Environmental Control and Photoautotrophic Micropropagation

Jeong, Byoung Ryong; Fujiwara, Kazuhiro; Kozai, Toyoki

doi:10.1002/9780470650585.ch4

Cited by 19 publications

(9 citation statements)

References 164 publications

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“…These plantlets usually need elevated CO 2 concentration and higher irradiance than conventionally used. 25,[50][51][52]65,66 Our results are in conformity with the above statement. In all the plant systems maximum F v / F m and ΦPS2 were obtained at either 10.0 or 40.0 gm -3 of CO 2 both in SFSM and SFLM.…”

Section: Discussionsupporting

confidence: 91%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

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Section: Discussionsupporting

confidence: 91%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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“…Micropropagation is one of the tissue culture technologies for rapidly multiplying genetically superior, physiologically uniform, and developmentally normal plantlets that cannot be propagated by seeds or whose vegetative propagation efficiency is low in a limited time (Jeong et al 1995). However, the widespread use of micropropagation is still limited due to high production costs (Aitken-Christie et al 1995).…”

Section: Introductionmentioning

confidence: 98%

Development and application of photoautotrophic micropropagation plant system

Xiao

Niu²,

Kozai

2010

Plant Cell Tiss Organ Cult

Self Cite

176

115

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Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic ability, but to the low CO 2 concentration in the air-tight culture vessel during the photoperiod. Moreover, numerous studies have been conducted on improving the in vitro environment and investigating its effects on growth and development of cultures/plantlets on nearly 50 species since the concept of photoautotrophic micropropagation was developed more than two decades ago. These studies indicate that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO 2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. This paper reviews the development and characteristics of photoautotrophic micropropagation systems and the effects of environmental conditions on the growth and development of the plantlets. The commercial applications and the perspective of photoautotrophic micropropagation systems are discussed.

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“…Indeed, to minimize transplantation constraints, the process of acclimatization may be initiated while the plantlets are still in vitro (Hazarika 2006). In photoautotrophic micropropagation systems Jeong et al 1995), plants develop in a culture medium without sugar, so growth or accumulation of carbohydrates is fully dependent upon photosynthesis and inorganic nutrient uptake (Kozai 1991;Zobayed et al 2004). Elmeskaoui et al (1995) and Cassells et al (1996) were the first to develop tripartite (strawberry plant+AMF associated with carrot root) and bipartite (strawberry+AMF from isolated spores) in vitro culture systems, respectively, for the colonization of micropropagated plantlets.…”

Section: Introductionmentioning

confidence: 99%

In vitro mycorrhization of banana (Musa acuminata) plantlets improves their growth during acclimatization

Koffi

Declerck

2015

In Vitro Cell.Dev.Biol.-Plant

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Micropropagated banana plantlets represent a good planting material to establish new growing areas or replace old plantations. The plantlets are devoid of pathogens but also of beneficial root inhabitants (e.g., endophytes and arbuscular mycorrhizal fungi [AMF]) that may help plants withstand stresses. Mycorrhization of banana is usually conducted at the hardening/post-hardening phases. Only a few studies have reported their application in vitro, and none have investigated the subsequent impact on ex vitro acclimatization. Here, we report for the first time the effects of in vitro mycorrhization of banana plantlets on growth following transfer to soil. Banana plantlets were pre-mycorrhized under autotrophic conditions. During in vitro culture, heavy root colonization of the banana plantlets was observed. At 5 and 7 wk after transfer to a peatsand soil substrate, the root system remained highly colonized. The growth (pseudostem height) and biomass were significantly higher than those of control plants grown on media without AMF. The mean shoot dry weight of the premycorrhized plants at 5 wk after transplanting was 66.7 and 34.6% greater than those of non-mycorrhized plantlets grown on modified Strullu-Romand (MSR) and Murashige and Skoog (MS) media, respectively, and was also greater at week 7 (by 64.7 and 40%, respectively). In vitro mycorrhization under autotrophic culture conditions thus represents a promising tool for the pre-adaptation of micropropagated banana plantlets to ex vitro conditions.

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Environmental Control and Photoautotrophic Micropropagation

Cited by 19 publications

References 164 publications

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Development and application of photoautotrophic micropropagation plant system

In vitro mycorrhization of banana (Musa acuminata) plantlets improves their growth during acclimatization

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