Enumeration of Immunoglobulin-Bearing Lymphocytes in Whole Peripheral Blood

Abstract

tritiated water as a diffusible indicator. This method requires extensive arterial sampling and time-consuming laboratory procedures for liquid scintillation counting. Besides, it does not provide information on the regional content of ELW. Regional information can be obtained by using gamma emitting tracers. Using the cyclotron produced 1 5 0 labelled water we demonstrated the feasibility of estimating ELW by external counting in the dog. The technique has now been extended to man. It is relatively non-invasive, requiring only central venous injection of isotopes (Il3In as a non-diffusible intravascular indicator and Hz 150 as a diffusible indicator) and external recording by means of. probes collimated over the chest. The total absorbed radiation dose is 0-3 rads. With this technique, information on regional blood flow, blood volume, and extravascular water volume in the lung can be readily obtained. In particular, regional ELW can be obtained expressed either in ml/ml of blood volume or in ml/ml of tissue volume.Normal subjects and patients with chronically raised left atrial pressure have been studied in the supine position. ELW was elevated above the normal range in all patients, although some of them had no radiological evidence of interstitial oedema. This increase in patients was more marked at the lung base, in contrast to normal subjects where the distribution of ELW in the supine position was uniform from apex to base. This technique is suitable to follow the sequence of regional accumulation of ELW as well as its relationship with regional blood flow and volume in patients with acute. left heart failure. 32.

The selective localization of B lymphocytes in the spleen and the role of complement receptors

“…Determination of the total number of cells localizing in the spleen showed no differences between control and CoF-treated animals (Table 3) in accordance with the results obtained by Pepys et al [28] using transfer of radiolabeled cells after CoF treatment. This suggests that the observed differences in localization in the spleens of CoF-treated animals are mainly due to changes in the relative distribution of T and B cells.…”

Lymphocyte Populations in the Peripheral Blood from Patients with IgA Nephropathy

“…Their level was comparable to that found by the Japanese group in their controls [19,20], and higher than that found in normal subjects by other groups [27,28], Serum IgA, mg/100ml < 350 < 350 Fig. 2.…”

Immune Pancytopenia

“…Using blood from normal individuals, this method separates the T-cells and the null cells from total mononuclear cells with less than I % B-cell contamination. To ascertain whether the ATG and complement selectively destroyed the T-cells, the total mononuclear cells separated by Ficoll-Hypaque (Bo yum, 1968) were counted and the percentage of T-cells and B-cells was determined (Jondal et al, 1972;Pepys et al, 1976). After removal of the rosette-forming cells, the cell suspension, containing mostly the null cells, was placed on a glass petri dish for 0.5 h at 37°C to remove the adherent cells.…”

“…After incubation, the cells were washed and suspended in appropriate medium for myeloid and erythroid cultures. To ascertain whether the ATG and complement selectively destroyed the T-cells, the total mononuclear cells separated by Ficoll-Hypaque (Bo yum, 1968) were counted and the percentage of T-cells and B-cells was determined (Jondal et al, 1972;Pepys et al, 1976). The percentage of these cells were also determined in a duplicate sample of the mononuclear cells after treatment with ATG and complement.…”