Environmental estrogenic effects and gonadal development in wild goldfish (Carassius auratus)

Li, Chun-Ri; Lee, Sanghoon; Kim, So‐Sun; Kim, Andre; Lee, Keun Woo; Lü, Ming; Kim, Hee‐Eun; Kwak, Im-Jung; Lee, Yun-Ju; Kim, Dong‐Kyoo; Lee, Jung-Sick; Kang, Shin‐Won; Huh, Min-Do; Chung, Kyu-Hyuck; Park, Jang-Su

doi:10.1007/s10661-008-0238-1

Cited by 16 publications

(5 citation statements)

References 21 publications

(18 reference statements)

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“…The levels of HSP70 in the cultured hepatocytes were detected by western blot using the polyclonal antibody of goldfish and the monoclonal antibody of mouse. These results refer to the previous studies of this laboratory [13]. The HSP70 expression at 25°C was used as a control to normalize the expression of other temperatures.…”

Section: Resultssupporting

confidence: 85%

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Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Kim¹,

So²,

Park³

2016

Journal of Life Science

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Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at 33°C and showed slightly at 37°C, whereas mouse HSF1 (mHSF1) did so at 42°C. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from 25°C to 42°C. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.

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Section: Resultssupporting

confidence: 85%

“…In Korea, goldfish (Carassius auratus) are distributed widely in both polluted and unpolluted water. This species has been used as a model to evaluate environmental contamination [13]. This study was designed to show the trimerization of HSF1s (mHSF and gfHSF1) exposed to thermal stress.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Kim¹,

So²,

Park³

2016

Journal of Life Science

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“…But, even at these low levels, they were found to be biologically active (Hibberd et al 2009;Lu et al 2010b). Feminization of wild fish has been reported in many countries (Hashimoto et al 2000;Hu et al 2003;Vethaak et al 2005;Deng et al 2007;Li et al 2009;Randak et al 2009). Feminized males are most often observed near wastewater outfalls or in areas receiving large amount of domestic or industrial wastewater.…”

Section: Introductionmentioning

confidence: 99%

Assessment of estrogenic contamination and biological effects in Lake Taihu

Yan

Wang

et al. 2011

Ecotoxicology

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Lake Taihu is the third largest freshwater lake in China and is contaminated with xenoestrogens associated with high population density, intensive livestock and aquatic breeding activities. A field study in Lake Taihu was conducted using the goldfish (Carassius auratus) as an indicator organism. Several biological markers were selected to assess the extent of estrogenic contamination. Changes in serum vitellogenin (VTG), and gill 7-Ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST) and reduced glutathione (GSH) were measured in caged juvenile goldfish for 28 days in seven locations in northern Lake Taihu. Bioassay showed VTG increased 0.64-2.42 folds over time in goldfish collected from five stations and GSH decreased in samples from all seven stations after 7 days of exposure. EROD levels increased continually in fish collected at all the seven stations and the highest concentrations occurred at day 21. GST activity increased significantly at 7 days. The concentration of the target estrogens estrone (E(1)), 17β-estradiol (E(2)), ethinylestradiol (EE(2)), octylphenol (OP), diethylstilbestrol (DES), nonylphenol (NP) and bisphenol A (BPA) were determined in lake water at the sampling stations. Each individual estrogen concentration measured was multiplied by its relative potency to gain the estradiol equivalent (EEQ). There was an obvious correlation between the concentration of VTG and the total EEQ for all seven locations (P < 0.001). The biomarker VTG, EROD, GST and GSH assays and chemical analysis might be used to illustrate the potential risk in Lake Taihu.

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“…Steroid estrogens, known as typical endocrine disrupting chemicals (EDCs), are widely distributed in various aquatic environments, such as rivers, lakes, oceans, groundwater and drinking water. [1][2][3][4] Human and animal excretions are considered to be the main sources of these steroid estrogens. Estrogens release from humans eventually enter wastewater treatment plants (WWTPs) within municipal wastewater.…”

Section: Introductionmentioning

confidence: 99%

Enhanced adsorption behavior of 17β‐estradiol by anaerobic granular sludge combined with zero‐valent iron

Zhao

Chen

Zhai

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND The release of natural estrogen 17 β‐estradiol (E2) to the aquatic environment is a matter of concern. Batch experiments were conducted to study the adsorption of E2 by anaerobic granular sludge (AnGS) combined with zero‐valent iron (ZVI). The enhancement mechanism of ZVI for E2 adsorption by AnGS was also analyzed. RESULTS The E2 adsorption efficiency of AnGs with ZVI was 48% higher than that without ZVI. The optimal conditions for high E2 adsorption efficiency (92.21%) and high equilibrium adsorption capacity (4.92 µg g‐1 VSS) were rZVI/AnGS 1.33, pH 6, and temperature 25°C. E2 adsorption by ZVI/AnGS was a spontaneous and exothermic process, well described by the Freundlich isotherm. CONCLUSION The addition of a suitable amount of ZVI enhanced E2 adsorption by AnGS. © 2017 Society of Chemical Industry

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Environmental estrogenic effects and gonadal development in wild goldfish (Carassius auratus)

Cited by 16 publications

References 21 publications

Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Assessment of estrogenic contamination and biological effects in Lake Taihu

Enhanced adsorption behavior of 17β‐estradiol by anaerobic granular sludge combined with zero‐valent iron

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