Environmental signals implicated in Dr fimbriae release by pathogenic Escherichia coli

Diard, Stéphane; Toribio, Ana Luisa; Boum, Yap; Vigier, Florence; Kansau, Imad; Bouvet, Odile; Servin, Alain L.

doi:10.1016/j.micinf.2006.02.023

Cited by 11 publications

(15 citation statements)

References 30 publications

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“…Recently, it has been reported that the AfaD adhesin also has invasin properties by specifically binding to integrin a5b1 [44]. A recent study by Diard et al [45] has shown that Dr fimbriae can be released into the extracellular medium in response to environmental signals, such as temperature and reduced oxygen, but the purpose of this mechanism remains elusive.…”

Section: Pili Of Gram-negative Bacteriamentioning

confidence: 99%

Pili in Gram-negative and Gram-positive bacteria — structure, assembly and their role in disease

Proft

Baker

2008

Cell. Mol. Life Sci.

449

406

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Many bacterial species possess long filamentous structures known as pili or fimbriae extending from their surfaces. Despite the diversity in pilus structure and biogenesis, pili in Gram-negative bacteria are typically formed by non-covalent homopolymerization of major pilus subunit proteins (pilins), which generates the pilus shaft. Additional pilins may be added to the fiber and often function as host cell adhesins. Some pili are also involved in biofilm formation, phage transduction, DNA uptake and a special form of bacterial cell movement, known as 'twitching motility'. In contrast, the more recently discovered pili in Gram-positive bacteria are formed by covalent polymerization of pilin subunits in a process that requires a dedicated sortase enzyme. Minor pilins are added to the fiber and play a major role in host cell colonization.This review gives an overview of the structure, assembly and function of the best-characterized pili of both Gram-negative and Gram-positive bacteria.

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Section: Pili Of Gram-negative Bacteriamentioning

confidence: 99%

Pili in Gram-negative and Gram-positive bacteria — structure, assembly and their role in disease

Proft

Baker

2008

Cell. Mol. Life Sci.

449

406

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“…Indeed, NE-induced release of Dr fimbriae from Afa/Dr DAF/CEACAMs DAEC has been observed. This is due to the differential induction of the genes draC, which encodes the usher, and draE, which encodes the major fimbrial subunit (804). Like for Afa/Dr DAF/CEACAMs DAEC apically infecting intestinal cells, the released Dr fimbriae are able to induce hDAF-dependent phosphorylation of Erk1/2 MAPK and the production of IL-8 in fully differentiated Caco-2/TC7 cells (805).…”

Section: Host Cellular Defense Responsesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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“…For enteric pathogens including EPEC, ETEC, DAEC, and Y. enterocolitica, a temperature near 37°C is important for virulence (13,24,28,83). For EPEC, physiological osmolarity, near-neutral pH, and quorum sensing have all been implicated in pathogenesis (13,92).…”

mentioning

confidence: 99%

“…For EPEC, physiological osmolarity, near-neutral pH, and quorum sensing have all been implicated in pathogenesis (13,92). Toxin production and hence virulence in ETEC are regulated by osmolarity and microaerophilic conditions (83), while Dr fimbria production in DAEC is controlled by anaerobic conditions (24). Invasion by Y. enterocolitica is controlled by regulation of the expression of the invasin protein by an acidic pH (28).…”

mentioning

confidence: 99%

Perturbation of the Small Intestine Microbial Ecology by Streptomycin Alters Pathology in a Salmonella enterica Serovar Typhimurium Murine Model of Infection

Garner¹,

Antonopoulos²,

Wagner³

et al. 2009

Infect Immun

116

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The small intestine is an important site of infection for many enteric bacterial pathogens, and murine models, including the streptomycin-treated mouse model of infection, are frequently used to study these infections. The environment of the mouse small intestine and the microbiota with which enteric pathogens are likely to interact, however, have not been well described. Therefore, we compared the microbiota and the concentrations of short-chain fatty acids (SCFAs) present in the ileum and cecum of streptomycin-treated mice and untreated controls. We found that the microbiota in the ileum of untreated mice differed greatly from that of the cecum of the same mice, primarily among families of the phylum Firmicutes. Upon treatment with streptomycin, substantial changes in the microbial composition occurred, with a marked loss of population complexity. Characterization of the metabolic products of the microbiota, the SCFAs, showed that formate was present in the ileum but low or not detectable in the cecum while butyrate was present in the cecum but not the ileum. Treatment with streptomycin altered the SCFAs in the cecum, significantly decreasing the concentration of acetate, propionate, and butyrate. In this work, we also characterized the pathology of Salmonella infection in the ileum. Infection of streptomycin-treated mice with Salmonella was characterized by a significant increase in the relative and absolute levels of the pathogen and was associated with more severe ileal inflammation and pathology. Together these results provide a better understanding of the ileal environment in the mouse and the changes that occur upon streptomycin treatment.

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Environmental signals implicated in Dr fimbriae release by pathogenic Escherichia coli

Cited by 11 publications

References 30 publications

Pili in Gram-negative and Gram-positive bacteria — structure, assembly and their role in disease

Pili in Gram-negative and Gram-positive bacteria — structure, assembly and their role in disease

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Perturbation of the Small Intestine Microbial Ecology by Streptomycin Alters Pathology in a Salmonella enterica Serovar Typhimurium Murine Model of Infection

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