Environmental DNA metabarcoding of intertidal meiofauna sheds light on its potential for habitat discovery

Wang, Meng; Yergaliyev, Timur; SUN, CHANG-HAI; Martinez, Joey Genevieve; Wang, Beixin

doi:10.1016/j.ecolind.2023.110223

Cited by 3 publications

(2 citation statements)

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“…In the last 15 years, DNA-and RNA-based surveys revolutionized research on marine plankton and benthos [40][41][42][43][44][45][46][47]. DNA metabarcoding was also utilized to characterize microbial eukaryotes in some intertidal habitats [48][49][50][51] and salt marsh soils [52], but not in tidal pools.…”

Section: Introductionmentioning

confidence: 99%

Microbial Eukaryotes in Natural and Artificial Salt Marsh Pools

Potapova,

Markarian,

King

et al. 2024

Coasts

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Microscopic eukaryotes are important components of coastal wetland ecosystems. The goal of this study was to investigate the diversity of microeukaryotes in the tidal pools of a New Jersey salt marsh and to compare the assemblages of natural and artificial pools excavated for controlling mosquito populations. We evaluated microeukaryotic assemblages using the amplicon sequencing of 18S and rbcL DNA markers and the microscopic identification of diatoms in water and sediment samples. 18S unique amplicon sequence variants (ASV) representing ciliates, dinoflagellates, diatoms, and cercozoans were the most diverse, while the reads of dinoflagellates, diatoms, ciliates, and nematodes were the most abundant. The dominant ASVs were attributed to organisms that are characteristic of coastal plankton and sediments or those known for their resistance to salinity, desiccation, hypoxia, and UV stress. The sediment assemblages were more diverse compared to those from the water column and contained a larger portion of ASVs that were not assigned to any low-rank taxa, reflecting the current gaps in understanding the diversity of microeukaryotes. Most taxonomic groups were significantly different in their abundance and composition between natural and artificial pools. Dinoflagellates, haptophytes, chrysophytes, pelagophytes, and raphidophytes—the groups that include a large proportion of mixotrophic taxa and species known for forming harmful algal blooms—were more abundant in the artificial than in the natural pools. Fungi, labyrinthulomycetes, and peronosporomycetes were also more abundant in artificial pools, which may be related to organic matter enrichment. Diatoms and foraminifera showed an opposite trend of higher abundance in natural pools.

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Section: Introductionmentioning

confidence: 99%

Microbial Eukaryotes in Natural and Artificial Salt Marsh Pools

Potapova,

Markarian,

King

et al. 2024

Coasts

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“…The increasing number of meiofaunal metabarcoding studies was listed in Gielings et al (2021) and Harbuzov et al (2022). A more recent study is Wang et al (2023). Danovaro et al (2008) investigated the Mediterranean deepsea nematode communities across a west-east transect and several deep-sea and shelf meiofaunal communities were studied in the Eastern Mediterranean (Danovaro et al, 2000;George, 2022;Lampadariou et al, 2009Lampadariou et al, , 2013Lampadariou & Eleftheriou, 2018;Lampadariou & Tselepides, 2006;Sevastou et al, 2013Sevastou et al, , 2020.…”

Section: Introductionmentioning

confidence: 99%

Deep‐sea meiofaunal communities in the south‐eastern Levantine basin and their shaping factors – Morphological‐taxonomy‐free metabarcoding approach

Harbuzov,

Farberova,

Tom

et al. 2024

Ecology and Evolution

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The <3% dissimilar Amplicon Sequence Variant (ASV) clusters of the 18S‐V4 barcode were used as species‐proxies for the evaluation of ASV composition and ASV diversity indices characterizing the hitherto poorly investigated meiofaunal communities of the south‐eastern part of the Levantine basin. Accompanied by abundance measurements, the relationships of these characteristics with sedimentary and bottom terrain parameters were interpreted. The construction of community composition profiles, namely ASVs' list and their estimated abundances, was done using our previously established procedure (Harbuzov et al., 2022, Marine Genomics 65, 100980), combining metabarcoding with sample reads normalization by the abundance of hard‐bodied meiofaunal taxa. The study province included the 54–1418 m depth range, across vertical sub‐bottom horizons ranging 0–17 cm. Oxygen, hydrogen sulfide and methane concentrations in the pore water, as well as sediment grain size spectra and sedimentary protein and carbohydrate levels, were measured, followed by an evaluation of their involvement in the shaping of the meiofaunal communities' characteristics. Community composition was generally site‐and‐horizon dependent and its abundance decreased with increasing bottom depth and across sub‐bottom horizons, typical to benthic habitats which are nourished by organic carbon from the euphotic zone. The relatively sharply inclined continental slope bottom located in the northern part of the Israeli coast was an exception. Its meiofaunal community characteristics were speculated to be affected by intensive sediment mixing and lateral transport of food from the shelf, in addition to the effect of the euphotic zone‐originated food sources.

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