ENU-induced late-onset night blindness associated with rod photoreceptor cell degeneration in zebrafish

Maaswinkel, Hans; Mason, B J; Li, Lei

doi:10.1016/j.mad.2003.08.004

Cited by 24 publications

(17 citation statements)

References 29 publications

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“…The escape response assay demonstrated that albino and ruby zebrafish, both pigmentation mutants, have reduced visual responses in bright light conditions 11,12 . This same escape response assay has also successfully identified night blindness d mutants, albeit at 2 years of age 13 . However, the escape response assay is not without flaws.…”

Section: Introductionmentioning

confidence: 87%

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Cameron

Rassamdana

Tam

et al. 2013

JoVE

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Zebrafish are a proven model for vision research, however many of the earlier methods generally focused on larval fish or demonstrated a simple response. More recently adult visual behavior in zebrafish has become of interest, but methods to measure specific responses are new coming. To address this gap, we set out to develop a methodology to repeatedly and accurately utilize the optokinetic response (OKR) to measure visual acuity in adult zebrafish. Here we show that the adult zebrafish's visual acuity can be measured, including both binocular and monocular acuities. Because the fish is not harmed during the procedure, the visual acuity can be measured and compared over short or long periods of time. The visual acuity measurements described here can also be done quickly allowing for high throughput and for additional visual procedures if desired. This type of analysis is conducive to drug intervention studies or investigations of disease progression.

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Section: Introductionmentioning

confidence: 87%

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Cameron

Rassamdana

Tam

et al. 2013

JoVE

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show abstract

“…Li and colleagues used the escape response, which is testable in zebrafish at 2 to 3 months of age, to measure the visual threshold of F1 fish derived from ENU-mutagenized males [8,[53][54][55]. Three mutants, night blindness a (nba), night blindness c (nbc), and night blindness d (nbd) displayed reduced escape responses in dim light as a result of photoreceptor dysfunction.…”

Section: Photoreceptor Mutants Isolated In Zebrafish Genetic Screensmentioning

confidence: 99%

Studying rod photoreceptor development in zebrafish

Morris

Fadool

2005

Physiology & Behavior

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The zebrafish has rapidly become a favored model vertebrate organism, well suited for studies of developmental processes using large-scale genetic screens. In particular, zebrafish morphological and behavioral genetic screens have led to the identification of genes important for development of the retinal photoreceptors. This may help clarify the genetic mechanisms underlying human photoreceptor development and dysfunction in retinal diseases. In this review, we present the advantages of zebrafish as a vertebrate model organism, summarize retinal and photoreceptor cell development in zebrafish, with emphasis on the rod photoreceptors, and describe zebrafish visual behaviors that can be used for genetic screens. We then describe some of the photoreceptor cell mutants that have been isolated in morphological and behavioral screens and discuss the limitations of current screening methods for uncovering mutations that specifically affect rod function. Finally, we present some alternative strategies to target the rod developmental pathway in zebrafish.

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“…Zebrafish also do not possess iris muscles, instead, the zebrafish iris contains several types of pigment cells that may function in preventing light from entering the eye outside of the pupil (Soules and Link, 2005). Nonetheless, there is Karlstrom et al (1996) brudas (bru) Unknown -Photoreceptor defects Malicki et al (1996) cloche (clo) Cataracts Goishi et al (2006) elipsa (eli) Unknown -Photoreceptor defects Malicki et al (1996) fade out (fad) Unknown -RPE/retinal degeneration Bahadori et al (2006) fading vision (fdv) silva 193500 RPE pigmentation, visual function (embryonic) Schonthaler et al (2005) fleer (flr) Unknown -Photoreceptor defects Malicki et al (1996) gantenbein ( mikre oko (mok) Unknown -Photoreceptor defects Malicki et al (1996) n-cadherin (ncad) ncad -Coloboma, retinal disorganization Masai et al (2003); Erdmann et al (2003); Malicki et al (2003) niezerka (nie) Unknown -Photoreceptor defects Malicki et al (1996) Night blindness (nba, nbb, nbc, nbd, nbe, nbf, nbg) Unknown -Night blindness (heterozygotes) Li andDowling (1997, 2000); Maaswinkel et al (Maaswinkel et al, 2003a(Maaswinkel et al, ,b, 2005 no optokinetic response a (noa) dlat 245348 Visual function Taylor et al (2004) no optokinetic response f (nof) gnat2 139340 Visual function …”

Section: Development and Anatomy Of The Zebrafish Eyementioning

confidence: 99%

“…Night blindness can result from nondegenerative diseases (i.e., congenital stationary night blindness) or degenerative diseases such as retinitis pigmentosa. Screens utilizing the escape response assay have identified seven night blindness loci (nba, nbb, nbc, nbd, nbe, nbf, nbg) (Li andDowling, 1997, 2000;Maaswinkel et al, 2003aMaaswinkel et al, ,b, 2005. Six of the seven heterozygous mutants (all but nbb) exhibit some degree of photoreceptor degeneration; however, the degree of degeneration varies dramatically between mutants.…”

Section: Anterior Segment Disorders-cataractsmentioning

confidence: 99%