Environmental and Dietary Exposures Near a Major Industrial Harbour (Fos-sur-Mer, France) Identified as a Significant Pathway for PCBs and PCDD/Fs Accumulation in Residents’ Blood Serum

Jeanjean, Maxime; Goix, Sylvaine; Periot, Marine; Douib, Khaled; Dron, Julien; Étienne, Marie‐Pierre; Marchand, Philippe; Austruy, Annabelle; Revenko, G. P.; Chamaret, Philippe

doi:10.1007/s12403-021-00395-8

Cited by 11 publications

(1 citation statement)

References 50 publications

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“…11 Traditional analytical methods for human biomonitoring are mostly targeted and mass spectrometrybased, employing either liquid chromatography (LC) or gas chromatography (GC). [12][13][14][15][16][17][18][19] Such methods are very sensitive and quantitative, but can require large sample volumes and laborious sample processing steps, and ultimately reveal only a limited fraction of the chemical exposome, defined a priori by the investigators. Today there are over 350,000 chemicals in global commerce, 20 but only 5% of these have ever been analyzed in environmental media, 21 and likely fewer have been biomonitored in humans.…”

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confidence: 99%

Chemical Exposomics in Human Plasma by Lipid Removal and Large Volume Injection Gas Chromatography High-Resolution Mass Spectrometry

Xie,

Sdougkou,

Papazian

et al. 2024

Preprint

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For comprehensive chemical exposomics in blood, analytical workflows are evolving through advances in sample preparation and instrumental methods. We hypothesized that gas chromatography high-resolution mass spectrometry (GC-HRMS) workflows could be enhanced by minimizing lipid co-extractives, thereby enabling larger injection volumes and lower matrix interference for improved sensitivity and molecular discovery. A simple protocol was developed for small plasma volumes (100-200 µL) by using isohexane (H) to extract supernatants of acetonitrile-plasma (A-P). The HA-P method was quantitative for a wide range of hydrophobic multiclass target analytes (i.e. log Kow >3.0), and the extracts were free of major lipids, thereby enabling robust large-volume injections (LVI; 25 µL) in long sequences to a GC-Orbitrap HRMS. Without lipid removal, LVI was counter-productive because method sensitivity suffered from the abundant matrix signal, resulting in low ion injection times to the Orbitrap. The median method quantification limit was 0.08 ng/mL (range 0.005–4.83 ng/mL), and good accuracy was shown for a certified reference serum. Applying the method to plasma from a Swedish cohort (n=32; 100 µL), 51 of 103 target analytes were detected. Simultaneous nontarget analysis resulted in 112 structural annotations (12.8% annotation rate), and Level 1 identification was achieved for 7 of 8 substances in follow-up confirmations. The HA-P method is potentially scalable for application in cohort studies and is also compatible with many liquid-chromatography-based exposomics workflows.

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confidence: 99%

Chemical Exposomics in Human Plasma by Lipid Removal and Large Volume Injection Gas Chromatography High-Resolution Mass Spectrometry

Xie,

Sdougkou,

Papazian

et al. 2024

Preprint

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Human Biomonitoring of Polychlorinated Biphenyls (PCBs) in the Breast Milk of Colombian Mothers

Ávila

Ramírez²,

Téllez-Avila³

2022

Bull Environ Contam Toxicol

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Concentrations and transportation of metal and organochlorine pollutants in vegetables and risk assessment of human exposure in rural, urban and industrial environments (Bouches-du-Rhône, France)

Austruy¹,

Roulier²,

Angeletti

et al. 2021

Environ Sci Pollut Res

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The bioaccumulation of metals (As, Cd, Co, Cr, Cu, Ni, Pb, Sb, V, Zn, Al, Fe) and organochlorine compounds (PCDD-Fs, and PCBs) was assessed in soils and vegetables of 3 sites of contrasted anthropogenic in uence (rural and industrial-urban areas). Cultivated soils in industrial areas exhibited diffuse pollution in organochlorine pollutants (PCBs and PCDD-Fs). The pollutant levels encountered in vegetables were always lower than the regulatory or recommended values by EU. However, the contents measured in vegetables cultivated near industrialised areas were signi cantly higher than those observed in rural areas, this was notably the case for Co, Cd, Cr, Ni, Pb, V, NDL-and DL-PCB, PCDD and PCDF. The leaf pathway appeared as the main absorption pathway for many contaminants. The results suggested that population exposure to pollutants was mainly caused by the vegetable ingestion. In the vegetables and soils, the toxicity was mainly caused by the V, Co, Cd and Pb contents to which can be added As and PCDD-Fs for soils. Therefore, the proximity of vegetable crops to highly anthropised areas has led to long-term exposure of vegetables and soils to air pollutants, leading to an accumulation in the food-chain and thus a risk for human health.

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Environmental and Dietary Exposures Near a Major Industrial Harbour (Fos-sur-Mer, France) Identified as a Significant Pathway for PCBs and PCDD/Fs Accumulation in Residents’ Blood Serum

Cited by 11 publications

References 50 publications

Chemical Exposomics in Human Plasma by Lipid Removal and Large Volume Injection Gas Chromatography High-Resolution Mass Spectrometry

Chemical Exposomics in Human Plasma by Lipid Removal and Large Volume Injection Gas Chromatography High-Resolution Mass Spectrometry

Human Biomonitoring of Polychlorinated Biphenyls (PCBs) in the Breast Milk of Colombian Mothers

Concentrations and transportation of metal and organochlorine pollutants in vegetables and risk assessment of human exposure in rural, urban and industrial environments (Bouches-du-Rhône, France)

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