Environmental control of lignin peroxidase, manganese peroxidase, and laccase activities in forest floor layers in humid Asia

Fujii, Kazumichi; Uemura, Mari; Hayakawa, Chie; Funakawa, Shinya; Kosaki, Takashi

doi:10.1016/j.soilbio.2012.07.007

Cited by 79 publications

(52 citation statements)

References 45 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentrations of lignin (measured by the Klason method) in leaf litters varies depending on tropical tree species [e.g., 11% (Bignoniaceae) to 45% (Dipterocarpaceae) from Ostertag et al (2008) and Fujii et al (2012)]. Lignin and tannin typically retard decomposition and N release in temperate forests, as lignin is generally more recalcitrant than cellulose due to complicated structure (Talbot and Treseder 2012).…”

Section: Distribution Of Soil Animals and Microorganisms In Tropicalmentioning

confidence: 99%

Plant–soil interactions maintain biodiversity and functions of tropical forest ecosystems

Fujii

Shibata

Kitajima

et al. 2017

Ecological Research

Self Cite

107

View full text Add to dashboard Cite

Tropical forests are characterized by high biodiversity and aboveground biomass growing on strongly weathered soils. However, the distribution of plant species and soils are highly variable even within a tropical region. This paper reviews existing and novel knowledge on soil genesis, plant and microbial physiology, and biogeochemistry. Typically, forests in Southeast Asia are dominated by dipterocarps growing on acidic Ultisols from relatively young parent material. In the Neotropics and Africa, forests contain abundant legume trees growing on Oxisols developed in the older parent materials on stable continental shields. In Southeast Asia, the removal of base cations from the surface soil due to leaching and uptake by dipterocarp trees result in intensive acidification and accumulation of exchangeable Al 3+ , which is toxic to most plants. Nutrient mining by ectomycorrhizal fungi and efficient allocation within tree organs can supply phosphorus (P) for reproduction (e.g., mast fruiting) even on P-limited soils. In the Neotropics and Africa, nitrogen (N) fixation by legume trees can ameliorate N or P limitation but excess N can promote acidification through nitrification. Biological weathering [e.g., plant silicon (Si) cycling] and leaching can lead to loss of Si from soil. The resulting accumulation of Al and Fe oxides in Oxisols that can reduce P solubility through sorption and lead to limitation of P relative to N. Thus, geographical variation in geology and plant species drives patterns of soil weathering and niche differentiation at the global scale in tropical forests.

show abstract

Section: Distribution Of Soil Animals and Microorganisms In Tropicalmentioning

confidence: 99%

Plant–soil interactions maintain biodiversity and functions of tropical forest ecosystems

Fujii

Shibata

Kitajima

et al. 2017

Ecological Research

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…Based on the degree of decomposition, the litter horizon can typically be divided into a fresh litter (L) layer, a fermentation (F) layer and a humus (H) layer 1–3 . These three layers largely represent different degrees of decomposition of organic matter.…”

Section: Introductionmentioning

confidence: 99%

“…Below this layer is a layer that typically consists of a mixture of organic matter in different stages of decomposition, known as the F layer. The third layer is the H layer, which largely consists of humified material with little or no visible plant structure 1,2 . However, in some cases, the F layer is thin or absent and difficult to distinguish from the L layer.…”

Section: Introductionmentioning

confidence: 99%

Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau

Zheng

Chen

Liu

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Cellulose and lignin are the main polymeric components of the forest litter horizon. We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau. The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow). Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types. Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition. These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.

show abstract

“…Fujii et al showed that the humus (FH) layer is more acidic than the overlying litter (L) layer (34). In addition, Mn concentration in the FH layer was lower than that in the L layer.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Mn concentration in the FH layer was lower than that in the L layer. It was suggested that the HF layer is optimal (i.e., low pH and low Mn level) for LiP activity (34). Another example is the case of brown rot fungi that excrete oxalate to considerably lower the pH, especially in the immediate vicinity of fungal hyphae.…”

Section: Discussionmentioning

confidence: 99%

Limits of Versatility of Versatile Peroxidase

Knop

Levinson

Makovitzki

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Although Mn2؉ is the most abundant substrate of versatile peroxidases (VPs), repression of Pleurotus ostreatus vp1 expression occurred in Mn 2؉ -sufficient medium. This seems to be a biological contradiction. The aim of this study was to explore the mechanism of direct oxidation by VP1 under Mn 2؉ -deficient conditions, as it was found to be the predominant enzyme during fungal growth in the presence of synthetic and natural substrates. The native VP1 was purified and characterized using three substrates, Mn 2؉ , Orange II (OII), and Reactive Black 5 (RB5), each oxidized by a different active site in the enzyme. While the pH optimum for Mn 2؉ oxidation is 5, the optimum pH for direct oxidation of both dyes was found to be 3. Indeed, effective in vivo decolorization occurred in media without addition of Mn 2؉ only under acidic conditions. We have determined that Mn 2؉ inhibits in vitro the direct oxidation of both OII and RB5 while RB5 stabilizes both Mn 2؉ and OII oxidation. Furthermore, OII was found to inhibit the oxidation of both Mn 2؉ and RB5. In addition, we could demonstrate that VP1 can cleave OII in two different modes. Under Mn 2؉ -mediated oxidation conditions, VP1 was able to cleave the azo bond only in asymmetric mode, while under the optimum conditions for direct oxidation (absence of Mn 2؉ at pH 3) both symmetric and asymmetric cleavages occurred. We concluded that the oxidation mechanism of aromatic compounds by VP1 is controlled by Mn 2؉ and pH levels both in the growth medium and in the reaction mixture. IMPORTANCEVP1 is a member of the ligninolytic heme peroxidase gene family of the white rot fungus Pleurotus ostreatus and plays a fundamental role in biodegradation. This enzyme exhibits a versatile nature, as it can oxidize different substrates under altered environmental conditions. VPs are highly interesting enzymes due to the fact that they contain unique active sites that are responsible for direct oxidation of various aromatic compounds, including lignin, in addition to the well-known Mn 2؉ binding active site. This study demonstrates the limits of versatility of P. ostreatus VP1, which harbors multiple active sites, exhibiting a broad range of enzymatic activities, but they perform differently under distinct conditions. The versatility of P. ostreatus and its enzymes is an advantageous factor in the fungal ability to adapt to changing environments. This trait expands the possibilities for the potential utilization of P. ostreatus and other white rot fungi.

show abstract

Environmental control of lignin peroxidase, manganese peroxidase, and laccase activities in forest floor layers in humid Asia

Cited by 79 publications

References 45 publications

Plant–soil interactions maintain biodiversity and functions of tropical forest ecosystems

Plant–soil interactions maintain biodiversity and functions of tropical forest ecosystems

Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau

Limits of Versatility of Versatile Peroxidase

Contact Info

Product

Resources

About