastewater for communicable and infectious human pathogens on 2 or more occasions were included in the study. Excluded from this list were all reviews and methods papers, single collection studies, and non-human pathogens. Infectious diseases and pathogens were identified in studies of wastewater surveillance, as well as themes of how wastewater surveillance and other measures of disease transmission were linked. This review did not include any numerical data from individual studies and thus no statistical analysis was done. 1005 articles were identified but only 100 were included in this review after applying the inclusion criteria. These studies came from 38 countries with concentration in certain countries including Italy, Israel, Brazil, Japan, and China. Twenty-five separate pathogen families were identified in the included studies, with the majority of studies examining pathogens from the family Picornaviridae, including polio and non-polio enteroviruses. Most studies of wastewater surveillance did not link what was found in the wastewater to other measures of disease transmission. Among those studies that did compare wastewater surveillance to other measures of disease transmission the value observed was dependent upon pathogen and varied by study. Wastewater surveillance has historically been used to assess water-borne and fecal-orally transmitted pathogens causing diarrheal disease. However, numerous other types of pathogens have been surveilled using wastewater and wastewater surveillance should be considered as a potential tool for many infectious diseases. Wastewater surveillance studies can be improved by incorporating other measures of disease transmission at the population-level including disease incidence and hospitalizations.

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Environmental Surveillance of Enteroviruses in Central Argentina: First Detection and Evolutionary Analyses of E14

Cited by 8 publications

References 18 publications

Environmental Surveillance through Next-Generation Sequencing to Unveil the Diversity of Human Enteroviruses beyond the Reported Clinical Cases

Environmental Surveillance through Next-Generation Sequencing to Unveil the Diversity of Human Enteroviruses beyond the Reported Clinical Cases

Computational Approach for Predicting Common Epitopes in the VP1 Structural Protein of Enterovirus Serotypes EV-D68, EV-D70, and EV-A71

Wastewater surveillance for infectious disease: a systematic review

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