Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate animal model

Tiwari, Anjana; Pragya, Prakash; Ram, K. Ravi; Chowdhuri, D. Kar

doi:10.1016/j.theriogenology.2010.12.027

Cited by 42 publications

(20 citation statements)

References 220 publications

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“…Hence, much attention was paid on the toxicity study of pharmaceutical impurities and TPs in the environment [1][2][3]. However, traditional methods for toxicity or bioactivity studies, such as animal and cell experiments [4][5][6][7], are always expensive, poorly reproducible, time-consuming and tedious. It is a great challenge to the toxicity research, as hundreds of thousands of various pharmaceuticals are currently and regularly used.…”

Section: Introductionmentioning

confidence: 99%

A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

Shi

et al. 2015

Journal of Hazardous Materials

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Section: Introductionmentioning

confidence: 99%

A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

Shi

et al. 2015

Journal of Hazardous Materials

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“…7 It is employed in chemical screening and toxicology studies whereby behaviours, such as oviposition, 8 movement, 9 foraging, 10 avoidance, 11 and mating, 12 are assayed in response to environmental toxicants. 1,[12][13][14] Oviposition is the process of egg-laying in D. melanogaster that is dependent on responses of the fly to environmental stimuli (such as chemicals, physical conditions of the substrate, temperature, light, population density, and humidity). [15][16][17][18][19] D. melanogaster possesses sensory neurons (such as taste 20,21 and touch 22,23 sensing systems) to detect various environmental cues prior to selecting preferred sites for oviposition and egg laying.…”

mentioning

confidence: 99%

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

et al. 2015

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Drosophila melanogaster (fruit fly) is a model organism and its behaviours including oviposition (egg-laying) on agar substrates have been widely used for assessment of a variety of biological processes in flies. Physical and chemical properties of the substrate are the dominant factors affecting Drosophila's oviposition, but they have not been investigated precisely and parametrically with the existing manual approaches. As a result, many behavioral questions about Drosophila oviposition, such as the combined effects of the aforementioned substrate properties (e.g., exposure area, sugar content, and stiffness) on oviposition and viability, and their threshold values, are yet to be answered. In this paper, we have devised a simple, easily implementable, and novel methodology that allows for modification of physical and chemical composition of agar substrates in order to quantitatively study survival and oviposition of adult fruit flies in an accurate and repeatable manner. Agar substrates have been modified by surface patterning using single and hexagonally arrayed through-hole polydimethylsiloxane (PDMS) membranes with various diameters and interspacing, as well as by substrate stiffness and sugar content modification via alteration of chemical components. While pure PDMS substrates showed a significant lethal effect on flies, a 0.5 mm diameter through-hole access to agar was found to abruptly increase the survival of adult flies to more than 93%. Flies avoided ovipositing on pure PDMS and on top of substrates with 0.5 mm diameter agar exposure areas. At a hole diameter of 2 mm (i.e., 0.25% exposure area) or larger, eggs were observed to be laid predominately inside the through-holes and along the edges of the PDMS-agar interface, showing a trending increase in site selection with 4 mm (i.e., 1% exposure area threshold) demonstrating natural oviposition rates similar to pure agar. The surfacemodified agar-PDMS hybrid devices and the threshold values reported for the substrate physical and chemical conditions affecting oviposition are novel; therefore, we advocate their use for future in-depth studies of oviposition behaviour in Drosophila melanogaster with accuracy and repeatability. The technique is also useful for development of novel assays for learning and decision-making studies as well as miniaturized devices for self-assembly of eggs and embryonic developmental investigations. V C 2015 AIP Publishing LLC. [http://dx

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“…[5,6] Ağır metaller, yaygın kullanımları nedeniyle, en zararlı çevresel kimyasal kirleticilerdendir. İnsan aktiviteleri ve endüstriyel kullanımları sonucu çevreye salınan ağır metaller, doğada serbest maden cevheri formunda yer almaktadır.…”

Section: öZunclassified

Kadmiyumun erkek üreme sistemi üzerine etkisi

Güner¹,

Kavlak²

2017

Androl Bul

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GİRİŞİnfertilite, cinsel yönden aktif ve kontrasepsiyon uygulamayan bir çiftin bir yıl içerisinde gebelik elde edememesi durumudur.[1]Çiftlerin yaklaşık %25'i bir yıl içerisinde gebelik elde edememektedirler ve bunların da %15'i infertilite için medikal tedavi arayışında olup, %5'i istemelerine rağmen çocuksuz kalmaktadırlar. İnfertilite, hem erkeği hem de kadını et- ABSTRACTReproductive system functions have become more sensitive due to both endogenous factors and exogenous chemical agents, such as the increasing environmental pollution in recent times. Exposure to heavy metals which have an important place in environmental pollutants, has become a worldwide health problem leading to undesirable effects in many different systems, especially in the reproductive system. In our everyday life, the information on the effects of cadmium exposed from different sources on reproductive health effects are based on a limited number of epidemiological and experimental studies. In the literature, along with supporting experimental and occupational evidence for adverse effects of low levels of cadmium on male fertility (sperm quality and reproductive hormone levels), the limited and inadequate information on this subject is noteworthy. In order to be protected from harmful effects of cadmium, relevant experimental studies should be given weight, and the findings obtained should be examined in detail in epidemiological studies. For this reason, the aim of this review is to draw attention to the effects of cadmium on the male reproductive system. Keywords: Cadmium, male infertility, reproductive health ÖZÜreme sistemi fonksiyonları, hem endojen kaynaklı faktörlerin hem de son zamanlarda giderek artan çevre kirliliği gibi eksojen kaynaklı kimyasal ajanlar nedeniyle daha duyarlı hale gelmiştir. Çevresel kirleticilerin içinde önemli bir yere sahip olan ağır metallere maruziyet, başta üre-me sistemi olmak üzere çok farklı sistemlerde istenmeyen etkilere yol açarak büyük sağlık sorunu haline gelmiştir. Günlük yaşantımızda yer alan ağır metallerden olan kadmiyumun üreme sağlığına olan etkileri konusunda bilgi, sınırlı sayıdaki epidemiyolojik ve deneysel çalışmalara dayanmaktadır. Literatüre bakıldığında; kadmiyuma düşük seviyelerde maruz kalınması durumunda erkek fertilitesi (sperm kalitesi ve üreme hormonu düzeyleri) üzerindeki olumsuz etkilerini deneysel ve mesleki araştırmalar tarafından destekleyici kanıtlar göze çarpmakla birlikte, bu konu hakkındaki bilgilerin sınırlı ve yetersizliği dikkat çekmekte-dir. Kadmiyumun zararlı etkilerinden korunabilmek için, konuyla ilgili deneysel çalışmalara ağırlık verilmeli ve elde edilen bulgular epidemiyolojik araştırmalarda ayrıntılı olarak incelenmelidir. Bu nedenle, bu derlemenin amacı, kadmiyumun erkek üreme sistemi üzerine etkilerine dikkat çekmektir.Anahtar Kelimeler: Kadmiyum, erkek infertilitesi, üreme sağlığı kileyen bir durumdur. İstemelerine rağmen çocuk sahibi olamayan infertil çiftlerin %50'sinde erkeğe ait nedenler bulunur. Eğer infertiliteden sorumlu sadece bir faktör söz konu...

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Environmental chemical mediated male reproductive toxicity: Drosophila melanogaster as an alternate animal model

Cited by 42 publications

References 220 publications

A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

Kadmiyumun erkek üreme sistemi üzerine etkisi

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