“…Biosynthesis of these two metabolites is coordinated by a complex regulatory network involving autoinduction and mutual repression (Schnider-Keel et al, 2000;Brodhagen et al, 2004;Baehler et al, 2005;Kidarsa et al, 2011) with carbon source composition being a major factor mediating their relative production (Nowak-Thompson et al, 1994;Kraus and Loper, 1995;Duffy and Défago, 1999). The carbon source for the culture experiments was glycerol, which is favourable for pyoluteorin production (Nowak-Thompson et al, 1994;Kraus and Loper, 1995;Duffy and Défago, 1999), while the main carbohydrates detected in pea seed exudates were sucrose, galactose, mannose and fructose (Table S1). While we cannot conclude that carbon source was responsible for the observed differences in secondary metabolite production or gene expression on seeds versus in culture, the data presented in this study indicate the value of assessing gene expression of a biological control agent grown on a plant surface.…”