Environmental DNA detection of the invasive mussel Mytella strigata as a surveillance tool

Yip, Zhi Ting; Lim, C.Y.H.; Tay, Y. C.; Tan, Yi; Beng, Stephen; Tun, Karenne; Teo, Serena Lay‐Ming; Huang, Danwei

doi:10.3391/mbi.2021.12.3.05

Cited by 5 publications

(1 citation statement)

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“…In addition, dedicated studies are still needed to understand how environmental factors (e.g., temperature, currents, tides, depth, stratification, and salinity) affect eDNA distribution and persistence dynamics in the marine environment, to optimize/support both sampling and results interpretation (Collins et al, 2018;Suarez-Bregua et al, 2022). In marine ecosystems, most studies have been conducted in coastal areas or estuaries (Crane et al, 2021;Ellis et al, 2022;Miralles et al, 2019Miralles et al, , 2016Yip et al, 2021), coastal lagoons (Ardura et al, 2017Muñoz-Colmenero et al, 2018), aquacultures (Brand et al, 2022Matejusova et al, 2021) and harbours and recreational marinas (Kim et al, 2018;Matejusova et al, 2021;Wood et al, 2017), whereas fewer have been conducted in the open sea (Catanese et al, 2022;Gargan et al, 2022;Wada et al, 2020) (Table S2).…”

Section: Edna-based Targeted Detection Of Rare Animal Species Has Bee...mentioning

confidence: 99%

Catch me if you can: current status and topical issues on the use of eDNA-based targeted detection of rare and endangered animal species

Duarte

Simões

Costa

2023

Preprint

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Animal detection through DNA present in environmental samples (eDNA) is a valuable tool for detecting rare species, that are difficult to observe and monitor. eDNA-based tools are underpinned by molecular evolutionary principles, which are key to devising tools to efficiently single out a targeted species from an environmental sample, using carefully chosen marker regions and customized primers. Here, we present a comprehensive review of the use of eDNA-based methods for the detection of targeted animal species, such as rare, endangered, or invasive species, through the analysis of 460 publications (2008-2022). Aquatic ecosystems have been the most surveyed, in particular, freshwaters (75%), and to a less extent marine (14%) and terrestrial systems (10%). Vertebrates, in particular, fish (38%), and endangered species, have been the most focused in these studies, and Cytb and COI are the most employed markers. Among invertebrates, assays have been mainly designed for Mollusca and Crustacea species (22%), in particular, to target invasive species, and COI has been the most employed marker. Targeted molecular approaches, in particular qPCR, have been the most adopted (73%), while eDNA metabarcoding has been rarely used to target single or few species (approx. 5%). However, less attention has been given in these studies to the effects of environmental factors on the amount of shed DNA, the differential amount of shed DNA among species, or the sensitivity of the markers developed, which may impact the design of the assays, particularly to warrant the required detection level and avoid false negatives and positives. The accuracy of the assays will also depend on the availability of genetic data from closely related species to assess both marker and primers specificity. In addition, eDNA-based assays developed for a particular species may have to be refined taking into account site-specific populations, as well as any intraspecific variation.

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Section: Edna-based Targeted Detection Of Rare Animal Species Has Bee...mentioning

confidence: 99%

Catch me if you can: current status and topical issues on the use of eDNA-based targeted detection of rare and endangered animal species

Duarte

Simões

Costa

2023

Preprint

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Complementary broad-range mitochondrial markers improve eDNA characterization of marine metazoan diversity

Yip,

Quek,

Huang

2023

Mar. Biodivers.

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Environmental performance indicators for the urban coastal environment of Singapore

Chng

Chou

Huang

2022

Regional Studies in Marine Science

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Environmental DNA detection of the invasive mussel Mytella strigata as a surveillance tool

Cited by 5 publications

References 0 publications

Catch me if you can: current status and topical issues on the use of eDNA-based targeted detection of rare and endangered animal species

Catch me if you can: current status and topical issues on the use of eDNA-based targeted detection of rare and endangered animal species

Complementary broad-range mitochondrial markers improve eDNA characterization of marine metazoan diversity

Environmental performance indicators for the urban coastal environment of Singapore

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