Environmental-friendly pretreatment and process optimization of macroalgal biomass for effective ethanol production as an alternative fuel using Saccharomyces cerevisiae

Ruangrit, Khomsan; Chaipoot, Supakit; Phongphisutthinant, Rewat; Kamopas, Wassana; Jeerapan, Itthipon; Pekkoh, Jeeraporn; Srinuanpan, Sirasit

doi:10.1016/j.bcab.2021.101919

Cited by 11 publications

(4 citation statements)

References 39 publications

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“…The exhaustion of fossil fuels and the limitation of natural gas require enormous effort to produce renewable energy from environmentally friendly and sustainable sources. Biomass fuel ethanol is a valid candidate for renewable and sustainable fuel manufacturing (Zabed et al 2016 ; Ruangrit et al 2021 ). The conversion of lignocellulosic feedstocks to fuel ethanol requires pretreatment, enzymatic hydrolysis, and fermentation (Deshavath et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic analysis of hydroxycinnamic acid inhibition on Saccharomyces cerevisiae

Ge,

Chen,

et al. 2024

Appl Microbiol Biotechnol

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Ferulic acid (FA) and p-coumaric acid (p-CA) are hydroxycinnamic acid inhibitors that are mainly produced during the pretreatment of lignocellulose. To date, the inhibitory mechanism of hydroxycinnamic acid compounds on Saccharomyces cerevisiae has not been fully elucidated. In this study, liquid chromatography-mass spectrometry (LC–MS) and scanning electron microscopy (SEM) were used to investigate the changes in S. cerevisiae cells treated with FA and p-CA. In this experiment, the control group was denoted as group CK, the FA-treated group was denoted as group F, and the p-CA-treated group was denoted as group P. One hundred different metabolites in group F and group CK and 92 different metabolites in group P and group CK were selected and introduced to metaboanalyst, respectively. A total of 38 metabolic pathways were enriched in S. cerevisiae under FA stress, and 27 metabolic pathways were enriched in S. cerevisiae under p-CA stress as identified through Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis. The differential metabolites involved included S-adenosine methionine, L-arginine, and cysteine, which were significantly downregulated, and acetyl-CoA, L-glutamic acid, and L-threonine, which were significantly upregulated. Analysis of differential metabolic pathways showed that the differentially expressed metabolites were mainly related to amino acid metabolism, nucleotide metabolism, fatty acid degradation, and the tricarboxylic acid cycle (TCA). Under the stress of FA and p-CA, the metabolism of some amino acids was blocked, which disturbed the redox balance in the cells and destroyed the synthesis of most proteins, which was the main reason for the inhibition of yeast cell growth. This study provided a strong scientific reference to improve the durability of S. cerevisiae against hydroxycinnamic acid inhibitors. Key points • Morphological changes of S. cerevisiae cells under inhibitors stress were observed. • Changes of the metabolites in S. cerevisiae cells were explored by metabolomics. • One of the inhibitory effects on yeast is due to changes in the metabolic network.

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Section: Introductionmentioning

confidence: 99%

Metabolomic analysis of hydroxycinnamic acid inhibition on Saccharomyces cerevisiae

Ge,

Chen,

et al. 2024

Appl Microbiol Biotechnol

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“…Global greenhouse gas emissions are already recorded at 52 GtCO 2 e (tons of carbon dioxide equivalent) and it is estimated that from 2030 onwards, emissions of these pollutants could reach 58 GtCO 2 e per year. However, it has been extensively discussed that to keep global warming below 1.5 • C, the emissions of these gases must be reduced up to 30 GtCO 2 e per year by 2030 [1]. Despite efforts to reach the desired levels on the world stage, the use of renewable energies and the inclusion of biofuels does not seem to exceed 20% of global consumption, even when the demand for biofuels, such as bioethanol, biodiesel, HVO, biogas and biobutanol, has increased each year.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, van Hal et al [18] consider that the energy costs of harvesting are not so troublesome for macroalgae due to their larger size, although they do not present a significant content of lipids compared to microalgae. Besides, macroalgae, also known as seaweeds, are attractively evaluated as a feedstock nowadays for the production of biofuels, including biomethane [19][20][21][22]; bio-oil [23][24][25]; bioethanol [26][27][28][29][30] and biodiesel [31][32][33][34][35][36][37][38][39], and to attain several important bioproducts for chemical and pharmaceutical applications [40].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production from Macroalgae Oil from Fucus vesiculosus Using Magnetic Catalyst in Unconventional Reactor Assisted by Magnetic Field

Silveira¹,

Souza²,

Pérez³

et al. 2022

Magnetochemistry

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A novel magnetic catalyst with hollow cylinder shape based on K2CO3/γ-Al2O3/Sepiolite/CoFe2O4 was prepared to convert macroalgae oil (Fucus vesiculosus) into biodiesel in an unconventional reactor assisted by magnetic field. Catalysts were formulated by the extrusion and characterized satisfactorily by physicochemical (mechanical strength, XRD, TG/DTG, FTIR and TPD-CO2), magnetic (VSM and EPR), morphological (SEM) and textural properties (BET). While their catalytic performance was also evaluated at 70 °C, oil: ethanol molar ratio 1:12 and 6 wt.% of catalyst using two different reaction systems for comparative purposes: (a) conventional stirred reactor and (b) fluidized bed reactor assisted by a magnetic field. The attained biodiesel presents properties in accordance with the standard limits (ASTM and EN) and total conversion (>99%) was observed in both cases after 2 h of reaction without significant differences between the two reactors. However, the magnetic properties of these catalysts allowed stabilization of the bed under a magnetic field and easy magnetic catalyst separation/recovery at the reaction end, showing their great potential for biodiesel production with regard to conventional process and thus, transforming it into a more sustainable technology.

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“…Crude oil and natural gas are traditionally the main raw materials for producing fuels and industrial chemicals. However, human dependency on fossil fuels has become a critical issue worldwide and it is essential to explore new sustainable and alternative sources to solve the world's environmental problems [5]. Ethanol is an important source of biofuels and the ethanol development and implementation is within the scope of circular bioeconomy since biofuels can substitute for fossil carbon by bio-based carbon in biomass from agriculture, forestry and municipal wastes.…”

Section: Introductionmentioning

confidence: 99%

Recent Developments and Current Status of Commercial Production of Fuel Ethanol

Hoang¹,

Nghiem

2021

Fermentation

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Ethanol produced from various biobased sources (bioethanol) has been gaining high attention lately due to its potential to cut down net emissions of carbon dioxide while reducing burgeoning world dependence on fossil fuels. Global ethanol production has increased more than six-fold from 18 billion liters at the turn of the century to 110 billion liters in 2019, only to fall to 98.6 billion liters in 2020 due to the pandemic. Sugar cane and corn have been used as the major feedstocks for ethanol production. Lignocellulosic biomass has recently been considered as another potential feedstock due to its non-food competing status and its availability in very large quantities. This paper reviews recent developments and current status of commercial production of ethanol across the world with a focus on the technological aspects. The review includes the ethanol production processes used for each type of feedstock, both currently practiced at commercial scale and still under developments, and current production trends in various regions and countries in the world.

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Environmental-friendly pretreatment and process optimization of macroalgal biomass for effective ethanol production as an alternative fuel using Saccharomyces cerevisiae

Cited by 11 publications

References 39 publications

Metabolomic analysis of hydroxycinnamic acid inhibition on Saccharomyces cerevisiae

Metabolomic analysis of hydroxycinnamic acid inhibition on Saccharomyces cerevisiae

Biodiesel Production from Macroalgae Oil from Fucus vesiculosus Using Magnetic Catalyst in Unconventional Reactor Assisted by Magnetic Field

Recent Developments and Current Status of Commercial Production of Fuel Ethanol

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