Environmental Assessment of Aerosols, Bioaerosols, and Airborne Endotoxins in a Machining Plant

Thorne, Peter S.; DeKoster, Jeannine A.; Subramanian, P.

doi:10.1080/15428119691014288

Cited by 57 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were then centrifuged at 3000xG for 5 min to remove foam before adding 200 µl of ice-cold 99 % ethanol. The samples were applied to silica based columns 2 for the DNA cleaning procedure using the manufacturer's protocol. DNA was eluted twice using 50 µl of Qiagen elution buffer for each sample.…”

Section: Dna Extractionmentioning

confidence: 99%

“…Among the various classes of MWF, those composed of an aqueous oil emulsion with oil in high concentration (soluble oils) or lower concentration (semi-synthetic MWF) are the ones that better support the microbial growth because of their high water activity. The soluble oil MWF are composed of 3-6 % oil emulsified in water and usually contain a variety of additives, including emulsifiers, biocides, antifoaming agents, and corrosion inhibitors [2]. Even if microbicides are added to the fluids, the emulsifiable oils are propitious to microbial growth such as Gramnegative bacteria, e.g., Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

“…Even if microbicides are added to the fluids, the emulsifiable oils are propitious to microbial growth such as Gramnegative bacteria, e.g., Pseudomonas spp. [1][2][3][4]. Moreover, we have recently demonstrated that dumping, cleaning, and recharging MWF systems are of little use to rid the MWF systems of microbial problems [5].…”

Section: Introductionmentioning

confidence: 99%

“…Since their viability and culturability have little influence on immunogenic properties of bacteria and on the risk for HP development among workers, alternative methods allowing direct and accurate quantification of mycobacteria from MWF environments are to be developed. We have employed the direct count method using epifluorescence microscopy to evaluate bacterial load in MWF systems, but this approach needs to be complemented with a species-specific approach that allows detection and quantification of mycobacteria [2,5]. The major taxa that have been involved in HP outbreaks, M. chelonae and M. immunogenum, differ in their number of copies of the rRNA operon and their ability to use citrate as carbon source [13].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recovery and Quantification of Mycobacterium Immunogenum DNA from Metalworking Fluids Using Dual-Labeled Probes

Veillette

Pagé

Thorne

et al. 2005

Journal of ASTM International

View full text Add to dashboard Cite

Mycobacteria in metalworking fluids (MWF) are associated with hypersensitivity pneumonitis but are difficult to recover using culture. Quantitative PCR is a promising approach to quantify mycobacteria, but three challenges exist: mycobacterial cell lysis, high-yield DNA extraction, and removal of PCR inhibitors. We used Mycobacterium spp. primers to amplify polymorphic regions of 16S-rDNA flanked with highly conserved regions. A standard curve was constructed by cloning M. immunogenum amplification product. We developed single tube DNA extraction employing mixer mill cell disruption, enzymatic digestions (lysozyme, proteinase K) followed by a mechanical disruption, and column purification. MWF was spiked with M. immunogenum, and DNA was successfully extracted. Mycobacterial 16S-RNA genes were quantified by comparing PCR amplification detection (Cycle Threshold) from our samples with that obtained from the standard curve. Recovery and quantification of mycobacterial DNA from spiked samples approached 100 %. A rapid method for quantification of mycobacteria in MWF was demonstrated.

show abstract

Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recovery and Quantification of Mycobacterium Immunogenum DNA from Metalworking Fluids Using Dual-Labeled Probes

Veillette

Pagé

Thorne

et al. 2005

Journal of ASTM International

View full text Add to dashboard Cite

show abstract

“…There is recent evidence that chronic exposure to endotoxin-containing organic dust is associated with airway remodeling (6). Despite a clear recognition that inhaled endotoxin is an occupational hazard in agricultural settings (8,12,19,20), cotton processing (11), vegetable processing (10,31), fiberglass manufacturing (16), and metal machining environments (23,24), there are no established occupational exposure limits in the United States or Canada. This is largely due to the fact that endotoxin exposure assessment methods have not been adequately optimized and validated.…”

mentioning

confidence: 99%

Comparison of Endotoxin Exposure Assessment by Bioaerosol Impinger and Filter-Sampling Methods

Duchaine¹,

Thorne

Mériaux³

et al. 2001

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Environmental assessment data collected in two prior occupational hygiene studies of swine barns and sawmills allowed the comparison of concurrent, triplicate, side-by-side endotoxin measurements using air sampling filters and bioaerosol impingers. Endotoxin concentrations in impinger solutions and filter eluates were assayed using the Limulus amebocyte lysate assay. In sawmills, impinger sampling yielded significantly higher endotoxin concentration measurements and lower variances than filter sampling with IOM inhalable dust samplers. Analysis of variance for repeated measures showed that this association remained after controlling for other factors such as replicate, sawmill, sawmill operation, wood type, and interaction terms. Endotoxin concentrations in the swine barns were 10-fold higher on average than in sawmills. These samples demonstrated comparable endotoxin concentration estimates for impinger and filter methods although the variability was lower using the impinger method. In both occupational settings, side-by-side replicates were more uniform for the impinger samples than for the filter samples. This study demonstrates that impinger sampling is an acceptable method for quantitation of area endotoxin concentrations. Further, when sampling is performed with impingers for airborne microorganism quantitation, these same impinger solutions can yield valid endotoxin exposure estimates, negating the need for additional filter sampling.

show abstract

Aldehydes and Acetals

Bernal,

Loccisano,

Neilson

et al. 2023

Patty's Toxicology

View full text Add to dashboard Cite

Aldehydes and acetals are both naturally occurring and the result of manufacturing processes, resulting in potential human exposures in occupational settings and from the ambient air and the use of consumer products. Aldehydes and acetals have been identified as genotoxicants, carcinogens, reproductive and developmental toxicants, and irritants. However, many also demonstrate limited toxicological effects at anticipated exposure levels in the general population. Here, 64 aldehydes and acetals are cataloged by sub‐class, chemical and physical properties, toxicological effects, and regulatory standards for occupational and general population cohorts. This summary of selected aldehydes and acetals represents the range of potential toxicological effects, which may cause difficulty in utilizing read‐across to predict the toxicity of aldehydes and acetals without toxicological data.

show abstract

Environmental Assessment of Aerosols, Bioaerosols, and Airborne Endotoxins in a Machining Plant

Cited by 57 publications

References 0 publications

Recovery and Quantification of Mycobacterium Immunogenum DNA from Metalworking Fluids Using Dual-Labeled Probes

Recovery and Quantification of Mycobacterium Immunogenum DNA from Metalworking Fluids Using Dual-Labeled Probes

Comparison of Endotoxin Exposure Assessment by Bioaerosol Impinger and Filter-Sampling Methods

Aldehydes and Acetals

Contact Info

Product

Resources

About