Steep regime, nature of alkaline liquor, and kilning conditions were studied for their effects on sorghum malt -amylase development in four Nigerian sorghum cultivars. Malt -amylase activity was markedly (p < .001) influenced by all the four factors as well as their various interactions. Overall, malts from KSV 8 variety were the most -amylolytic followed in sequence by those from Local Red (LR), SK 5912, and Local white (LW) respectively. The presence or absence of air rests in steep regimes was a significant (p < .001) determinant of sorghum -amylase response to final warm steeping, steep liquor and kilning condition. The nature of the alkaline steep liquor was also a major determinant of the pattern of malt -amylase response to the kilning condition. Steeping in Ca(OH) 2 enhanced malt -amylase activity at the higher temperature of kilning, while KOH produced the opposite effect. Ca(OH) 2 enhancement of -amylase development, at a kilning temperature of 50°C, was varietydependent suggesting that different sorghum cultivars may employ different biosynthesis models for -amylase synthesis. The regime-dependence of -amylase response to kiln temperature suggests that this was an important modulator of sorghum germination physiology.Key words: Alkaline liquor, -amylase, kilning temperature, sorghum malt, steeping regime, cultivars.
-2863(9'8-32-Amylase is the key saccharifying enzyme of the brewers' malt 22 . While the low -amylase activity of sorghum malts has been defined as one of the most serious obstacles to its use as replacement for barley malt 2 , results of recent studies using some improved varieties of the grain suggest that the production of high -amylasecontaining sorghum malts may be possible with the proper choice of variety and conditions of malting 9,10,[25][26][27] . Recent research has shown that sorghum malt ␣-amylase level is critically correlated with environmental conditions of steeping and kilning 24 . While some work has been conducted on the effects of environmental conditions of steeping on sorghum malt -amylase levels, very few data are available with respect to the effects of kilning conditions on the development of the sorghum enzyme 29 . In this communication, we present the effects of kilning conditions on the -amylase activity of sorghum malts prepared from different cultivars, and under varying conditions of steeping including dissimilar steep liquors as well as different steeping regimes.
1%8)6-%07 %2( 1)8,3(7 7SYVGIW SJ WSVKLYQ GYPXMZEVWCultivars SK 5912 and KSV 8 were improved varieties obtained from the National Seeds Service, Zaria, Nigeria. Grains of the local white (LW) and the local red (LR) varieties were purchased from the open market at Nsukka, Nigeria. Germinative energies were above 95% for all the grains and none was water-sensitive.
7SVKLYQ QEPXMRKThe grains were sorted manually to remove broken kernels and debris. Alkaline steeping was carried out using glass-distilled water containing 0.1% of the alkali (NaOH, KOH, or Ca(OH) 2 ). Double glass-distilled...