Envelope-Dependent, Cyclophilin-Independent Effects of Glycosaminoglycans on Human Immunodeficiency Virus Type 1 Attachment and Infection

Zhang, Yijun; Hatziioannou, Théodora; Zang, Trinity; Braaten, Douglas; Luban, Jeremy; Goff, Stephen P.; Bieniasz, Paul D.

doi:10.1128/jvi.76.12.6332-6343.2002

Cited by 103 publications

(103 citation statements)

References 41 publications

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“…This result shows that the observed phenotypes are not specific for HeLaP4 cells. SVT experiments with the 745/X4 cell line (CD4+/HSPG-), which lacks HSPG due to a defect in the xylosyl-transferase gene (Zhang et al 2002), confirmed the role of HSPG in VLP immobilization (Fig. 2d).…”

Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 53%

“…Furthermore, stable attachment of complete VLPs was recovered upon prolonged incubation ([1 h), most likely due to newly synthesized HSPG (data not shown). These results were further supported by using a series of CHO-derived cell lines, which either contained or lacked the HIV-1 receptor (CD4) and the relevant co-receptor (CXCR4), and either contained or lacked HSPG (Zhang et al 2002). SVT experiments using the HIV-1 infectable CHO derived cell line K1/X4 (CD4+/HSPG+), shown in Fig.…”

Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 67%

“…1a. HeLa cells, HeLaP4 cells [carrying CD4 and co-receptors; (Charneau et al 1994)] or CD4-positive or negative CHO cell derivatives (Zhang et al 2002) were incubated with VLPs in CO 2 independent medium (Invitrogen) at 37°C and illuminated in a wide-field geometry using both 476 nm from an argon-ion laser (Coherent Inc., Sabre Innova) and 532 nm light from a continuous-wave, frequency-doubled Nd:YAG laser (Soliton GmbH, DLSOS). The excitation beams were overlapped with a dichroic mirror (AHF Analysentechnik Q570LP).…”

Section: Multi-color Single-virus Tracing Setupmentioning

confidence: 99%

“…HSPG is clearly not required for HIV-1 infection of the target cells used in this study, and heparinase treatment caused only a mild reduction in infectivity (Lampe et al 2007). However, the effect of HSPG on HIV infectivity may vary depending on the properties of the viral envelope (Zhang et al 2002) and possibly also on the host cell.…”

Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 99%

“…In our initial live-cell imaging studies using these VLPs, we had observed different types of particle behavior: free diffusion, brief touches with the plasma membrane, as well as a significant percentage of particles that displayed a rapid, presumably non-productive, immobilization at the plasma membrane in an apparently heparan sulfate dependent manner (Lampe et al 2007). Heparan sulfate has been reported to be involved in HIV-1 cell attachment and to be of functional importance in some types of host cells, but the overall role of heparan sulfate for HIV-1 infection is not entirely clear (Bobardt et al 2003;Moulard et al 2000;Parren et al 1998;Ugolini et al 1999;Zhang et al 2002). In the continuative study presented here, we had made use of the strengths of SVT to investigate the interactions of HIV-1 occurring at the plasma membrane in a quantitative manner.…”

Section: Introductionmentioning

confidence: 99%

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HIV-1–cellular interactions analyzed by single virus tracing

et al. 2008

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Single virus tracing (SVT) allows the direct investigation of the entry pathway of viruses into living cells. Using fluorescently labeled virus-like particles (VLPs) and SVT, we have studied the interaction between human immunodeficiency virus type 1 (HIV-1) and the plasma membrane of living cells. From the trajectories of freely diffusing VLPs in solution, we established that the particle preparation was homogeneous and the particles had a hydrodynamic radius of 86 ± 5 nm, consistent with the size of single HI viruses. The VLPs that come in contact with the cell surface either become immobilized or rapidly dissociate from the cell surface. The fraction of virions that become immobilized on the plasma membrane correlates with the surface heparan sulfate linked proteoglycans (HSPG) concentration of the cell line tested. The particles that are not immobilized make an average of 1.5 contacts with the cell surface before diffusing away. For most cell lines investigated, the contact duration follows an exponential distribution with a lifetime between 20 and 50 ms depending on the cell type.

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Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 53%

Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 67%

Section: Multi-color Single-virus Tracing Setupmentioning

confidence: 99%

Section: Rapid Immobilization Of Vlps Depends On Heparan Sulfatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HIV-1–cellular interactions analyzed by single virus tracing

et al. 2008

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Potential roles of cellular proteins in HIV‐1

Ott

2002

Reviews in Medical Virology

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Human immunodeficiency virus type-1 particles contain host proteins, both on their surface and interior. This review summarises the cellular proteins found in these virions and covers some of their potential roles in the viral life cycle and pathogenesis. For most proteins studied, their role and function are either unknown or in the hypothesis stage. This reflects the relatively recent emphasis given to these proteins by the HIV-1 field as well as the incomplete understanding of their function in the cell. The study of cellular proteins in HIV-1 promises to help us better understand the interaction of this virus with the cell, the immune system, and the whole human host as well as to shed light on the nature of AIDS and suggest more targets for therapeutic intervention. Finally, many of the cell systems themselves are still poorly understood. The extensive study of HIV-1 has already brought increased attention to the fields of immunology and vaccine science and, in the same way, might assist our understanding of the cellular pathways themselves.

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Cellular proteins detected in HIV‐1

Ott

2008

Reviews in Medical Virology

114

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It has been known for some time that HIV-1 virions contain cellular proteins in addition to proteins encoded by the viral genome. Recent studies have vastly increased the number of host proteins detected in HIV-1. This review summarises the current findings on several cellular proteins present in these virions, including some functional studies on their potential roles in the viral replication cycle and pathogenesis. Because retroviruses require extensive assistance from host proteins and pathways, the data from biochemical characterisations of HIV-1 serve as an important starting point for understanding the role of cellular proteins that act in or influence the biology of HIV-1. Additionally, a better understanding of the interactions between cellular proteins and viral components might provide more targets for anti-HIV therapeutic intervention and provide for a better understanding of how HIV-1 alters the immune system. The extensive study of HIV-1 has already brought new insights to the fields of immunology and vaccine science. In the same way, knowledge of viral--cellular protein interactions might assist our understanding of important cellular pathways.

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Envelope-Dependent, Cyclophilin-Independent Effects of Glycosaminoglycans on Human Immunodeficiency Virus Type 1 Attachment and Infection

Cited by 103 publications

References 41 publications

HIV-1–cellular interactions analyzed by single virus tracing

HIV-1–cellular interactions analyzed by single virus tracing

Potential roles of cellular proteins in HIV‐1

Cellular proteins detected in HIV‐1

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