Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers

Hu, Xiongwei; Zhang, Jian; Zhou, Yu; Xie, Yunchang; He, Haisheng; Qi, Jianping; Dong, Xiaochun; Lü, Yi; Zhao, Weili; Wu, Wei

doi:10.1016/j.nano.2015.06.013

Cited by 103 publications

(113 citation statements)

References 46 publications

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“…Recently, our group introduced a type of fluorescent probes bearing a BODIPY or aza‐BODIPY structure (Figure S1, Supporting Information) . The distinct feature of these probes is the aggregation‐caused quenching (ACQ) effects.…”

Section: Introductionmentioning

confidence: 99%

“…The distinct feature of these probes is the aggregation‐caused quenching (ACQ) effects. The probe molecule emits near‐infrared (NIR) fluorescence, while being instantly and completely quenched upon contacting with water due to formation of self‐aggregated clusters through intermolecular π–π stacking . The ACQ probes were regarded as an innovative bioimaging tool to identify intact nanoparticles .…”

Section: Introductionmentioning

confidence: 99%

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Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Xie

et al. 2018

Adv Healthcare Materials

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The limited information on biological fate impedes the development of more efficient polymeric nanoparticles for oral delivery of bio‐macromolecules. In this study, the in vivo fate as well as the trans‐epithelia transport of polycaprolactone (PCL) nanoparticles is explored by labeling with aggregation‐caused quenching probes, which is capable of identifying intact nanoparticles. Live imaging and confocal laser scan microscopy confirm size‐dependent absorption of PCL nanoparticles. In general, reducing particle size favors a faster and more oral absorption. Nanoparticles larger than 200 nm, such as 600 and 2000 nm, cannot be efficiently transported across the intestinal membrane. The absorbed nanoparticles (50 and 200 nm) mainly accumulate in the liver. Lymph may be the main absorption route for PCL nanoparticles, transporting 2.39 ± 1.81% and 0.98 ± 0.58% of administered 50 and 200 nm nanoparticles, respectively. Cellular uptake and transportation of PCL nanoparticles are also size dependent. Both enterocytes and M cells mediated transcytosis are involved in the transport of 50 nm PCL nanoparticles, while the M cell pathway is dominative for other nanoparticles. In conclusion, the study provides a valuable tool for bioimaging of intact polymeric nanoparticles as well as solid evidence supporting size‐dependent translocation of the nanoparticles via oral delivery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Xie

et al. 2018

Adv Healthcare Materials

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“…Recently, our group developed a series of near-infrared (NIR) fluorescent probes with a BODIPY or aza-BODIPY structure to track the in vivo fate of integral nanoparticles [42–44]. The distinct feature of these probes is the sensitive aggregation-caused quenching (ACQ) effects upon contact with water through π-π stacking.…”

Section: Introductionmentioning

confidence: 99%

Size-dependent penetration of nanoemulsions into epidermis and hair follicles: implications for transdermal delivery and immunization

et al. 2017

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Nanoemulsions have been widely applied to dermal and transdermal drug delivery. However, whether and to what depth the integral nanoemulsions can permeate into the skin is not fully understood. In this study, an environment-responsive dye, P4, was loaded into nanoemulsions to track the transdermal translocation of the nanocarriers, while coumarin-6 was embedded to represent the cargoes. Particle size has great effects on the transdermal transportation of nanoemulsions. Integral nanoemulsions with particle size of 80 nm can diffuse into but not penetrate the viable epidermis. Instead, these nanoemulsions can efficiently fill the whole hair follicle canals and reach as deep as 588 μm underneath the dermal surfaces. The cargos are released from the nanoemulsions and diffuse into the surrounding dermal tissues. On the contrary, big nanoemulsions, with mean particle size of 500 nm, cannot penetrate the stratum corneum and can only migrate along the hair follicle canals. Nanoemulsions with median size, e.g. 200 nm, show moderate transdermal permeation effects among the three-size nanoemulsions. In addition, colocalization between nanoemulsions and immunofluorescence labeled antigen-presenting cells was observed in the epidermis and the hair follicles, implying possible capture of nanoemulsions by these cells. In conclusion, nanoemulsions are advantageous for transdermal delivery and potential in transcutaneous immunization.

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“…After oral administration, SLNs cannot be absorbed in their intact form but could be lipolysed in the gastrointestinal tract. 7,8 During lipolysis, triglycerides are regularly digested into monoglycerides and fatty acids. The extent and rate of lipolysis are influenced by the gastric emptying rate, presence of lipolysis inhibitors and the percentage of oil and fat inherent in the body; the monoglycerides and fatty acids form mixed micelles through self-assembly with phospholipids and bile salts secreted in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, most of the drug encapsulated in nanoparticles is transferred to the epithelial cells through the unstirred water layer during the lipolysis process and is then absorbed by the intestinal epithelial cells in the form of mixed micelles or free molecules by passive diffusion, which leads to the enhancement of oral bioavailability. [7][8][9][10] Certainly, SLNs can also improve the bioavailability of insoluble drugs by other routes, including improving the dissolution process of nonaqueous drugs to increase the latter's solubility as well as promoting the drug absorption, transport and distribution due to their nanometer-scale particle size. 11 Moreover, SLNs also have a variety of advantages, including nontoxic side effects, sustained and controlled release, targeted delivery and beneficial large-scale production, as well as improving the stability of unstable substances.…”

Section: Introductionmentioning

confidence: 99%

Development of solid lipid nanoparticles containing total flavonoid extract from<em> Dracocephalum moldavica</em> L. and their therapeutic effect against myocardial ischemia–reperfusion injury in rats

Tan¹,

He²,

Wen³

et al. 2017

IJN

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Total flavonoid extract from Dracocephalum moldavica L. (TFDM) contains effective components of D. moldavica L. that have myocardial protective function. However, the cardioprotection function of TFDM is undesirable due to its poor solubility. In order to improve the solubility and efficacy of TFDM, we developed TFDM-loaded solid lipid nanoparticles (TFDM-SLNs) and optimized the formulation of TFDM-SLNs using central composite design and response surface methodology. The physicochemical properties of TFDM-SLNs were characterized, and the pharmacodynamics was investigated using the myocardial ischemia–reperfusion injury model in rats. The nanoparticles of optimal formulation for TFDM-SLNs were spherical in shape with the average particle size of 104.83 nm and had a uniform size distribution with the polydispersity index value of 0.201. TFDM-SLNs also had a negative zeta potential of −28.7 mV to ensure the stability of the TFDM-SLNs emulsion system. The results of pharmacodynamics demonstrated that both TFDM and TFDM-SLN groups afforded myocardial protection, and the protective effect of TFDM-SLNs was significantly superior to that of TFDM alone, based on the infarct area, histopathological examination, cardiac enzyme levels and inflammatory factors in serum. Due to the optimal quality and the better myocardial protective effect, TFDM-SLNs are expected to become a safe and effective nanocarrier for the oral delivery of TFDM.

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Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers

Cited by 103 publications

References 46 publications

Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Size-dependent penetration of nanoemulsions into epidermis and hair follicles: implications for transdermal delivery and immunization

Development of solid lipid nanoparticles containing total flavonoid extract from<em> Dracocephalum moldavica</em> L. and their therapeutic effect against myocardial ischemia–reperfusion injury in rats

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Environment-responsive aza-BODIPY dyes quenching in water as potential probes to visualize the in vivo fate of lipid-based nanocarriers

Cited by 103 publications

References 46 publications

Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Bioimaging of Intact Polycaprolactone Nanoparticles Using Aggregation‐Caused Quenching Probes: Size‐Dependent Translocation via Oral Delivery

Size-dependent penetration of nanoemulsions into epidermis and hair follicles: implications for transdermal delivery and immunization

Development of solid lipid nanoparticles containing total flavonoid extract from<em> Dracocephalum moldavica</em> L. and their therapeutic effect against myocardial ischemia&ndash;reperfusion injury in rats

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Product

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Development of solid lipid nanoparticles containing total flavonoid extract from<em> Dracocephalum moldavica</em> L. and their therapeutic effect against myocardial ischemia–reperfusion injury in rats