Environmental exposure to lead: Analytical and epidemiological investigations using the European standardised method for blood delta-aminolevulinic acid dehydratase activity determination

Берлин, А. А.; Kh, Schaller; Grimes, H.; Langevin, Mélissa; Trotter, James

doi:10.1007/bf00405658

Cited by 12 publications

(8 citation statements)

References 5 publications

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“…Blood lead levels were evaluated by atomic absorption spectrophotometry 9–11 . ALAD activity in blood was assayed using the standardised European method 12 . Student's t‐test was used for statistical analysis of metric variables that met the criteria for normal distributions (standardised kurtosis coefficient test <2, standardised skewness coefficient test <2, and variance ratio less than the value corresponding to the degrees of freedom).…”

Section: Methodsmentioning

confidence: 99%

Effect of Subclinical Lead Intoxication on Laryngeal Cancer

Kandiloris

Goletsos

Nikolopoulos

et al. 1997

Int J Clinical Practice

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SUMMARY In this study we investigated the possible relationship of laryngeal cancer and subclinical lead intoxication, using the depression of aminolevulinic acid dehydratase (ALAD) activity in blood as indicator. Twenty‐six patients with laryngeal cancer and 53 normal controls met the criteria to enter the study. Blood ALAD activity values in the patients with laryngeal cancer ranged from 27.1 to 75.3 U/l with a mean of 50.79 U/l. The respective values in the control group ranged from 36.2 to 98 U/l with a mean of 59.76 U/l. There was a statistically significant difference between the two means (0.001 <p<0.01), whereas blood lead concentrations in all patients were within normal limits. These findings support the hypothesis that low level lead intoxication (subclinical blood lead levels), from cars, industries and products, may contribute to the risk of laryngeal cancer. Further investigation is needed to clarify the exact relationship between lead and cancer of the larynx.

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Section: Methodsmentioning

confidence: 99%

Effect of Subclinical Lead Intoxication on Laryngeal Cancer

Kandiloris

Goletsos

Nikolopoulos

et al. 1997

Int J Clinical Practice

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“…The activity of erythrocytic &aminolevulinic acid dehydratase (ALAD) was measured immediately after blood collection, according to the standardized European method (13).…”

Section: Methodsmentioning

confidence: 99%

Bioavailability of Biologically Detoxified Lead: Risks Arising from Consumption of Polluted Mussels

Regoli¹,

Orlando²

1994

Environmental Health Perspectives

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The possible risk for human health arising from consumption of lead-polluted shellfish was suggested by experiments on the bioavailability for mice of a "biologically detoxified" form of the metal. In this work, young mice were fed with a mixed diet including mussels collected in a Pb-polluted area. Metal concentrations in blood, kidney, liver, urine, and feces and the activity of erythrocytic delta-aminolevulinic acid dehydratase were determined after 1, 2, and 4 weeks. Comparisons were made with mice treated with balanced diet, mixed diet including control mussels, and drinking water with lead dissolved as acetate. In mice fed polluted mussels, lead concentrations increased in blood, kidney, and liver, whereas no significant accumulation was observed in urine. Different responses in mice treated with Pb(CH3COO)2 in drinking water are probably due to the diversity of lead chemical form in the two treatments. Our results demonstrate the bioavailability of biologically detoxified lead that can be transferred to a consumer with possible consequences also for human health.

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“…ALAD activity was measured within 6 h after sample collection using the European standardized method, with spectrophotometric (Unicam Helios UV-VIS spectrophotometer, Thermo Spectronic, Cambridge, UK) determination carried out at 555 nm [19,20]. The principle of the method of determining ALAD activity was based on incubation of the enzyme with excess δ-aminolevulinic acid.…”

Section: Alad Activitymentioning

confidence: 99%

Use of Generalized Additive Model to Detect the Threshold of δ-Aminolevulinic Acid Dehydratase Activity Reduced by Lead Exposure

Huang

Yang

Liu

et al. 2020

IJERPH

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Background: Lead inhibits the enzymes in heme biosynthesis, mainly reducing δ-aminolevulinic acid dehydratase (ALAD) activity, which could be an available biomarker. The aim of this study was to detect the threshold of δ-aminolevulinic acid dehydratase activity reduced by lead exposure. Methods: We collected data on 121 lead workers and 117 non-exposed workers when annual health examinations were performed. ALAD activity was determined by the standardized method of the European Community. ALAD G177C (rs1800435) genotyping was conducted using the polymerase chain reaction and restricted fragment length polymorphism (PCR-RFLP) method. In order to find a threshold effect, we used generalized additive models (GAMs) and scatter plots with smoothing curves, in addition to multiple regression methods. Results: There were 229 ALAD1-1 homozygotes and 9 ALAD1-2 heterozygotes identified, and no ALAD2-2 homozygotes. Lead workers had significantly lower ALAD activity than non-exposed workers (41.6 ± 22.1 vs. 63.3 ± 14.0 U/L, p < 0.001). The results of multiple regressions showed that the blood lead level (BLL) was an important factor inversely associated with ALAD activity. The possible threshold of BLL affecting ALAD activity was around 5 μg/dL. Conclusions: ALAD activity was inhibited by blood lead at a possible threshold of 5 μg/dL, which suggests that ALAD activity could be used as an indicator for lead exposure regulation.

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Environmental exposure to lead: Analytical and epidemiological investigations using the European standardised method for blood delta-aminolevulinic acid dehydratase activity determination

Cited by 12 publications

References 5 publications

Effect of Subclinical Lead Intoxication on Laryngeal Cancer

Effect of Subclinical Lead Intoxication on Laryngeal Cancer

Bioavailability of Biologically Detoxified Lead: Risks Arising from Consumption of Polluted Mussels

Use of Generalized Additive Model to Detect the Threshold of δ-Aminolevulinic Acid Dehydratase Activity Reduced by Lead Exposure

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