Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying

Desmond, C.; Stanton, Catherine; Fitzgerald, Gerald F.; Collins, Kevin J.; Ross, R. Paul

doi:10.1016/s0958-6946(02)00040-7

Cited by 126 publications

(119 citation statements)

References 23 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…It has been revealed that bacteria respond to changes in their immediate surroundings by a metabolic reprogramming which leads to a cellular state of enhanced resistance. For instance, pre-adaptation of L. paracasei NFBC 338 by exposure to 0.3 M NaCl resulted in significant resistance to heat stress associated with spray drying (at outlet temperatures of 95-100°C) in comparison with non-adapted control cells (33.46± 2.3 % versus 8.27±4.42 % survival, respectively) (Desmond et al, 2001). It has been reported that the viability of the heatadapted L. paracasei NFBC 338 in reconstituted skim milk was enhanced 18-fold during spray drying at outlet temperatures of 95-105°C (Desmond et al, 2001).…”

Section: Important Parameters Affecting Microencapsulationmentioning

confidence: 99%

“…For instance, pre-adaptation of L. paracasei NFBC 338 by exposure to 0.3 M NaCl resulted in significant resistance to heat stress associated with spray drying (at outlet temperatures of 95-100°C) in comparison with non-adapted control cells (33.46± 2.3 % versus 8.27±4.42 % survival, respectively) (Desmond et al, 2001). It has been reported that the viability of the heatadapted L. paracasei NFBC 338 in reconstituted skim milk was enhanced 18-fold during spray drying at outlet temperatures of 95-105°C (Desmond et al, 2001). It is well known reconstituted skim-milk/Raftilose® P95 and reconstituted that the stress responses of bacterial cultures depend on the growth phase.…”

Section: Important Parameters Affecting Microencapsulationmentioning

confidence: 99%

See 1 more Smart Citation

Technology and potential applications of probiotic encapsulation in fermented milk products

2014

View full text Add to dashboard Cite

Fermented milk products containing probiotics and prebiotics can be used in management, prevention and treatment of some important diseases (e.g., intestinal-and immune-associated diseases). Microencapsulation has been used as an efficient method for improving the viability of probiotics in fermented milks and gastrointestinal tract. Microencapsulation of probiotic bacterial cells provides shelter against adverse conditions during processing, storage and gastrointestinal passage. Important challenges in the field include survival of probiotics during microencapsulation, stability of microencapsulated probiotics in fermented milks, sensory quality of fermented milks with microencapsulated probiotics, and efficacy of microencapsulation to deliver probiotics and their controlled or targeted release in the gastrointestinal tract. This study reviews the current knowledge, and the future prospects and challenges of microencapsulation of probiotics used in fermented milk products. In addition, the influence of microencapsulation on probiotics viability and survival is reviewed.

show abstract

Section: Important Parameters Affecting Microencapsulationmentioning

confidence: 99%

Section: Important Parameters Affecting Microencapsulationmentioning

confidence: 99%

Technology and potential applications of probiotic encapsulation in fermented milk products

2014

View full text Add to dashboard Cite

show abstract

“…This definition was later endorsed by the International Scientific Association for Probiotics and Prebiotics (ISAPP) and is currently the most accepted definition of probiotics by scientists worldwide (Reid, 2006). Probiotic food cultures have become popular due to appreciation of their contribution to good health (Desmond et al, 2002). In probiotic therapy, these beneficial microorganisms are ingested and thereby introduced to the intestinal microflora intentionally.…”

mentioning

confidence: 99%

Probiotics - What They Are, Their Benefits and Challenges

Thantsha¹,

Mamvura²,

Booyens³

2012

New Advances in the Basic and Clinical Gastroenterology

View full text Add to dashboard Cite

“…Previous published attempts to spray dry bacteria, although successful at producing viable material, have not yet succeeded in producing highly viable and room-temperature stable bacteria, of any kind, nor have they been applied to BCG. Desmond et al (9) showed that Lactobacillus paracasei could be spray dried with gum acacia to increase survival of bacteria 1,000-fold when stored for 4 weeks at 15°C, and viability dropped even further by 99.9% over 4 weeks at 30°C. Similar losses of viability at room temperature were seen in this study for spray dried Spirulina platensis; survival dropped to 50% after only 6 days of storage at 30°C and dropped to 0% after 3 days of storage at 45°C.…”

mentioning

confidence: 99%

Drying a tuberculosis vaccine without freezing

Wong

Sampson

Germishuizen

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

With the increasing incidence of tuberculosis and drug resistant disease in developing countries due to HIV/AIDS, there is a need for vaccines that are more effective than the present bacillus CalmetteGué rin (BCG) vaccine. We demonstrate that BCG vaccine can be dried without traditional freezing and maintained with remarkable refrigerated and room-temperature stability for months through spray drying. Studies with a model Mycobacterium (Mycobacterium smegmatis) revealed that by removing salts and cryoprotectant (e.g., glycerol) from bacterial suspensions, the significant osmotic pressures that are normally produced on bacterial membranes through droplet drying can be reduced sufficiently to minimize loss of viability on drying by up to 2 orders of magnitude. By placing the bacteria in a matrix of leucine, high-yield, freeflowing, ''vial-fillable'' powders of bacteria (including M. smegmatis and M. bovis BCG) can be produced. These powders show relatively minor losses of activity after maintenance at 4°C and 25°C up to and beyond 4 months. Comparisons with lyophilized material prepared both with the same formulation and with a commercial formulation reveal that the spray-dried BCG has better overall viability on drying.aerosolization ͉ bacillus Calmette-Gué rin ͉ spray drying ͉ dry powder ͉ mycobacterium B acillus Calmette-Guérin (BCG), the most widely administered childhood vaccine in the world with 100 million infant administrations annually (1), is presently delivered by needle injection, requires refrigerated storage, and has shown in different parts of the world variable degrees of protection against tuberculosis (TB) ranging from 0% to 80% (2). Given the increasing global burden of TB, its coupling to the HIV/AIDS epidemic, and the emerging problem of drug resistance, there is a clear need for alternatives to the traditional BCG vaccine and current treatment strategies (2, 3). An optimal new vaccine would obviate needle injection, not require refrigerated storage, and provide a safe and more consistent degree of protection.Current commercial BCG vaccine preparations are filled as bacterial suspensions in vials, dried through lyophilization, and stabilized through refrigeration for up to 1 year on the shelf (4). Lyophilization, or freeze-drying, however, is known to produce significant losses of BCG viability after freezing (5). Moreover, lyophilized BCG fails to exhibit satisfactory shelf stability at room temperature, a major liability for transport and distribution, particularly in low-income environments, where the TB epidemic is most seriously concentrated (4, 6). Most importantly for our work, lyophilized BCG does not exhibit a particle form conducive for potential noninjectable delivery, notably through an aerosol route.In the search for a more effective vaccine against TB, spray drying provides an attractive alternative to lyophilization. It is less costly to operate (6), can be scaled up in sterile conditions, avoids cell freezing (7), and has the potential to be inhaled (8). Previous published att...

show abstract

Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying

Cited by 126 publications

References 23 publications

Technology and potential applications of probiotic encapsulation in fermented milk products

Technology and potential applications of probiotic encapsulation in fermented milk products

Probiotics - What They Are, Their Benefits and Challenges

Drying a tuberculosis vaccine without freezing

Contact Info

Product

Resources

About