Enumeration, Effectiveness, and pH Resistance of Rhizobium meliloti Populations in Oregon Soils

Barber, Lynn E

doi:10.2136/sssaj1980.03615995004400030020x

Cited by 23 publications

(18 citation statements)

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“…The highest proportion of effective strains in the soils characterized by neutral pH and high content of the major nutrients (with respect to acid and poor soils) was demonstrated previously for R. leguminosarum bv. trifolii (Jones and Burrows, 1969;Masterson, 1968;Patel and Lambert, 1985) and R. meliloti (Barber, 1980).…”

Section: Increase In the Pea Yield And N Accumulation After Inoculatimentioning

confidence: 99%

Selection of Rhizobium leguminosarum bv. viceae strains for inoculation of Pisum sativum L. cultivars: Analysis of symbiotic efficiency and nodulation competitiveness

Фесенко¹,

Проворов

Orlova³

et al. 1995

Plant Soil

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From an analysis of 481 Rhizobium leguminosarum bv. viceae strains with 7 pea cultivars in pot and field experiments, we demonstrated that effective strains could be isolated from a rich medium-acid grey forest soil of the Or61 area (Central region of the European part of Russia) but not from a poor acid podzolic soil of the St. Petersburg area (North-West Russia). The proportion of the isolates significantly increasing N accumulation in pea plants (10.2%) is higher than that of strains increasing the shoot dry mass (4.6%) in the pot experiments. The mean values of the increase for N accumulation (33.8%) upon inoculation are also higher than for shoot mass (27.0%) in these experiments. N accumulation in the inoculated pea plants in the pot experiments was significantly correlated with seed yield and seed N accumulation in field experiments, while for shoot dry mass these correlations were either weak or not significant. Two-factor analysis of variance demonstrated that the contribution of plant cultivars to the variation of the major symbiotic efficiency parameters is higher (30.8-31.6%) and contributions of cultivar-strain specificity is lower (5.4-8.8%) than the contributions of strain genotypes (13.4-14.9%). We identified an ineffective R. leguminosarum bv. viceae strain 50 which can be used as a tester for assessing the nodulation competitiveness of the effective strains by an indirect method (analysis of dry mass and N accumulation in pea plants inoculated with the mixture of the tested effective strains and the tester strain). The relative competitive ability (RCA) determined by this method was 75.7-82.8% for strain 52 but only 10.5-13.8% for strain 250a; this difference was confirmed by a direct method (use of the streptomycin-resistant mutants). Results of screening of the diverse collection of 53 effective R. leguminosarum bv. viceae strains by the indirect method permits us to divide them into 3 groups (32 high-competitive, 10 medium-competitive and 11 low-competitive strains) but reveals no correlation between the competitiveness and symbiotic efficiency. N accumulation in the pea shoots is demonstrated to be a much more suitable criterion than the shoot mass for selection either of the highly-effective or of highly-competitive (by the indirect estimation) R. leguminosarum by. viceae strains in the pot experiments.

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Section: Increase In the Pea Yield And N Accumulation After Inoculatimentioning

confidence: 99%

Selection of Rhizobium leguminosarum bv. viceae strains for inoculation of Pisum sativum L. cultivars: Analysis of symbiotic efficiency and nodulation competitiveness

Фесенко¹,

Проворов

Orlova³

et al. 1995

Plant Soil

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“…On a 6tudid 12 souches de Rhl,zobium More recently, Munns (1965) and Barber (1980) found that some strains of R. meliLoti were more effective than others in nodulating alfalfa grown on moderately acid soils. The possibility of selecting lowpH tolerant strains of Rhizobium has often been discussed (Burton 1912Lte 1914, Munns et al .…”

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PERFORMANCE OF Rhizobium meliloti STRAINS SELECTED FOR LOW-pH TOLERANCE

Rice¹

1982

Can. J. Plant Sci.

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RtcE, W. A. 1982. Performance of Rhizobium meliloti strains selected for low-pH rolerance. Can. J. Plant Sci. 62:941_94g.Twelve strains of Rhizobitrm nteliloti were examined for their ability to nodulate alfalfa (Medicago sativa) and fix nitrogen under acid soil conditions. The strains were separated into a low-pH-sensitive group and a low-pH-tolerant group on the basis of plant dry matter yields in greenhouse experiments. The dry matter yields of alfalfa inoculated with the low-pH-tolerant strains were 5.9, 10.0 and 1.3 times greater than the yields with the low-pH-scnsitive strains at soil pH 5.0, 5.5 and 6.0, respectively. Yields of alfalfa at soil pH 6.7 werc the same for both groups.

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“…This behaviour might be explained by the findings of Barber (1980) who reported that the average number of R. meliloti decreased with both increasing acidity and the age of alfalfa stands.…”

Section: Resultsmentioning

confidence: 89%

Genetic diversity of Sinorhizobium meliloti associated with alfalfa in Chilean volcanic soils and their symbiotic effectiveness under acidic conditions

Langer

Nandasena

Howieson

et al. 2007

World J Microbiol Biotechnol

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A study was conducted with the aim of evaluating the genetic diversity of alfalfa rhizobia isolated from volcanic soils in southern Chile and their ability to establish an effective symbiosis with alfalfa. Rhizobial strains isolated from nodules were identified and selected based on PCR analyses and acid tolerance. Symbiotic effectiveness (nodulation and shoot dry weight) of acid-tolerant rhizobia was evaluated in glasshouse experiments under acidic conditions. The results revealed that Sinorhizobium meliloti is the dominant species in alfalfa nodules with a high genetic diversity at strain level grouped in three major clusters. There was a close relationship (r 2 = 0.895, P £ 0.001, n = 40) between soil pH and the size of rhizobial populations. Representative isolates from major cluster groups showed wide variation in acid tolerance expressed on buffered agar plates (pH 4.5-7.0) and symbiotic effectiveness with alfalfa. One isolate (NS11) appears to be suitable as an inoculant for alfalfa according to its acid tolerance and symbiotic effectiveness at low pH (5.5). The isolation and selection of naturalized S. meliloti strains with high symbiotic effectiveness under acidic conditions is an alternative approach to improving the productivity of alfalfa and for reducing the application of synthetic fertilizers in Chile.

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Enumeration, Effectiveness, and pH Resistance of Rhizobium meliloti Populations in Oregon Soils

Cited by 23 publications

References 0 publications

Selection of Rhizobium leguminosarum bv. viceae strains for inoculation of Pisum sativum L. cultivars: Analysis of symbiotic efficiency and nodulation competitiveness

Selection of Rhizobium leguminosarum bv. viceae strains for inoculation of Pisum sativum L. cultivars: Analysis of symbiotic efficiency and nodulation competitiveness

PERFORMANCE OF Rhizobium meliloti STRAINS SELECTED FOR LOW-pH TOLERANCE

Genetic diversity of Sinorhizobium meliloti associated with alfalfa in Chilean volcanic soils and their symbiotic effectiveness under acidic conditions

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