Environmental adjustments of the cooperativity in M13 phage thermal denaturation

González-Cansino, Jhoana L.; Vieyra-Eusebio, María Teresa; Vera-Robles, L. Iraís; Hernández‐Arana, Andrés

doi:10.1016/j.tca.2018.12.010

Cited by 7 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To enable efficient (photo)electrochemical hydrogen production, superaerophobic hydrogels were readily formed on a target electrode by cross-linking the M13 virus. This virus was chosen as the building block for the hydrogels because of its inherent nanofibrillar structure, hydrophilicity (30), and high stability under various conditions (31)(32)(33)(34). At ambient temperature and pressure, the M13 virus readily forms a highly porous hydrogel through a condensation reaction with glutaraldehyde ( Fig.…”

Section: Preparation Of Porous Hydrogelsmentioning

confidence: 99%

Superaerophobic hydrogels for enhanced electrochemical and photoelectrochemical hydrogen production

et al. 2020

View full text Add to dashboard Cite

The efficient removal of gas bubbles in (photo)electrochemical gas evolution reactions is an important but underexplored issue. Conventionally, researchers have attempted to impart bubble-repellent properties (so-called superaerophobicity) to electrodes by controlling their microstructures. However, conventional approaches have limitations, as they are material specific, difficult to scale up, possibly detrimental to the electrodes’ catalytic activity and stability, and incompatible with photoelectrochemical applications. To address these issues, we report a simple strategy for the realization of superaerophobic (photo)electrodes via the deposition of hydrogels on a desired electrode surface. For a proof-of-concept demonstration, we deposited a transparent hydrogel assembled from M13 virus onto (photo)electrodes for a hydrogen evolution reaction. The hydrogel overlayer facilitated the elimination of hydrogen bubbles and substantially improved the (photo)electrodes’ performances by maintaining high catalytic activity and minimizing the concentration overpotential. This study can contribute to the practical application of various types of (photo)electrochemical gas evolution reactions.

show abstract

Section: Preparation Of Porous Hydrogelsmentioning

confidence: 99%

Superaerophobic hydrogels for enhanced electrochemical and photoelectrochemical hydrogen production

et al. 2020

View full text Add to dashboard Cite

show abstract

“…34 We used the M13 bacteriophage as a hydrogel building block due to its unique features, such as inherent nanobrous structure, hydrophilicity, and high stability. [35][36][37] Upon addition of glutaraldehyde, M13 nanobers readily formed the hydrogel via the Schiff-base condensation reaction between glutaraldehyde and rich amine groups on their surface. NiF can be embedded with hydrogels via capillary lling of M13 solution within its pores and subsequent dipping in glutaraldehyde solution.…”

Section: Resultsmentioning

confidence: 99%

Nanofibrillar hydrogels outperform Pt/C for hydrogen evolution reactions under high-current conditions

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The thermally denatured M13KO7 showed the same spectral pattern as the normal M13KO7, with a 5,239 m / z peak of the major coat protein PVIII. Based on previous research about differential scanning calorimetry profile of M13 phage, heat capacity of M13(Cp) consists of maximum at a 93°C and a shoulder extending from 97°C to almost 110°C, causing irreversible denaturation ( 26 ).…”

Section: Resultsmentioning

confidence: 99%

M13KO7 bacteriophage enables Potato Virus Y detection

Seo,

Cho,

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

Potato virus Y (PVY) is one of the foremost plant viruses affecting potato plants and production. Many diagnostic and detection techniques are available to detect and prevent the spread of this virus. Serological analyses, such as enzyme-linked immunosorbent assay (ELISA), are widely used in laboratories as well as in the field. In addition, recently, serological methods using whole or recombinant antibodies have been developed. However, these antibodies are unstable under certain chemical conditions and can be easily degraded by exposure to enzymes. M13KO7, a derivative of bacteriophage M13, with a filamentous structure similar to PVY, was used for antibody screening. We found that PVY and “bald” M13KO7 had binding activity similar to that of antigen-antibody in ELISA analyses. The binding interactions between PVY and M13KO7 were highly specific. A new method for detecting PVY using ELISA analysis with M13KO7 was developed in this study. These results provide new insights into two virus-binding interactions and novel systems for the detection of plant viruses. IMPORTANCE In this study, we confirmed the binding of M13KO7 to Potato virus Y (PVY) using enzyme-linked immunosorbent assay. M13KO7 is a “bald” bacteriophage in which no recombinant antibody is displayed. M13KO7 is easy to propagate by using Escherichia coli , making this method more reasonable in economic perspective. Based on this study, we suggest that M13KO7 detection system has applicability as a novel biological tool for the detection of PVY

show abstract

Environmental adjustments of the cooperativity in M13 phage thermal denaturation

Cited by 7 publications

References 42 publications

Superaerophobic hydrogels for enhanced electrochemical and photoelectrochemical hydrogen production

Superaerophobic hydrogels for enhanced electrochemical and photoelectrochemical hydrogen production

Nanofibrillar hydrogels outperform Pt/C for hydrogen evolution reactions under high-current conditions

M13KO7 bacteriophage enables Potato Virus Y detection

Contact Info

Product

Resources

About