Environment-induced conformational and functional changes of l-2-haloacid dehalogenase

Wang, Yayue; Cao, Xupeng; Feng, Yanbin; Xue, Song

doi:10.1016/j.jbiosc.2015.09.008

Cited by 4 publications

(4 citation statements)

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“…Hpk6, hpk7, and hpk11 proteins, belonging to histidine protein kinase (HPK), have a regulatory function in the synthesis of plantaricins and are therefore crucial to the strains [61]. The deh gene encodes an L-2-haloacid dehalogenase, an enzyme that degrades halogenated compounds present in drugs and environmental pollutants such as chlorobenzene, chlorocyclohexane, chloroalkane, and chloroalkene [62]. This enzyme presents applications in chemical industries, bioremediation, and sustainable chemistry [60, 61].…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis ofLactobacillus plantarum: An Overview

Evanovich

Mattos

Guerreiro

2019

International Journal of Genomics

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Background. Lactobacillus plantarum is widely used in the manufacture of dairy products, fermented foods, and bacteriocins. The genomes of the strains contain multiple genes which may have been acquired by horizontal gene transfer. Many of these genes are important for the regulation, metabolism, and transport of various sugars; however, other genes may carry and spread virulence and antibiotic resistance determinants. In this way, monitoring these genomes is essential to the manufacture of food. In this study, we aim to provide an overview of the genomic properties of L. plantarum based on approaches of comparative genomics. Results. The finding of the current study indicates that the core genome of L. plantarum presents 1425 protein-coding genes and is mostly related to the metabolic process. The accessory genome has on average 1320 genes that encodes protein involved in processes as the formation of bacteriocins, degradation of halogen, arsenic detoxification, and nisin resistance. Most of the strains show an ancestral synteny, similar to the one described in the genomes of L. pentosus KCA1 and L. plantarum WCFS1. The lifestyle island analyses did not show a pattern of arrangement or gene content according to habitat. Conclusions. Our results suggest that there is a high rate of transfer of genetic material between the strains. We did not identify any virulence factors and antibiotic resistance genes on the genomes. Thus, the strains may be useful for the biotechnology, bioremediation, and production of bacteriocins. The potential applications are, however, restricted to particular strains.

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Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis ofLactobacillus plantarum: An Overview

Evanovich

Mattos

Guerreiro

2019

International Journal of Genomics

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“…L-DEX specifically acts on L-2-haloalkanoic acids to produce D-2-hydroxyalkanoic acids. These enzymes are widespread in nature and their biochemical characteristics and structures have been studied extensively (Satpathy et al, 2016;Wang et al, 2016;Adamu et al, 2020). So far, the three-dimensional (3D) structures of specific L-DEXs and their substrate complexes have been analyzed, including L-DEX YL from Pseudomonas sp.…”

Section: Structural Characteristics and Catalytic Mechanismmentioning

confidence: 99%

Mini Review: Advances in 2-Haloacid Dehalogenases

et al. 2021

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The 2-haloacid dehalogenases (EC 3.8.1.X) are industrially important enzymes that catalyze the cleavage of carbon–halogen bonds in 2-haloalkanoic acids, releasing halogen ions and producing corresponding 2-hydroxyl acids. These enzymes are of particular interest in environmental remediation and environmentally friendly synthesis of optically pure chiral compounds due to their ability to degrade a wide range of halogenated compounds with astonishing efficiency for enantiomer resolution. The 2-haloacid dehalogenases have been extensively studied with regard to their biochemical characterization, protein crystal structures, and catalytic mechanisms. This paper comprehensively reviews the source of isolation, classification, protein structures, reaction mechanisms, biochemical properties, and application of 2-haloacid dehalogenases; current trends and avenues for further development have also been included.

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“…The aggregation of proteins often changes their secondary structure, for example in amyloid formation [ 15 , 16 ]. Circular dichroism (CD) and Fourier transform infrared (FTIR) spectrometry are effective tools for the analysis of protein secondary structures to examine the mechanisms of protein aggregation and the change of protein activity [ [17] , [18] , [19] , [20] , [21] , [22] ]. For example, the secondary structure of amyloid-β 1-42 changes from a soluble monomer with a random coil form to an insoluble aggregate with a β-sheet structure, which can be monitored by the CD spectra [ 23 ].…”

Section: Introductionmentioning

confidence: 99%