Environmental and Economical Opportunities for the Valorisation of the Genus Atriplex: New Insights

Benzarti, Maâli; Rejeb, Kilani Ben; Debez, Ahmed; Abdelly, Chédly

doi:10.1007/978-1-4614-7028-1_16

Cited by 17 publications

(21 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Very little research has been carried out to identify the molecular mechanisms directly responsible for the specific tolerance of Atriplex species to abiotic stress [2]. We present here the analysis of a high quality cDNA library and the ESTs from A .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

Li¹,

Yu²,

Chen³

et al. 2014

IJMS

View full text Add to dashboard Cite

Little information is available on gene expression profiling of halophyte A. canescens. To elucidate the molecular mechanism for stress tolerance in A. canescens, a full-length complementary DNA library was generated from A. canescens exposed to 400 mM NaCl, and provided 343 high-quality ESTs. In an evaluation of 343 valid EST sequences in the cDNA library, 197 unigenes were assembled, among which 190 unigenes (83.1% ESTs) were identified according to their significant similarities with proteins of known functions. All the 343 EST sequences have been deposited in the dbEST GenBank under accession numbers JZ535802 to JZ536144. According to Arabidopsis MIPS functional category and GO classifications, we identified 193 unigenes of the 311 annotations EST, representing 72 non-redundant unigenes sharing similarities with genes related to the defense response. The sets of ESTs obtained provide a rich genetic resource and 17 up-regulated genes related to salt stress resistance were identified by qRT-PCR. Six of these genes may contribute crucially to earlier and later stage salt stress resistance. Additionally, among the 343 unigenes sequences, 22 simple sequence repeats (SSRs) were also identified contributing to the study of A. canescens resources.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Tolerance to salinity, drought, heavy metals and temperature are important characteristics for Atriplex species, especially tolerance to drought and salt [2]. And it is of great significance for generating tolerant crops via selective breeding or genetic engineering to elucidate its tolerance mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

Li¹,

Yu²,

Chen³

et al. 2014

IJMS

View full text Add to dashboard Cite

show abstract

“…This species is also an attractive fodder shrub for most livestock and large animals due to its high palatability as well as rich nutrition ( Peterson et al, 1987 ; Kong, 2013 ). Moreover, it is especially useful for erosion control and reclamation of marginal lands due to its extensive root system and excellent adaptability ( Benzarti et al, 2013 ; Kong, 2013 ). In 1989, A. canescens was introduced from USA to China and was widely used for soil and water conservation, sand-fixing and saline land restoration in north China ( Kong, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The Photosynthesis, Na+/K+ Homeostasis and Osmotic Adjustment of Atriplex canescens in Response to Salinity

et al. 2016

View full text Add to dashboard Cite

Atriplex canescens (fourwing saltbush) is a C4 perennial fodder shrub with excellent resistance to salinity. However, the mechanisms underlying the salt tolerance in A. canescens are poorly understood. In this study, 5-weeks-old A. canescens seedlings were treated with various concentrations of external NaCl (0–400 mM). The results showed that the growth of A. canescens seedlings was significantly stimulated by moderate salinity (100 mM NaCl) and unaffected by high salinity (200 or 400 mM NaCl). Furthermore, A. canescens seedlings showed higher photosynthetic capacity under NaCl treatments (except for 100 mM NaCl treatment) with significant increases in net photosynthetic rate and water use efficiency. Under saline conditions, the A. canescens seedlings accumulated more Na+ in either plant tissues or salt bladders, and also retained relatively constant K+ in leaf tissues and bladders by enhancing the selective transport capacity for K+ over Na+ (ST value) from stem to leaf and from leaf to bladder. External NaCl treatments on A. canescens seedlings had no adverse impact on leaf relative water content, and this resulted from lower leaf osmotic potential under the salinity conditions. The contribution of Na+ to the leaf osmotic potential (Ψs) was sharply enhanced from 2% in control plants to 49% in plants subjected to 400 mM NaCl. However, the contribution of K+ to Ψs showed a significant decrease from 34% (control) to 9% under 400 mM NaCl. Interestingly, concentrations of betaine and free proline showed significant increase in the leaves of A. canescens seedlings, these compatible solutes presented up to 12% of contribution to Ψs under high salinity. These findings suggest that, under saline environments, A. canescens is able to enhance photosynthetic capacity, increase Na+ accumulation in tissues and salt bladders, maintain relative K+ homeostasis in leaves, and use inorganic ions and compatible solutes for osmotic adjustment which may contribute to the improvement of water status in plant.

show abstract

“…The elite halophyte Atriplex canescens (four-wing saltbush), a member of the Chenopodiaceae , is indigenous in arid and semi-arid areas of western North America. A. canescens exhibits tolerance to salinity, drought, heavy metals and low temperature and the plant has been employed in phytoremediation of saline–alkali and heavy-metal contaminated soils [ 14 ]. Therefore, the plant is a source of genes that could be employed in the genetic manipulation of crops for improvements in salt, drought and low temperature stress [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…A. canescens exhibits tolerance to salinity, drought, heavy metals and low temperature and the plant has been employed in phytoremediation of saline–alkali and heavy-metal contaminated soils [ 14 ]. Therefore, the plant is a source of genes that could be employed in the genetic manipulation of crops for improvements in salt, drought and low temperature stress [ 14 ]. Yeast expression systems have been extensively used for protein functional characterization and protein production, combining advantages in rapid growth and facile genetic manipulation with the relevance of a eukaryotic expression system, such as capacity for post-translational modifications [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System

Liu

et al. 2017

IJMS

View full text Add to dashboard Cite

Plant productivity is limited by salinity stress, both in natural and agricultural systems. Identification of salt stress-related genes from halophyte can provide insights into mechanisms of salt stress tolerance in plants. Atriplex canescens is a xero-halophyte that exhibits optimum growth in the presence of 400 mM NaCl. A cDNA library derived from highly salt-treated A. canescens plants was constructed based on a yeast expression system. A total of 53 transgenic yeast clones expressing enhanced salt tolerance were selected from 105 transformants. Their plasmids were sequenced and the gene characteristics were annotated using a BLASTX search. Retransformation of yeast cells with the selected plasmids conferred salt tolerance to the resulting transformants. The expression patterns of 28 of these stress-related genes were further investigated in A. canescens leaves by quantitative reverse transcription-PCR. In this study, we provided a rapid and robust assay system for large-scale screening of genes for varied abiotic stress tolerance with high efficiency in A. canescens.

show abstract

Environmental and Economical Opportunities for the Valorisation of the Genus Atriplex: New Insights

Cited by 17 publications

References 77 publications

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

The Photosynthesis, Na+/K+ Homeostasis and Osmotic Adjustment of Atriplex canescens in Response to Salinity

Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System

Contact Info

Product

Resources

About