Environmental Effects of Temperature and Water Potential on Mycelial Growth of Neocosmospora solani and Fusarium spp. Causing Dry Root Rot of Citrus

Ezrari, Said; Radouane, Nabil; Tahiri, Abdessalem; Amiri, Saïd; Lazraq, Abderrahim; Lahlali, Rachid

doi:10.1007/s00284-021-02570-1

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…Similar symptoms are commonly observed in vascular wilt, stem and root rot diseases caused by the genus Fusarium (Azil et al, 2021; Crespo et al, 2019; Ezrari et al, 2021; Guarnaccia et al, 2021; Pérez‐Hernández et al, 2020). Fusarium is a species‐rich genus of mycotoxigenic plant pathogens, and its taxonomy has long been unclear (Crous et al, 2021; Geiser et al, 2021; Munkvold et al, 2021; O'Donnell et al, 2020; O'Donnell, Whitaker, et al, 2022).…”

Section: Introductionsupporting

confidence: 59%

“…Approximately one third of all fusarial plant diseases have been attributed to species in the FSSC (Geiser et al, 2013;Sandoval-Denis & Crous, 2018). F. solani is also generally recognized as a soil-inhabiting, plant-pathogenic fungus that often causes root rot, stem canker and vascular wilt diseases (Crespo et al, 2019;Ezrari et al, 2021;Guarnaccia et al, 2021;Pérez-Hernández et al, 2020).…”

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confidence: 99%

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Characterization, phylogeny and pathogenicity of Fusarium solani causing quinoa basal stem rot in Shanxi Province, China

Yin,

Tian,

Peng

et al. 2023

Plant Pathology

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A new emerging disease of quinoa, quinoa basal stem rot (QBSR), has been recorded in Shanxi Province, China. Observed during the inflorescence emergence stage, the pathogen causes wilting, foliar chlorosis, leaf abscission and eventual death of quinoa. In this study, isolates were identified as Fusarium solani using morphology, molecular and phylogenetic analysis (using sequences of calmodulin, rDNA internal transcribed spacer region, RNA polymerase II B‐subunits RPB1 RPB1 and RPB2 RPB2, and translation elongation factor 1‐α), and pathogenicity analysis. Twenty‐seven isolates were classified into two different groups according to their morphological characteristics, and the mycelial growth rate of Group 2 was higher than that of Group 1 on six different media. The optimum growth temperature for both groups was 30°C and the highest mycelial growth rates were 9.2 and 10.0 mm/day, for Groups 1 and 2, respectively. The sporodochial conidia (SC) and aerial conidia (AC) germinated normally at 15–25°C and 10–40°C, respectively, and the width of SC and AC germ tubes were 1.4 and 2.2 μm. The lethal temperature for AC was 51°C for 20 min, and SC stopped germinating at 45°C. Pathogenicity tests of representative isolates showed that mycelia or conidia caused typical symptoms of QBSR on wounded basal stems of Chenopodium quinoa at 6 days postinoculation. The optimum temperature for the onset of QBSR was 30°C for both groups of isolates, and lesion lengths ranged from 5.4 to 5.9 cm. Knowledge of this pathogen will provide useful information for effective disease management to prevent its spread.

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Section: Introductionsupporting

confidence: 59%

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confidence: 99%

Characterization, phylogeny and pathogenicity of Fusarium solani causing quinoa basal stem rot in Shanxi Province, China

Yin,

Tian,

Peng

et al. 2023

Plant Pathology

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“…It is possible that the F. oxysporum and F. solani strains were used after more than three series of cultures, which reduced their vitality. In addition, the optimal mycelium growth temperature of F. oxysporum and F. solani is 24-25℃ (Ezrari et al, 2021;Hibar et al, 2006;Jeon et al, 2013), and it is believed that mycelium growth was relatively inactive at 15-20℃, which is the condition used in this experiment. Among Fusarium-based pathogens, F. foetens and F. pseudoanthophilum are known to exhibit high molecular statistical exibility through their formation of a single system with F. oxysporum.…”

Section: Pathogenicity Test Of Dried Potato Strainmentioning

confidence: 99%

Identification and pathogenicity of species isolated from stored potato tubers showing symptoms of dry rot disease

Kim,

Hong,

Won

et al. 2023

Preprint

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Arises during the storage of potatoes as a result of the pathogens introduced during their growing and harvest. In this study, the causative bacteria of domestic potato dry rot disease were identified and their pathogenicity was confirmed. A total of 76 species were isolated from 93 potato necropods collected from samples inoculate with dry rot disease (Table 1). These 76 isolates were identified as Fusarium boothii, F. circinatum, F. citricola, F. foetens, F. iranicum, F. longifundum, F. oxysporum, F. pseudoanthophilum F. solani, Botryotinia ranunculi, Clonostachys rosea, and Humicola nigrescens. The average size of the inoculation site was ≥ 4.6 mm in F. oxysporum and F. solani, which were pathogenic to dry rot in potatoes but not as other strains for up to five weeks. The pathogenicity of F. foetens and F. pseudoanthophilum was related to high a molecular statistical flexibility by forming a single system with F. oxysporum. However, except for F. oxysporum and F. solani, these strains have not yet been reported to be associated with dry rot disease. Additionally, the length of the cross-section and longitudinal section of the potato sclera inoculated with C. rosea increased the most among all strains. This suggests that C. rosea is the dominant species involved in domestic potato dry rot disease. By contrast, there are no reports of the involvement of B. ranunculi and H. nigrescens in dry rot disease. Therefore, these strains can be seen as parasitic using potato sclerosis as nutrients in in vivo experiments through wounds and are not directly related to dry rot disease.

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“…Citrus is one of the most significant fruit crops grown nowadays in Morocco. The national citrus production area comprises 126.600 ha, and 2.6 million tons of various citrus fruits are produced each year [ 1 ]. However, citrus stubborn disease (CSD) continues to pose a severe danger to the citrus fruit industry and damage citrus production [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Serological and Molecular Methods for Detection of Spiroplasma citri in Moroccan Citrus-Growing Areas

Sagouti

Rhallabi

Polizzi

et al. 2023

Plants

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Spiroplasma citri, a helical motile, wall-less, and cultivable microorganism of the class Mollicutes, is the agent of the citrus stubborn disease. There is currently a lack of data about the presence of this pathogen in Moroccan citrus orchards. This study aims to validate serological and molecular methods for routine S. citri diagnosis in Moroccan citrus groves. To provide an update on the present status of the outbreak of the pathogen in Moroccan citrus orchards, a survey of S. citri was conducted in the main citrus-growing regions of Morocco. A total of 575 leaf samples were collected from citrus trees with symptoms attributable to S. citri infection. Samples were collected during 2020 and 2021 from 23 citrus orchards. The presence of S. citri was tested in all samples using the double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Using this method, 57 samples were found to be infected with S. citri, 41 samples had doubtful results, and the remaining samples were negative. To corroborate the results of the DAS-ELISA test, 148 samples were chosen for additional molecular testing using conventional polymerase chain reaction (PCR) and real-time PCR (qPCR) based on specific primer pairs targeting three different genes (putative adhesion-like gene P58, putative adhesion gene P89, and spiralin gene). Using primers that target the putative adhesion-like gene P58, S. citri was detected by conventional and real-time PCR amplification from plant tissue with differing degrees of specificity. The results allowed us to determine the incidence of S. citri in all Moroccan citrus orchards, with a wide range of positive samples varying from 6.5% to 78%, and to show that molecular tests, particularly real-time PCR assays that target the putative adhesion-like gene P58, are the most sensitive for making an accurate diagnosis of S. citri. Indeed, the real-time PCR with P58-targeting primers yielded positive results from all positive and doubtful ELISA samples as well as some negative samples, with an OD value close to 1.5 × times healthy samples, thus demonstrating a high sensibility of this technique.

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Environmental Effects of Temperature and Water Potential on Mycelial Growth of Neocosmospora solani and Fusarium spp. Causing Dry Root Rot of Citrus

Cited by 8 publications

References 39 publications

Characterization, phylogeny and pathogenicity of Fusarium solani causing quinoa basal stem rot in Shanxi Province, China

Characterization, phylogeny and pathogenicity of Fusarium solani causing quinoa basal stem rot in Shanxi Province, China

Identification and pathogenicity of species isolated from stored potato tubers showing symptoms of dry rot disease

Comparison of Serological and Molecular Methods for Detection of Spiroplasma citri in Moroccan Citrus-Growing Areas

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