Environmental analysis of aliphatic carboxylic acids by ion-exclusion chromatography

Fischer, Klaus

doi:10.1016/s0003-2670(02)00204-0

Cited by 48 publications

(16 citation statements)

References 105 publications

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“…In the depths of the rhizosphere science, improved techniques are required to study roots in vivo, particularly for measuring either the low concentrations of organic acids released into in soil solution, or the temporal nature of that release and subsequent solubilisation of soil-P. On the analytical side, recent progress involves the use of capillary electrophoresis (Barbas et al 1999;Xu et al 2002), ion chromatography (Tani et al 2001), and ion exclusion chromatography (Fischer 2001), which allows detection of the nanomolar concentrations of organic anions likely released from small sections of growing root per day.…”

Section: Do We Need More Rhizosphere Research?mentioning

confidence: 99%

Progress in selected areas of rhizosphere research on P acquisition

et al. 2003

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Large reserves of P have accumulated in soils of developed countries because additions of P fertiliser to sustain agricultural production have exceeded crop removal. By contrast, in many developing countries in the tropics and subtropics, soil P reserves are gravely low and large additions are required before maintenance requirements begin to decline. In addition, the cost of P fertiliser will increase as the currently accessible deposits of high-grade phosphate rock (PR) diminish. Developing plants that efficiently tap soil P reserves and low grade PR is therefore a priority for agricultural research. For the 50th anniversary of the New Zealand Soil Science Society, this paper reviews research on P efficiency in plants, conducted by staff, students, and research associates of Massey University, in the context of other research into plant mechanisms that enhance P uptake, including effects of root geometry, mycorrhizal associations, and root-induced changes in the soil. Techniques for fractionation of soil P are highlighted. S R 0 2 1 3 0 R h i z o s p h e r e P a c q u i s i t i o n S . N . T r o l o v e e t a l .

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Section: Do We Need More Rhizosphere Research?mentioning

confidence: 99%

Progress in selected areas of rhizosphere research on P acquisition

et al. 2003

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“…Yet, the full capabilities of these relatively new stationary phases for CA separations were not fully explored. The earlier applications focused either on the low molecular weight (<C 6 ) [1,2,12,13] or some high molecular weight CAs (>C 6 ) [3,4,14–16]. …”

Section: Introductionmentioning

confidence: 99%

Limits of detection for the determination of mono- and dicarboxylic acids using gas and liquid chromatographic methods coupled with mass spectrometry

Šťávová

Beránek

Nelson

et al. 2011

Journal of Chromatography B

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The chromatographic separation and instrumental limits of detection (LODs) were obtained for a broad range of C1-C18 monocarboxylic (MCAs) and C2-C14 dicarboxylic acids (DCAs) employing either chemical derivatization followed by gas chromatography-mass spectrometry and flame ionization detection (GC-MS/FID) or direct analysis with liquid chromatography high resolution MS and tandem MS (LC-MS). Suitability, efficiency and stability of reaction products for several derivatization agents used for esterification (BF3/butanol), and trimethysilylation, including trimethylsilyl-N-N-dimethylcarbamate (TMSDMC) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) were evaluated. The lowest limits of detection for the majority of compounds below 10 pg (with the exception of acetic acid) were obtained for derivatization with BF3/butanol followed by GC-MS in the total ion current (TIC) mode. Further improvements were achieved when applying either selected ion monitoring (SIM), which decreased the LODs to 1–4 pg or a combination of SIM and TIC (SITI) (2–5 pg). GC-FID provided LODs comparable to those obtained by GC-MS TIC. Both trimethylsilylation (followed by GC-MS) and direct LC-MS/MS analysis yielded LODs of 5– 40 pg for most of the acids. For volatile acids the LODs were higher, e.g., 25 and 590 ng for TMSDMC and BSTFA derivatized formic acid, respectively whereas the LC-MS methods did not allow for the analysis of formic acid at all.

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“…MurN eluted only with 1% phosphoric acid as the mobile phase, whereas the basic amino sugars did not even elute after five hours. The separation of the ion exclusion mechanism is based on the Donnan membrane exclusion principle (Hicks, Lim, and Haas 1985;Fischer 2002). Hicks et al (1985) described ion exclusion as a process where ionic solutes pass through the interstitial volume of the resin, but are prevented from entering the pore volume of the sulfonated divenylbenzene column material, because of the Donnan membrane effects.…”

Section: Hpcec and Hpexcmentioning

confidence: 99%

Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

Indorf¹,

Bodé²,

Boeckx³

et al. 2013

Analytical Letters

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A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 lmol L À1 (HPAEC-FL) and 1.0-10.0 lmol L À1 (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 lmol L À1 ). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.

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Environmental analysis of aliphatic carboxylic acids by ion-exclusion chromatography

Cited by 48 publications

References 105 publications

Progress in selected areas of rhizosphere research on P acquisition

Progress in selected areas of rhizosphere research on P acquisition

Limits of detection for the determination of mono- and dicarboxylic acids using gas and liquid chromatographic methods coupled with mass spectrometry

Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

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