Enumeration and immunohistochemical characterisation of bone marrow basophils in myeloproliferative disorders using the basophil specific monoclonal antibody 2D7

Abstract

A novel immunohistochemical procedure has been established for basophil detection in normal bone marrow and MPD. This approach should help in the quantification of bone marrow basophils at diagnosis and during anti-leukaemic treatment.

The underestimated role of basophils in Ph+ chronic myeloid leukaemia

“…However, a number of useful immunohistochemical basophil stains that work in paraffin‐embedded BM section material, have been developed. These markers include the 2D7 antigen and the BB1 antigen, also known as basogranulin 48, 49. In addition, immature BM basophils express KIT and tryptase 50.…”

“…In addition, immature BM basophils express KIT and tryptase 50. It has also been described that basophils in CML can be detected and enumerated by 2D7 or BB1 staining and that the numbers of 2D7 +  and BB1 +  cells (basophils) correlate with the phase of CML 48, 49. However, both antibodies may also react with immature eosinophils in the leukaemic BM (P. Valent and H.‐P.…”

The CML-related oncoprotein BCR/ABL induces expression of histidine decarboxylase (HDC) and the synthesis of histamine in leukemic cells

“…Immunohistochemistry was performed using the indirect immunoperoxidase-staining technique, an anti-HDC antibody (work dilution, 1:500) and the basophil-specific mAb 2D7 (work dilution, 1:500) as described. [40][41][42][43] For ICC, cells (primary CML cells, cell lines) were spun on cytospin slides and stained with the anti-HDC antibody or 2D7 antibody as described. [40][41][42][43] Acquisition of figures was performed by an Olympus DP11 camera connected to an Olympus microscope equipped with 40ϫ/0.85 ( Figure 1A, B) and 100ϫ/1.35 ( Figure 1C, D) UPlan-Apo objective lenses (Olympus, Hamburg, Germany).…”

Eosinophil, basophil, and mast cell infiltrates in the bone marrow: crossing the boundaries of diagnosis

“…However, immunohistochemistry enables detection and quantification of basophils either using antibodies against basophil-specific antigens like 2D7 and BB1 or a combination of antibodies against tryptase and CD117 (KIT) since neoplastic basophils have been found to express the mast cell-related antigen tryptase but not CD117 which is otherwise constitutively expressed on mast cells [7][8][9]. Thus, small round tryptase-positive/CD117-negative cells can be regarded as basophils in histological sections.…”