Environmental estrogens in an urban aquatic ecosystem: II. Biological effects

Schultz, Melissa M.; Minarik, Thomas; Martinović-Weigelt, Dalma; Curran, Erin M.; Bartell, Steve; Schoenfuss, Heiko L.

doi:10.1016/j.envint.2013.08.006

Cited by 47 publications

(41 citation statements)

References 72 publications

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“…It was observed that irrigation of Medicago sativa plants with sewage water, which contained 0.3 µg•L −1 estrogen, resulted in increased vegetative growth [17]. Although significant progress has been made in establishing the biological and ecological consequences of animal exposure to environmental estrogens (EEs) [6] [18], there is still a big gap of knowledge regarding the effects of EEs on plants, especially crop plants as sources of food for an exponentially growing human population.…”

Section: Introductionmentioning

confidence: 99%

“…Estrogens and estrogen-like compounds (xenoestrogens) from livestock manure, animal waste, and human waste (especially pharmaceutical waste), are being disposed of and excreted at high rates into the agricultural soil and ground water all over the world [1]- [6]. Thus, estrogen concentrations in the soil can range from 0.5 ng/L to 70 ng/L depending on the soil type and pollutant source [7].…”

Section: Introductionmentioning

confidence: 99%

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Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana

Upadhyay¹,

Maier²

2016

AJPS

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Mammalian sex hormones are spread in the environment from natural and anthropogenic sources. In the present study, the effect of estradiol on Arabidopsis thaliana growth primary metabolism, phenylpropanoid and flavonoid pathways and pathogen resistance were investigated. Treatments of Arabidopsis plants with 10 and 100 nM 17β-estradiol resulted in enhanced root growth and shoot biomass. In addition, treated plants had an increased rate of photosynthesis with a concomitant increase in carbohydrate and protein accumulation. Plants exposed to higher concentrations of 17β-estradiol (10 μM) had significantly lower root growth, biomass, photosynthesis rate, primary metabolite and phenylpropanoid and flavonoid contents indicating a toxic effect of estradiol. Treatments with increasing estradiol concentrations (10 nM, 100 nM and 10 μM) resulted in the downregulation of phenylpropanoid-flavonoid pathway genes (PAL1, PAL4, CHI and ANS) and subsequent decreased accumulation of phenolics, flavonoids and anthocyanins. Estradiol-treated plants were inoculated with Pseudomonas syringae pv. Tomato DC3000 and basal resistance was determined. Estradiol treatments rendered plants susceptible to the pathogen, thus compromising the plant defense mechanisms. These results indicate that at low concentrations, estradiol functions as a biostimulant of growth, yield and primary metabolism of Arabidopsis. However, estradiol functions as a potential transcriptional regulator of the phenylpropanoid pathway genes in Arabidopsis, having a negative effect on the phenylpropanoid and flavonoid biosynthetic pathways.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana

Upadhyay¹,

Maier²

2016

AJPS

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“…E1-L and E1-M exposure concentrations in the current study represent "worst case scenarios" for environmental E1 concentrations as found in some effluent dominated systems (Schultz et al, 2013) but are lower than reported total estrogenicity in other effluent dominated systems (Alvarez et al, 2013;Kolpin et al, 2002;Schultz et al, 2013). Fish populations in northern latitudes are also frequently drawn to effluent outfalls as temperature refugia during cold winter months when ambient water temperature may approach freezing.…”

Section: Discussionmentioning

confidence: 98%

Temperature modulates estrone degradation and biological effects of exposure in fathead minnows

Cox

Peterson

Tan

et al. 2018

Science of The Total Environment

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• Estrone and effluent temperature interact to affect fish with unknown consequences.• Identified effects of temperature on E1 degradation in wastewater and exposed fish.• Ran nitrification experiments and fish estrone exposures at multiple temperatures.• Cooler temperatures slowled E1 degradation and produced sex-specific effects in fish.• Seasonal variation modifies E1 degradation in wastewater and E1 exposure in fish. Environmental pollutants, including estrogens, are widespread in aquatic environments frequently as a result of treated wastewater effluent discharged. Exposure to estrogens has been correlated with disruption of the normal physiological and reproductive function in aquatic organisms, which could impair the sustainability of exposed populations. However, assessing the effects of estrogen exposure on individuals is complicated by the fact that rates of chemical uptake and environmental degradation are temperature dependent. Because annual temperature regimes often coincide with critical periods of biological activity, temperature-dependent changes in estrogen degradation efficacy during wastewater treatment could modulate biological effects. We examined the interactions between ambient water temperature and degradation of estrone (E1) during wastewater treatment. In addition, we exposed mature fathead minnows (Pimephales promelas) to three environmentally relevant concentrations of E1 at four different water temperatures (15°C, 18°C, 21°C, and 24°C) to reflect natural seasonal variation. E1 degradation occurred with and without the support of robust nitrification at all temperatures; however, the onset of E1 degradation was delayed at cooler water temperatures. In addition, we observed significant interactive effects between temperature and E1 exposure. Female morphometric endpoints were more susceptible to temperature-modulating effects while physiological endpoints were more strongly affected in males. Collectively, the data demonstrate that natural seasonal fluctuations in temperature are sufficient to affect E1 degradation during wastewater treatment and induce sex-dependent physiological and anatomical changes in exposed fish. G R A P H I C

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“…We chose the endangered Rio Grande silvery minnow and the fathead minnow, commonly used as a laboratory model species, because both species are found in the Middle Rio Grande. We also assessed exposure effects in bluegill sunfish (Lepomis macrochirus) because this species is sometimes used in field studies including some assessing the environmental effects of estrogenic EACs (Teather and Parrott 2006;Writer et al 2010;Schultz et al 2013). In addition, Rio Grande silvery minnows and fathead minnows are closely related species in the Cyprinidae family, whereas the bluegill sunfish is a phylogenetically more distant species in the family Centrarchidae.…”

mentioning

confidence: 99%

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

Jorgenson

Buhl

Bartell

et al. 2014

Arch Environ Contam Toxicol

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Although the effects of estrogens on model laboratory species are well documented, their utility as surrogates for other species, including those listed as endangered, are less clear. Traditionally, conservation policies are evaluated based on model organism responses but are intended to protect all species in an environment. We tested the hypothesis that the endangered Rio Grande silvery minnow (Hybognathus amarus) is more vulnerable to endocrine disruption-as assessed through its larval predator-escape performance, survival, juvenile sex ratios, and whole-body vitellogenin concentration-than the commonly used toxicological model species fathead minnow (Pimephales promelas) and the bluegill sunfish (Lepomis macrochirus). Fish were exposed concurrently for 21 days to the model endocrine active compound (EAC) 17ß-estradiol (E2) at 10 ng E2/L and 30 ng E2/L in a flow-through system using reconstituted water that simulated the physicochemical conditions of the Middle Rio Grande in New Mexico, USA. No significant differences were observed between the fathead and silvery minnow in larval predator-escape response or juvenile sex ratio. Rio Grande silvery minnow survival decreased significantly at day 14 compared with the other two species; by day 21, both cyprinid species (silvery minnow and fathead minnow) exhibited a significant decrease in survival compared with bluegill sunfish, a member of the family Centrarchidae. Male Rio Grande silvery minnow showed a significant increase in whole-body vitellogenin concentration in the 10 ng/L treatment, whereas fathead minnow and bluegill sunfish showed no significant increases in vitellogenin concentrations across treatments. Our study showed response differences to estrogen exposures between the two cyprinid species and further divergence in responses between the families Cyprinidae and Centrarchidae. These results suggest that commonly used laboratory model organisms may be less sensitive to EACs than the endangered Rio Grande silvery minnow. However, this study supports the continued use of surrogate species for the beneficial implementation of water-quality regulations for the protection of threatened and endangered species if phylogenetic relationships are taken into consideration.

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Environmental estrogens in an urban aquatic ecosystem: II. Biological effects

Cited by 47 publications

References 72 publications

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Temperature modulates estrone degradation and biological effects of exposure in fathead minnows

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

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Environmental estrogens in an urban aquatic ecosystem: II. Biological effects

Cited by 47 publications

References 72 publications

Effects of 17&lt;i&gt;β&lt;/i&gt;-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Effects of 17&lt;i&gt;β&lt;/i&gt;-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Temperature modulates estrone degradation and biological effects of exposure in fathead minnows

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

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Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>