Environmental Effects on Bee Microbiota

Nguyen, Phuong Nam; Rehan, Sandra M.

doi:10.1007/s00248-023-02226-6

Cited by 4 publications

(5 citation statements)

References 113 publications

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“…The diverse array of bacterial communities in Ontario included Acinetobacter and Enterobacter, but notably lacked Apilactobacillus (Figure 5A). This is consistent with patterns noted in the small carpenter bee Ceratina calcarata, where the normally ubiquitous Apilactobacillus was present in low relative abundances in Ontario (Nguyen & Rehan, 2022b), despite being previously characterised as a core bacterium and being found in almost all other bees (Graystock et al, 2017;Martinson et al, 2011;Nguyen & Rehan, 2022b, 2023aShell & Rehan, 2022). Martinson et al (2011) similarly found the absence of the bacterial phylotypes associated with the genus Lactobacillus.…”

Section: The Microbiome Across a Geographical Rangesupporting

confidence: 85%

“…Across all samples, we did not expect any potential impact of ethanol storage or washing on microbiome composition, as Hammer et al (2015) discovered that there were limited effects of surface sterilisation on microbiome characterisations and storage in 95% ethanol when compared to other storage methods. However, Apilactobacillus are found in practically all studied bees (Kwong & Moran, 2016; Martinson et al, 2011; Nguyen & Rehan, 2023a; Shell & Rehan, 2022), playing important roles in building immunity (Daisley et al, 2017; Daisley, Pitek, et al, 2020) and aiding in host learning and memory behaviour (Zhang et al, 2022). Although potentially correlated to patterns of low relative abundances of Apilactobacillus in urban Ontario (Nguyen & Rehan, 2022b) and in agreement with Martinson et al (2011), which found the absence of Firm‐4 and Firm‐5 bacterial phylotypes in A. virescens , considerations should be made regarding the effectiveness of certain bacterial characterisation depending on collection method.…”

Section: Discussionmentioning

confidence: 99%

“…The core microbiome of A. virescens across provinces consists of the bacteria Apilactobacillus and Acinetobacter, as well as the fungi Cladosporium, Alternaria and Aureobasidium (Table 1). These two bacteria are abundant and beneficial in other wild bees, although not previously detected in A. virescens (Martinson et al, 2011;Nguyen & Rehan, 2023a;Shell & Rehan, 2022). The three core fungal genera maintain close relationships with plants: Alternaria is a plant pathogen that is found in healthy honey bee larvae due to its antagonistic relationship with brood diseases (Ye et al, 2021), while Cladosporium and Aureobasidium support pollen preservation through degradation and fermentation, ultimately aiding in nutrient utilisation for honey bees (Disayathanoowat et al, 2020;Hsu et al, 2021;Parish et al, 2020;Rutkowski et al, 2023).…”

Section: Determining the Core Microbiomementioning

confidence: 97%

“…In comparison, solitary and facultatively social bees gain more of their microbiomes from their environment (Cohen et al, 2022;Dew et al, 2020;Graystock et al, 2017;Keller et al, 2021;McFrederick et al, 2012McFrederick et al, , 2017, bolstering more variable microbiota (Dew et al, 2020;McFrederick et al, 2017;Sookhan et al, 2021). Studies focusing on wild bee microbiomes are currently limited yet remain ongoing (Chau et al, 2023;Cohen et al, 2020;Handy et al, 2022;Kapheim et al, 2021;Nguyen & Rehan, 2023a;Shell & Rehan, 2022). Therefore, characterising the microbial ecology of additional bee species is necessary to compare differences in symbioses underpinning bee health.…”

Section: Introductionmentioning

confidence: 99%

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Microbiome and floral associations of a wild bee using biodiversity survey collections

Nguyen,

Samad‐zada,

Chau

et al. 2024

Environmental Microbiology

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The health of bees can be assessed through their microbiome, which serves as a biomarker indicating the presence of both beneficial and harmful microorganisms within a bee community. This study presents the characterisation of the bacterial, fungal, and plant composition on the cuticle of adult bicoloured sweat bees (Agapostemon virescens). These bees were collected using various methods such as pan traps, blue vane traps and sweep netting across the northern extent of their habitat range. Non‐destructive methods were employed to extract DNA from the whole pinned specimens of these wild bees. Metabarcoding of the 16S rRNA, ITS and rbcL regions was then performed. The study found that the method of collection influenced the detection of certain microbial and plant taxa. Among the collection methods, sweep net samples showed the lowest fungal alpha diversity. However, minor differences in bacterial or fungal beta diversity suggest that no single method is significantly superior to others. Therefore, a combination of techniques can cater to a broader spectrum of microbial detection. The study also revealed regional variations in bacterial, fungal and plant diversity. The core microbiome of A. virescens comprises two bacteria, three fungi and a plant association, all of which are commonly detected in other wild bees. These core microbes remained consistent across different collection methods and locations. Further extensive studies of wild bee microbiomes across various species and landscapes will help uncover crucial relationships between pollinator health and their environment.

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Section: The Microbiome Across a Geographical Rangesupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Determining the Core Microbiomementioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microbiome and floral associations of a wild bee using biodiversity survey collections

Nguyen,

Samad‐zada,

Chau

et al. 2024

Environmental Microbiology

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“…It appears that honey bees share a simple and constant core microbiome worldwide. This core microbiome can be identified as a baseline situation corresponding to a healthy microbiome in honey bees (Nguyen and Rehan, 2023).…”

Section: B1 the Microbiome In Pesticide Risk Assessment -Why Consider...mentioning

confidence: 99%

Roadmap for the integration of environmental microbiomes in risk assessments under EFSA's remit

Debode,

Caulier,

Demeter

et al. 2024

EFSA Supporting Publications

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Environmental microbiomes roadmap www.efsa.europa.eu/publications EFSA Supporting publication 2024:EN-8602The present document has been produced and adopted by the bodies identified above as author(s). In accordance with Article 36 of Regulation (EC) No 178/2002, this task has been carried out exclusively by the author(s) in the context of a grant agreement between the European Food Safety Authority and the author(s). The present document is published complying with the transparency principle to which the Authority is subject. It cannot be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors.

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Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Peters,

Leonhardt,

Schloter

et al. 2024

Preprint

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The global decline in biodiversity and insect populations highlights the urgent to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats to these essential services. Changes in land use alter resource and nest site availability, pesticide exposure and other factors impacting the richness, diversity, and health of solitary bee species. In this study, we investigated yet another facet currently less well investigated in such context: Microbial communities associated with wild bees play crucial roles in larval development, metabolism, immunity and overall bee health. However, the drivers and dynamics of healthy microbiome in solitary bees are still poorly understood, especially regarding the direct and indirect effects of land use on the diversity and composition of these microbial communities. We examined bacterial communities in the offspring and nest materials of the Megachilid trap-nesting solitary bee, O. bicornis,along a gradient of land use intensification by 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, and food resources are known to influence microbial compositions in solitary bee species, we hypothesized that land use changes would alter resources available for food and nest material collection and thereby affecting the microbiomes in offspring and their nest environments. We anticipated reduced microbial diversity and altered composition with increased land use intensification, which is known to decrease the number and diversity of resources, including the pool of floral and soil bacteria in the surrounding environment. As expected, we observed significant shifts in the bacterial composition and diversity of bees and their nests across varying degrees of land use intensity, differing in management types and the availability of flowers. The Shannon diversity of bacteria in nest materials (larval pollen provision, soil nest enclosure) and larval guts decreased with increasing land use intensity. However, the pupae microbiome remained unaffected, indicating a reorganization of the microbiome during metamorphosis, which is not significantly influenced by land use and available resources. Our findings provide new insights into the factors shaping environmental transmission and changes in solitary bee microbiomes. This understanding is crucial for comprehending the impacts of intensive land use on wild bee health and developing strategies to mitigate these effects.

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Environmental Effects on Bee Microbiota

Cited by 4 publications

References 113 publications

Microbiome and floral associations of a wild bee using biodiversity survey collections

Microbiome and floral associations of a wild bee using biodiversity survey collections

Roadmap for the integration of environmental microbiomes in risk assessments under EFSA's remit

Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

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